Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14

Naoki Koide; Tsuyoshi Sugiyama; Yutaka Kato; Dipshika Chakravortty; Mya Mya Mu; Tomoaki Yoshida; Teruaki Hamano; Takashi Yokochi

doi:10.1177/09680519010070010601

Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14

Naoki Koide, Tsuyoshi Sugiyama, Yutaka Kato, Dipshika Chakravortty, Mya Mya Mu, Tomoaki Yoshida, Teruaki Hamano, Takashi Yokochi

Medical Biology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The role of membrane-bound CD14 in the response of mouse B1 cell lines to lipopolysaccharide (LPS) was studied. The surface profile of mouse TH2.52 B cells was positive for CD5, IgM, B220, CD11b and F4/80, suggesting that TH2.52 cells carried the typical phenotype of B1 cells. Furthermore, TH2.52 B1 cells were found to express membrane-bound CD14, which plays a critical role in LPS recognition. TH2.52 B1 cells responded to a very low concentration of LPS and exhibited: (i) augmentation of DNA synthesis; (ii) activation of nuclear factor (NF)-κB; and (iii) phosphorylation of extracellular signal regulated kinase 1/2 (Erk1/2). They were markedly inhibited by anti-CD14 antibody. Therefore, the expression of membrane-bound CD14 was suggested to provide high sensitivity to LPS for TH2.52 B1 cells.

Original language	English
Pages (from-to)	39-43
Number of pages	5
Journal	Journal of Endotoxin Research
Volume	7
Issue number	1
DOIs	https://doi.org/10.1177/09680519010070010601
Publication status	Published - 02-2001

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Molecular Biology
Cell Biology
Infectious Diseases

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1177/09680519010070010601

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title = "Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14",

abstract = "The role of membrane-bound CD14 in the response of mouse B1 cell lines to lipopolysaccharide (LPS) was studied. The surface profile of mouse TH2.52 B cells was positive for CD5, IgM, B220, CD11b and F4/80, suggesting that TH2.52 cells carried the typical phenotype of B1 cells. Furthermore, TH2.52 B1 cells were found to express membrane-bound CD14, which plays a critical role in LPS recognition. TH2.52 B1 cells responded to a very low concentration of LPS and exhibited: (i) augmentation of DNA synthesis; (ii) activation of nuclear factor (NF)-κB; and (iii) phosphorylation of extracellular signal regulated kinase 1/2 (Erk1/2). They were markedly inhibited by anti-CD14 antibody. Therefore, the expression of membrane-bound CD14 was suggested to provide high sensitivity to LPS for TH2.52 B1 cells.",

author = "Naoki Koide and Tsuyoshi Sugiyama and Yutaka Kato and Dipshika Chakravortty and Mu, {Mya Mya} and Tomoaki Yoshida and Teruaki Hamano and Takashi Yokochi",

year = "2001",

month = feb,

doi = "10.1177/09680519010070010601",

language = "English",

volume = "7",

pages = "39--43",

journal = "Journal of Endotoxin Research",

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T1 - Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14

AU - Koide, Naoki

AU - Sugiyama, Tsuyoshi

AU - Kato, Yutaka

AU - Chakravortty, Dipshika

AU - Mu, Mya Mya

AU - Yoshida, Tomoaki

AU - Hamano, Teruaki

AU - Yokochi, Takashi

PY - 2001/2

Y1 - 2001/2

N2 - The role of membrane-bound CD14 in the response of mouse B1 cell lines to lipopolysaccharide (LPS) was studied. The surface profile of mouse TH2.52 B cells was positive for CD5, IgM, B220, CD11b and F4/80, suggesting that TH2.52 cells carried the typical phenotype of B1 cells. Furthermore, TH2.52 B1 cells were found to express membrane-bound CD14, which plays a critical role in LPS recognition. TH2.52 B1 cells responded to a very low concentration of LPS and exhibited: (i) augmentation of DNA synthesis; (ii) activation of nuclear factor (NF)-κB; and (iii) phosphorylation of extracellular signal regulated kinase 1/2 (Erk1/2). They were markedly inhibited by anti-CD14 antibody. Therefore, the expression of membrane-bound CD14 was suggested to provide high sensitivity to LPS for TH2.52 B1 cells.

AB - The role of membrane-bound CD14 in the response of mouse B1 cell lines to lipopolysaccharide (LPS) was studied. The surface profile of mouse TH2.52 B cells was positive for CD5, IgM, B220, CD11b and F4/80, suggesting that TH2.52 cells carried the typical phenotype of B1 cells. Furthermore, TH2.52 B1 cells were found to express membrane-bound CD14, which plays a critical role in LPS recognition. TH2.52 B1 cells responded to a very low concentration of LPS and exhibited: (i) augmentation of DNA synthesis; (ii) activation of nuclear factor (NF)-κB; and (iii) phosphorylation of extracellular signal regulated kinase 1/2 (Erk1/2). They were markedly inhibited by anti-CD14 antibody. Therefore, the expression of membrane-bound CD14 was suggested to provide high sensitivity to LPS for TH2.52 B1 cells.

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EP - 43

JO - Journal of Endotoxin Research

JF - Journal of Endotoxin Research

IS - 1

ER -

Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14

Abstract

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