Monoclonal antibodies in man that neutralized H3N2 influenza viruses were classified into three groups with distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003

Jun Okada; Nobuko Ohshima; Ritsuko Kubota-Koketsu; Sayuri Ota; Wakana Takase; Masachika Azuma; Yoshitaka Iba; Naoko Nakagawa; Tetsushi Yoshikawa; Youichi Nakajima; Toyokazu Ishikawa; Yoshizo Asano; Yoshinobu Okuno; Yoshikazu Kurosawa

doi:10.1016/j.virol.2009.11.025

Monoclonal antibodies in man that neutralized H3N2 influenza viruses were classified into three groups with distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003

Jun Okada, Nobuko Ohshima, Ritsuko Kubota-Koketsu, Sayuri Ota, Wakana Takase, Masachika Azuma, Yoshitaka Iba, Naoko Nakagawa, Tetsushi Yoshikawa, Youichi Nakajima, Toyokazu Ishikawa, Yoshizo Asano, Yoshinobu Okuno, Yoshikazu Kurosawa

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

We tried to reveal the strain specificity of neutralizing mAbs against H3N2 influenza viruses in individuals. A large number of B lymphocytes of a pediatrician were collected by apheresis and two Ab libraries were constructed at 2004 and 2007 by using the phage-display technology. The libraries were screened against 12 different H3 strains of flu isolated between 1968 and 2004. Large numbers of clones that bound to the Ags were isolated and mAbs that specifically bound to H3 strain viruses were selected. Their binding activity to the 12 strains and neutralizing activity were studied by ELISA and focus reduction test, respectively. Furthermore, the binding activity to hemagglutinin (HA) was examined by Western blot. The majority of clones showing the neutralizing activity turned out to be anti-HA mAbs and could be divided into three major groups showing distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003.

Original language	English
Pages (from-to)	322-330
Number of pages	9
Journal	Virology
Volume	397
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2009.11.025
Publication status	Published - 20-02-2010

All Science Journal Classification (ASJC) codes

Virology

Access to Document

10.1016/j.virol.2009.11.025

Cite this

Okada, J., Ohshima, N., Kubota-Koketsu, R., Ota, S., Takase, W., Azuma, M., Iba, Y., Nakagawa, N., Yoshikawa, T., Nakajima, Y., Ishikawa, T., Asano, Y., Okuno, Y., & Kurosawa, Y. (2010). Monoclonal antibodies in man that neutralized H3N2 influenza viruses were classified into three groups with distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003. Virology, 397(2), 322-330. https://doi.org/10.1016/j.virol.2009.11.025

@article{1d21e1a0a5e34f589da5a55d0e41be7a,

title = "Monoclonal antibodies in man that neutralized H3N2 influenza viruses were classified into three groups with distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003",

abstract = "We tried to reveal the strain specificity of neutralizing mAbs against H3N2 influenza viruses in individuals. A large number of B lymphocytes of a pediatrician were collected by apheresis and two Ab libraries were constructed at 2004 and 2007 by using the phage-display technology. The libraries were screened against 12 different H3 strains of flu isolated between 1968 and 2004. Large numbers of clones that bound to the Ags were isolated and mAbs that specifically bound to H3 strain viruses were selected. Their binding activity to the 12 strains and neutralizing activity were studied by ELISA and focus reduction test, respectively. Furthermore, the binding activity to hemagglutinin (HA) was examined by Western blot. The majority of clones showing the neutralizing activity turned out to be anti-HA mAbs and could be divided into three major groups showing distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003.",

author = "Jun Okada and Nobuko Ohshima and Ritsuko Kubota-Koketsu and Sayuri Ota and Wakana Takase and Masachika Azuma and Yoshitaka Iba and Naoko Nakagawa and Tetsushi Yoshikawa and Youichi Nakajima and Toyokazu Ishikawa and Yoshizo Asano and Yoshinobu Okuno and Yoshikazu Kurosawa",

