Molecular evaluation of endothelial progenitor cells in patients with ischemic limbs: therapeutic effect by stem cell transplantation.

Koji Yamamoto, Takahisa Kondo, Satoshi Suzuki, Hideo Izawa, Masayoshi Kobayashi, Nobuhiko Emi, Kimihiro Komori, Tomoki Naoe, Junki Takamatsu, Toyoaki Murohara

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

OBJECTIVE: Although some patients with limb ischemia have recently undergone therapeutic angiogenesis by cell transplantation, their angiogenic potential has not been well characterized. It is also important to evaluate endothelial progenitor cell (EPC) contents in different stem cell sources to choose the best material for therapeutic angiogenesis. METHODS AND RESULTS: We quantitated the mRNA expression of EPC-specific molecules (eg, Flk-1, Flt-1, CD133, VE-cadherin, etc) in bone marrow-derived or peripheral blood-derived mononuclear cells obtained from patients with ischemic limbs, using real-time reverse-transcription polymerase chain reaction technique. The mRNA expression level of EPC markers was significantly lower in the patients than in healthy controls, which was consistent with results of flow cytometric analysis. However, the implantation of autologous bone marrow mononuclear cells increased the circulating EPCs in the peripheral blood of patients. We furthermore revealed the different expression pattern of EPC markers in possible sources for stem cell transplantation, including normal bone marrow, peripheral blood obtained from recombinant granulocyte colony-stimulating factor-treated donor, and umbilical cord blood. CONCLUSIONS: Patients with peripheral obstructive arterial diseases may have lower angiogenic potential because of decreased expression of EPC specific molecules in their marrow and blood. Therapeutic angiogenesis by transplantation of autologous marrow mononuclear cells increased circulating EPCs in the patients and improved ischemic symptoms.

Original languageEnglish
Pages (from-to)e192-196
JournalArteriosclerosis, thrombosis, and vascular biology
Volume24
Issue number12
Publication statusPublished - 12-2004

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Stem Cell Transplantation
Therapeutic Uses
Extremities
Bone Marrow
Messenger RNA
Autologous Transplantation
Peripheral Arterial Disease
Cell Transplantation
Granulocyte Colony-Stimulating Factor
Fetal Blood
Bone Marrow Cells
Reverse Transcription
Endothelial Progenitor Cells
Stem Cells
Therapeutics
Ischemia
Tissue Donors
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Yamamoto, Koji ; Kondo, Takahisa ; Suzuki, Satoshi ; Izawa, Hideo ; Kobayashi, Masayoshi ; Emi, Nobuhiko ; Komori, Kimihiro ; Naoe, Tomoki ; Takamatsu, Junki ; Murohara, Toyoaki. / Molecular evaluation of endothelial progenitor cells in patients with ischemic limbs : therapeutic effect by stem cell transplantation. In: Arteriosclerosis, thrombosis, and vascular biology. 2004 ; Vol. 24, No. 12. pp. e192-196.
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abstract = "OBJECTIVE: Although some patients with limb ischemia have recently undergone therapeutic angiogenesis by cell transplantation, their angiogenic potential has not been well characterized. It is also important to evaluate endothelial progenitor cell (EPC) contents in different stem cell sources to choose the best material for therapeutic angiogenesis. METHODS AND RESULTS: We quantitated the mRNA expression of EPC-specific molecules (eg, Flk-1, Flt-1, CD133, VE-cadherin, etc) in bone marrow-derived or peripheral blood-derived mononuclear cells obtained from patients with ischemic limbs, using real-time reverse-transcription polymerase chain reaction technique. The mRNA expression level of EPC markers was significantly lower in the patients than in healthy controls, which was consistent with results of flow cytometric analysis. However, the implantation of autologous bone marrow mononuclear cells increased the circulating EPCs in the peripheral blood of patients. We furthermore revealed the different expression pattern of EPC markers in possible sources for stem cell transplantation, including normal bone marrow, peripheral blood obtained from recombinant granulocyte colony-stimulating factor-treated donor, and umbilical cord blood. CONCLUSIONS: Patients with peripheral obstructive arterial diseases may have lower angiogenic potential because of decreased expression of EPC specific molecules in their marrow and blood. Therapeutic angiogenesis by transplantation of autologous marrow mononuclear cells increased circulating EPCs in the patients and improved ischemic symptoms.",
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Yamamoto, K, Kondo, T, Suzuki, S, Izawa, H, Kobayashi, M, Emi, N, Komori, K, Naoe, T, Takamatsu, J & Murohara, T 2004, 'Molecular evaluation of endothelial progenitor cells in patients with ischemic limbs: therapeutic effect by stem cell transplantation.', Arteriosclerosis, thrombosis, and vascular biology, vol. 24, no. 12, pp. e192-196.

