Morphologic Analysis of M2 Macrophage in Glioblastoma: Involvement of Macrophage Extracellular Traps (METs)

Ayano Michiba, Kazuya Shiogama, Tetsuya Tsukamoto, Masaya Hirayama, Seiji Yamada, Masato Abe

Research output: Contribution to journalArticlepeer-review

Abstract

Macrophages are classified into two phenotypes, M1 and M2, based on their roles. M2 macrophages suppress inflammation and increase in proportion to the malignancy of brain tumors. Recently, macrophage extracellular traps (METs), which change into a network, have been reported as a unique form of macrophage cell death. In this study, immunohisto-chemical analysis of macrophages in METs in human glioblastoma was performed. To distin-guish between M1 and M2 macrophages, multiple immunostainings with Iba1 combined with CD163 or CD204 were performed. M2 macrophages were present in small amounts in normal and borderline areas but showed an increasing trend as they shifted to tumor areas, and most of them were the activated-or phagocytic-type. We also successfully detected METs coexisting with fibrin and lactoferrin near the border between the tumor and necrotic area. M2 macrophages not only suppressed inflammation but also were involved in the formation of METs. This study found that M2 macrophages play various roles in unstable situations.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalActa Histochemica et Cytochemica
Volume55
Issue number4
DOIs
Publication statusPublished - 2022

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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