Molecular responses of maize shoot to a plant derived smoke solution

Muhammad Mudasar Aslam, Shafiq Rehman, Amana Khatoon, Muhammad Jamil, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida, Xinyue Li, Yukari Sunohara, Hiroshi Matsumoto, Setsuko Komatsu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Plant-derived smoke has effects on plant growth. To find the molecular mechanism of plant-derived smoke on maize, a gel-free/label-free proteomic technique was used. The length of root and shoot were increased in maize by plant-derived smoke. Proteomic analysis revealed that 2000 ppm plant-derived smoke changed the abundance of 69 proteins in 4-days old maize shoot. Proteins in cytoplasm, chloroplast, and cell membrane were altered by plant-derived smoke. Catalytic, signaling, and nucleotide binding proteins were changed. Proteins related to sucrose synthase, nucleotides, signaling, and glutathione were significantly increased; however, cell wall, lipids, photosynthetic, and amino acid degradations related proteins were decreased. Based on proteomic and immunoblot analyses, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) was decreased; however, RuBisCO activase was not changed by plant-derived smoke in maize shoot. Ascorbate peroxidase was not affected; however, peroxiredoxin was decreased by plant-derived smoke. Furthermore, the results from enzyme-activity and mRNA-expression analyses confirmed regulation of ascorbate peroxidase and the peroxiredoxinin reactive oxygen scavenging system. These results suggest that increases in sucrose synthase, nucleotides, signaling, and glutathione related proteins combined with regulation of reactive oxygen species and their scavenging system in response to plant-derived smoke may improve maize growth.

Original languageEnglish
Article number1319
JournalInternational journal of molecular sciences
Volume20
Issue number6
DOIs
Publication statusPublished - 02-03-2019

Fingerprint

smoke
Smoke
Zea mays
proteins
Proteins
nucleotides
Nucleotides
Ascorbate Peroxidases
Proteomics
Oxygenases
glutathione
sucrose
Scavenging
scavenging
Sugar (sucrose)
acids
Glutathione
chloroplasts
membranes
Peroxiredoxins

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Aslam, Muhammad Mudasar ; Rehman, Shafiq ; Khatoon, Amana ; Jamil, Muhammad ; Yamaguchi, Hisateru ; Hitachi, Keisuke ; Tsuchida, Kunihiro ; Li, Xinyue ; Sunohara, Yukari ; Matsumoto, Hiroshi ; Komatsu, Setsuko. / Molecular responses of maize shoot to a plant derived smoke solution. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 6.
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Aslam, MM, Rehman, S, Khatoon, A, Jamil, M, Yamaguchi, H, Hitachi, K, Tsuchida, K, Li, X, Sunohara, Y, Matsumoto, H & Komatsu, S 2019, 'Molecular responses of maize shoot to a plant derived smoke solution', International journal of molecular sciences, vol. 20, no. 6, 1319. https://doi.org/10.3390/ijms20061319

Molecular responses of maize shoot to a plant derived smoke solution. / Aslam, Muhammad Mudasar; Rehman, Shafiq; Khatoon, Amana; Jamil, Muhammad; Yamaguchi, Hisateru; Hitachi, Keisuke; Tsuchida, Kunihiro; Li, Xinyue; Sunohara, Yukari; Matsumoto, Hiroshi; Komatsu, Setsuko.

In: International journal of molecular sciences, Vol. 20, No. 6, 1319, 02.03.2019.

Research output: Contribution to journalArticle

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AU - Aslam, Muhammad Mudasar

AU - Rehman, Shafiq

AU - Khatoon, Amana

AU - Jamil, Muhammad

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AU - Hitachi, Keisuke

AU - Tsuchida, Kunihiro

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AU - Komatsu, Setsuko

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