note = "Funding Information: We are grateful to Dr. Abraham Karpas for his precious support. This work was supported in part by a grant-in-aid for the 21st Century Center of Excellence (COE) program of Fujita Health University from the Ministry of Education, Culture, Sports, Science and Technology and a grant for Research on Pharmaceutical and Medical Safety from the Ministry of Health, Labor, and Welfare of Japan. ",

year = "2010",

month = feb,

day = "20",

doi = "10.1016/j.virol.2009.11.025",

language = "English",

volume = "397",

pages = "322--330",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

number = "2",

}

Okada, J, Ohshima, N, Kubota-Koketsu, R, Ota, S, Takase, W, Azuma, M, Iba, Y, Nakagawa, N, Yoshikawa, T, Nakajima, Y, Ishikawa, T, Asano, Y, Okuno, Y & Kurosawa, Y 2010, 'Monoclonal antibodies in man that neutralized H3N2 influenza viruses were classified into three groups with distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003', Virology, vol. 397, no. 2, pp. 322-330. https://doi.org/10.1016/j.virol.2009.11.025

TY - JOUR

T1 - Monoclonal antibodies in man that neutralized H3N2 influenza viruses were classified into three groups with distinct strain specificity

T2 - 1968-1973, 1977-1993 and 1997-2003

AU - Okada, Jun

AU - Ohshima, Nobuko

AU - Kubota-Koketsu, Ritsuko

AU - Ota, Sayuri

AU - Takase, Wakana

AU - Azuma, Masachika

AU - Iba, Yoshitaka

AU - Nakagawa, Naoko

AU - Yoshikawa, Tetsushi

AU - Nakajima, Youichi

AU - Ishikawa, Toyokazu

AU - Asano, Yoshizo

AU - Okuno, Yoshinobu

AU - Kurosawa, Yoshikazu

N1 - Funding Information: We are grateful to Dr. Abraham Karpas for his precious support. This work was supported in part by a grant-in-aid for the 21st Century Center of Excellence (COE) program of Fujita Health University from the Ministry of Education, Culture, Sports, Science and Technology and a grant for Research on Pharmaceutical and Medical Safety from the Ministry of Health, Labor, and Welfare of Japan.

PY - 2010/2/20

Y1 - 2010/2/20

N2 - We tried to reveal the strain specificity of neutralizing mAbs against H3N2 influenza viruses in individuals. A large number of B lymphocytes of a pediatrician were collected by apheresis and two Ab libraries were constructed at 2004 and 2007 by using the phage-display technology. The libraries were screened against 12 different H3 strains of flu isolated between 1968 and 2004. Large numbers of clones that bound to the Ags were isolated and mAbs that specifically bound to H3 strain viruses were selected. Their binding activity to the 12 strains and neutralizing activity were studied by ELISA and focus reduction test, respectively. Furthermore, the binding activity to hemagglutinin (HA) was examined by Western blot. The majority of clones showing the neutralizing activity turned out to be anti-HA mAbs and could be divided into three major groups showing distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003.

AB - We tried to reveal the strain specificity of neutralizing mAbs against H3N2 influenza viruses in individuals. A large number of B lymphocytes of a pediatrician were collected by apheresis and two Ab libraries were constructed at 2004 and 2007 by using the phage-display technology. The libraries were screened against 12 different H3 strains of flu isolated between 1968 and 2004. Large numbers of clones that bound to the Ags were isolated and mAbs that specifically bound to H3 strain viruses were selected. Their binding activity to the 12 strains and neutralizing activity were studied by ELISA and focus reduction test, respectively. Furthermore, the binding activity to hemagglutinin (HA) was examined by Western blot. The majority of clones showing the neutralizing activity turned out to be anti-HA mAbs and could be divided into three major groups showing distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003.

UR - http://www.scopus.com/inward/record.url?scp=75749120153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75749120153&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2009.11.025

DO - 10.1016/j.virol.2009.11.025

M3 - Article

C2 - 19969317

AN - SCOPUS:75749120153

SN - 0042-6822

VL - 397

SP - 322

EP - 330

JO - Virology

JF - Virology

IS - 2

ER -

Monoclonal antibodies in man that neutralized H3N2 influenza viruses were classified into three groups with distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this