Molecular genetic analysis of rat TCR chain - Identification of an invariant T cell receptor by 5′RACE

Hong Zhi Chen, Akihiro Matsuura, Noriyuki Sato

Research output: Contribution to journalArticle

Abstract

To investigate rat T cell receptor, we adapted the 5′RACE (rapid amplificaton of cDNA ends) method, which has been proven as effective in analysis of TCRα chain. Here, a new TCR α chain gene was first identified in rat, and designated as rat Vα14Jα281 (rV α14Jα281) gene because of its high sequence similarity to mouse V α14Jα281 and human V α24JαQ T cell receptor α chain gene. The similarity of rV α14Jα281 with mV α14Jα281 and with V α24JαQ is 88.3%, and 76.0%, respectively. The rV α14 gene rearrangement with rJ α281 has a non-diversity N-region at the VJ junction, named as rat invariant V α14Jα281 (invariant rV α14Jα281). Our investigation also shown that invariant rV α14Jα281 was frequently expressed in total V α14 + TCR at extrathymic sites in 36.1% and 94.6% of splenocytes and hepatic lymphocytes, respectively, although the frequency of invariant rV α14Jα281 is 5.4% in the thymus. Subsequently we investigated the expression of invariant rV α14Jα281 TCR in nude rat which lacked thymus. The data suggest that a unique lineage of invariant rV α14Jα281 TCR + T cells matured through a selection process different from conventional αβ T cells and have an essential regulatory function for immunological responses.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalSapporo Medical Journal
Volume68
Issue number1-3
Publication statusPublished - 01-06-1999

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T-Cell Antigen Receptor
Molecular Biology
Thymus Gland
invariant chain
Nude Rats
T-Lymphocytes
T-Cell Receptor Genes
Gene Rearrangement
Genes
Complementary DNA
Lymphocytes
Liver

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Molecular genetic analysis of rat TCR chain - Identification of an invariant T cell receptor by 5′RACE",
abstract = "To investigate rat T cell receptor, we adapted the 5′RACE (rapid amplificaton of cDNA ends) method, which has been proven as effective in analysis of TCRα chain. Here, a new TCR α chain gene was first identified in rat, and designated as rat Vα14Jα281 (rV α14Jα281) gene because of its high sequence similarity to mouse V α14Jα281 and human V α24JαQ T cell receptor α chain gene. The similarity of rV α14Jα281 with mV α14Jα281 and with V α24JαQ is 88.3{\%}, and 76.0{\%}, respectively. The rV α14 gene rearrangement with rJ α281 has a non-diversity N-region at the VJ junction, named as rat invariant V α14Jα281 (invariant rV α14Jα281). Our investigation also shown that invariant rV α14Jα281 was frequently expressed in total V α14 + TCR at extrathymic sites in 36.1{\%} and 94.6{\%} of splenocytes and hepatic lymphocytes, respectively, although the frequency of invariant rV α14Jα281 is 5.4{\%} in the thymus. Subsequently we investigated the expression of invariant rV α14Jα281 TCR in nude rat which lacked thymus. The data suggest that a unique lineage of invariant rV α14Jα281 TCR + T cells matured through a selection process different from conventional αβ T cells and have an essential regulatory function for immunological responses.",
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Molecular genetic analysis of rat TCR chain - Identification of an invariant T cell receptor by 5′RACE. / Chen, Hong Zhi; Matsuura, Akihiro; Sato, Noriyuki.

In: Sapporo Medical Journal, Vol. 68, No. 1-3, 01.06.1999, p. 1-13.

Research output: Contribution to journalArticle

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