Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14

Naoki Koide, Tsuyoshi Sugiyama, Yutaka Kato, Dipshika Chakravortty, Mya Mya Mu, Tomoaki Yoshida, Teruaki Hamano, Takashi Yokochi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The role of membrane-bound CD14 in the response of mouse B1 cell lines to lipopolysaccharide (LPS) was studied. The surface profile of mouse TH2.52 B cells was positive for CD5, IgM, B220, CD11b and F4/80, suggesting that TH2.52 cells carried the typical phenotype of B1 cells. Furthermore, TH2.52 B1 cells were found to express membrane-bound CD14, which plays a critical role in LPS recognition. TH2.52 B1 cells responded to a very low concentration of LPS and exhibited: (i) augmentation of DNA synthesis; (ii) activation of nuclear factor (NF)-κB; and (iii) phosphorylation of extracellular signal regulated kinase 1/2 (Erk1/2). They were markedly inhibited by anti-CD14 antibody. Therefore, the expression of membrane-bound CD14 was suggested to provide high sensitivity to LPS for TH2.52 B1 cells.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalJournal of Endotoxin Research
Volume7
Issue number1
DOIs
Publication statusPublished - 02-2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases

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    Koide, N., Sugiyama, T., Kato, Y., Chakravortty, D., Mu, M. M., Yoshida, T., Hamano, T., & Yokochi, T. (2001). Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14. Journal of Endotoxin Research, 7(1), 39-43. https://doi.org/10.1177/09680519010070010601