Molecular evaluation of endothelial progenitor cells in patients with ischemic limbs : therapeutic effect by stem cell transplantation. / Yamamoto, Koji; Kondo, Takahisa; Suzuki, Satoshi; Izawa, Hideo; Kobayashi, Masayoshi; Emi, Nobuhiko; Komori, Kimihiro; Naoe, Tomoki; Takamatsu, Junki; Murohara, Toyoaki.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 24, No. 12, 12.2004, p. e192-196.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecular evaluation of endothelial progenitor cells in patients with ischemic limbs

T2 - therapeutic effect by stem cell transplantation.

AU - Yamamoto, Koji

AU - Kondo, Takahisa

AU - Suzuki, Satoshi

AU - Izawa, Hideo

AU - Kobayashi, Masayoshi

AU - Emi, Nobuhiko

AU - Komori, Kimihiro

AU - Naoe, Tomoki

AU - Takamatsu, Junki

AU - Murohara, Toyoaki

PY - 2004/12

Y1 - 2004/12

N2 - OBJECTIVE: Although some patients with limb ischemia have recently undergone therapeutic angiogenesis by cell transplantation, their angiogenic potential has not been well characterized. It is also important to evaluate endothelial progenitor cell (EPC) contents in different stem cell sources to choose the best material for therapeutic angiogenesis. METHODS AND RESULTS: We quantitated the mRNA expression of EPC-specific molecules (eg, Flk-1, Flt-1, CD133, VE-cadherin, etc) in bone marrow-derived or peripheral blood-derived mononuclear cells obtained from patients with ischemic limbs, using real-time reverse-transcription polymerase chain reaction technique. The mRNA expression level of EPC markers was significantly lower in the patients than in healthy controls, which was consistent with results of flow cytometric analysis. However, the implantation of autologous bone marrow mononuclear cells increased the circulating EPCs in the peripheral blood of patients. We furthermore revealed the different expression pattern of EPC markers in possible sources for stem cell transplantation, including normal bone marrow, peripheral blood obtained from recombinant granulocyte colony-stimulating factor-treated donor, and umbilical cord blood. CONCLUSIONS: Patients with peripheral obstructive arterial diseases may have lower angiogenic potential because of decreased expression of EPC specific molecules in their marrow and blood. Therapeutic angiogenesis by transplantation of autologous marrow mononuclear cells increased circulating EPCs in the patients and improved ischemic symptoms.

AB - OBJECTIVE: Although some patients with limb ischemia have recently undergone therapeutic angiogenesis by cell transplantation, their angiogenic potential has not been well characterized. It is also important to evaluate endothelial progenitor cell (EPC) contents in different stem cell sources to choose the best material for therapeutic angiogenesis. METHODS AND RESULTS: We quantitated the mRNA expression of EPC-specific molecules (eg, Flk-1, Flt-1, CD133, VE-cadherin, etc) in bone marrow-derived or peripheral blood-derived mononuclear cells obtained from patients with ischemic limbs, using real-time reverse-transcription polymerase chain reaction technique. The mRNA expression level of EPC markers was significantly lower in the patients than in healthy controls, which was consistent with results of flow cytometric analysis. However, the implantation of autologous bone marrow mononuclear cells increased the circulating EPCs in the peripheral blood of patients. We furthermore revealed the different expression pattern of EPC markers in possible sources for stem cell transplantation, including normal bone marrow, peripheral blood obtained from recombinant granulocyte colony-stimulating factor-treated donor, and umbilical cord blood. CONCLUSIONS: Patients with peripheral obstructive arterial diseases may have lower angiogenic potential because of decreased expression of EPC specific molecules in their marrow and blood. Therapeutic angiogenesis by transplantation of autologous marrow mononuclear cells increased circulating EPCs in the patients and improved ischemic symptoms.

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