Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum

Louis Flamand, Annie Gravel, David Boutolleau, Roberto Alvarez-Lafuente, Steve Jacobson, Mauro S. Malnati, Debra Kohn, Yi Wei Tang, Tetsushi Yoshikawa, Dharam Ablashi

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56 Citations (Scopus)

Abstract

Human herpesvirus 6 (HHV-6) is a ubiquitous virus with which infections have been associated with pathologies ranging from delayed bone marrow engraftment to a variety of neurological diseases. The lack of a standardized assay that can be used to detect and estimate HHV-6 DNA contents in various clinical specimens can lead and has led to discordant results among investigators and on the potential association of HHV-6 to diseases. To identify the most reliable and sensitive assays, an identical set of 11 coded serum samples spiked with various quantities of the HHV-6A variant (range, 4 to 400,000 genome copies/ml) was sent to eight independent laboratories around the world. Each laboratory was asked to estimate the HHV-6 DNA content by use of its own protocols and assays. Among the various assays, three TaqMan-based real-time PCR assays yielded quantities that were closest to the quantity of HHV-6 that had been spiked. To provide better homogeneity between the results from the different laboratories working on HHV-6, we propose that investigators interested in quantifying HHV-6 in clinical samples adopt one of these assays.

Original languageEnglish
Pages (from-to)2700-2706
Number of pages7
JournalJournal of clinical microbiology
Volume46
Issue number8
DOIs
Publication statusPublished - 08-2008

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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    Flamand, L., Gravel, A., Boutolleau, D., Alvarez-Lafuente, R., Jacobson, S., Malnati, M. S., Kohn, D., Tang, Y. W., Yoshikawa, T., & Ablashi, D. (2008). Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum. Journal of clinical microbiology, 46(8), 2700-2706. https://doi.org/10.1128/JCM.00370-08