TY - JOUR
T1 - Molecular structure of bacterial endotoxin (Escherichia coli Re lipopolysaccharide)
T2 - Implications for formation of a novel heterogenous lattice structure
AU - Kato, Nobuo
AU - Sugiyama, Tsuyoshi
AU - Naito, Setsuko
AU - Arakawa, Yoshichika
AU - Ito, Hideo
AU - Kido, Nobuo
AU - Ohta, Michio
AU - Sasaki, Kyoyu
PY - 2000
Y1 - 2000
N2 - Analyses of crystals of Escherichia coli Re lipopolysaccharide (LPS) formed after storage in 1% triethylamine indicate that the LPS molecules are assembled to form a monolayered structure consisting of a novel heterogeneous lattice structure, the greater part of which is occupied by one kind of lattice (lattice I), corresponding to the acyl chain portion of lipid A, and the remainder is occupied by the other kind of lattice (lattice II), corresponding to the 3-deoxy-D-manno-octulosonic acid (dOclA) dimer and the M-acetylglucosamine disaccharide of lipid A. X-ray diffraction reveals that the type of cell is monoclinic (a = 5.53 Å, b = 27.2 Å, c = 6.47 Å, α = 90°, β = 125.8°, γ = 90°). Atomic force microscopy shows that crystals consist of multiple layers; the thickness of a layer corresponds to the b-axis value, and two types of surface topographies are visualized one, regarded as the view onto the acyl chain ends, is two-dimensional arrays of oval bodies that constitute the lattice, with the lattice constants corresponding to the a- and c-axes and the angle of β (lattice I). The other, regarded as the view onto the dOclA dimers, is two-dimensional arrays of dromedary-back-like bodies that constitute the lattice with axes of 9.0 and 10.7 Å and the angle of 65°formed by both axes (lattice II). Based on these results, we present the molecular model of E. coli Re LPS.
AB - Analyses of crystals of Escherichia coli Re lipopolysaccharide (LPS) formed after storage in 1% triethylamine indicate that the LPS molecules are assembled to form a monolayered structure consisting of a novel heterogeneous lattice structure, the greater part of which is occupied by one kind of lattice (lattice I), corresponding to the acyl chain portion of lipid A, and the remainder is occupied by the other kind of lattice (lattice II), corresponding to the 3-deoxy-D-manno-octulosonic acid (dOclA) dimer and the M-acetylglucosamine disaccharide of lipid A. X-ray diffraction reveals that the type of cell is monoclinic (a = 5.53 Å, b = 27.2 Å, c = 6.47 Å, α = 90°, β = 125.8°, γ = 90°). Atomic force microscopy shows that crystals consist of multiple layers; the thickness of a layer corresponds to the b-axis value, and two types of surface topographies are visualized one, regarded as the view onto the acyl chain ends, is two-dimensional arrays of oval bodies that constitute the lattice, with the lattice constants corresponding to the a- and c-axes and the angle of β (lattice I). The other, regarded as the view onto the dOclA dimers, is two-dimensional arrays of dromedary-back-like bodies that constitute the lattice with axes of 9.0 and 10.7 Å and the angle of 65°formed by both axes (lattice II). Based on these results, we present the molecular model of E. coli Re LPS.
UR - http://www.scopus.com/inward/record.url?scp=0034034856&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034034856&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2958.2000.01893.x
DO - 10.1046/j.1365-2958.2000.01893.x
M3 - Article
C2 - 10844668
AN - SCOPUS:0034034856
SN - 0950-382X
VL - 36
SP - 796
EP - 805
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 4
ER -