MT1-MMP plays an important role in an invasive activity of malignant pleural mesothelioma cell

Takefumi Doi, Yoshimasa Maniwa, Yugo Tanaka, Shinya Tane, Shotaro Hashimoto, Yoshiharu Ohno, Wataru Nishio, Yoshihiro Nishimura, Chiho Ohbayashi, Yutaka Okita, Yoshitake Hayashi, Masahiro Yoshimura

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Malignant pleural mesothelioma (MPM) has a poor prognosis and is a treatment resistant tumor, which is increasing in frequency throughout the world. The poor prognosis is due to the aggressive local invasiveness rather than distant metastasis. In this study, we established a cell line of malignant mesothelioma from a clinical specimen and assessed the relationship between the expression of MT1-MMP and the invasion ability of that line, as well as the cultured cells of several other lines, using the simple method that we created previously. We established a cell line from a clinical specimen from a patient with malignant mesothelioma. We assessed the invasive activities of MPM cells in an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) system that enabled us to visualize cell movements during invasion. To assess the role of MT1-MMP in the invasive activity of MPM cells, we knocked down its expression by RNA interference (RNAi). The invasion assay with DL-CGH revealed that a high expression of MT1-MMP in MPM cells was associated with aggressive invasive activity. The RNAi of MT1-MMP indicated that the expression of MT1-MMP might have a crucial role in the invasiveness of MPM cells. The MT1-MMP expression in MPM cells is related to their capacity for locally aggressive spreading into the pleura and the surrounding tissues, and MT1-MMP should be a suitable molecular target for the suppression of the invasiveness of MPM.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalExperimental and Molecular Pathology
Volume90
Issue number1
DOIs
Publication statusPublished - 02-2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

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