Morphological asymmetry in dividing retinal progenitor cells

Kanako Saito, Ayano Kawaguchi, Saori Kashiwagi, Sadao Yasugi, Masaharu Ogawa, Takaki Miyata

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

For the understanding of histogenetic events in the 3-D retinal neuroepithelium, direct observation of the progenitor cells and their morphological changes is required. A slice culture method has been developed by which the behavior of single progenitor cells can be monitored. Although it has been believed that each retinal progenitor cell loses its basal process while it is in M phase, it is reported here that the process is retained throughout M phase and is inherited by one daughter cell, which can be a neuron or a progenitor cell. Daughter neurons used an inherited process for neuronal translocation and positioning. In divisions that produced two mitotic daughters, both of which subsequently divided to form four granddaughter cells, only one daughter cell inherited the original basal process while the other extended a new process. Interestingly, behavioral differences were often noted between such mitotic sisters in the trajectory of interkinetic nuclear movement, cell cycle length, and the composition of the granddaughter pair. Therefore, 'symmetric' (progenitor → progenitor + progenitor) divisions are in fact morphologically asymmetric, and the behavior of the mitotic daughters can often be asymmetric, indicating the necessity for studying possible associations between the process inheritance and the cell fate choice.

Original languageEnglish
Pages (from-to)219-229
Number of pages11
JournalDevelopment Growth and Differentiation
Volume45
Issue number3
DOIs
Publication statusPublished - 06-2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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