Monitoring of active HHV-6 infection in bone marrow transplant recipients by real time PCR; comparison to detection of viral DNA in plasma by qualitative PCR

Masaru Ihira, Tetsushi Yoshikawa, Kyoko Suzuki, Masahiro Ohashi, Sadao Suga, Keizo Horibe, Naoko Tanaka, Hiroshi Kimura, Seiji Kojima, Koji Kato, Takaharu Matsuyama, Yukihiro Nishiyama, Yoshizo Asano

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Twelve (46%) of the 26 patients had human herpesvirus 6 (HHV-6) viremia after bone marrow transplant (BMT). All isolates were recovered from the samples obtained at 2 weeks after BMT. The sensitivity and the specificity of detection of viral DNA in plasma by qualitative polymerase chain reaction (PCR) for monitoring active virus replication were 92% and 97% respectively. Moreover, the positive (85%) and negative (99%) predictive values were also high. The patients with HHV-6 viremia showed a clear peak in HHV-6 DNA in peripheral blood mononuclear cells (PBMCs) at 2 weeks after BMT, which was measured by real time PCR. The virus DNA level in PBMCs between the two groups (patients with viremia and patients without viremia) was statistically different at 2 weeks after BMT (P = 0.033). In patients with HHV-6 viremia, mean HHV-6 DNA copy number was higher in the samples collected at 2 weeks after BMT than the samples collected at any other time period.

Original languageEnglish
Pages (from-to)701-705
Number of pages5
Issue number10
Publication statusPublished - 01-01-2002
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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