Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum

Louis Flamand, Annie Gravel, David Boutolleau, Roberto Alvarez-Lafuente, Steve Jacobson, Mauro S. Malnati, Debra Kohn, Yi Wei Tang, Tetsushi Yoshikawa, Dharam Ablashi

Research output: Contribution to journalArticle

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Abstract

Human herpesvirus 6 (HHV-6) is a ubiquitous virus with which infections have been associated with pathologies ranging from delayed bone marrow engraftment to a variety of neurological diseases. The lack of a standardized assay that can be used to detect and estimate HHV-6 DNA contents in various clinical specimens can lead and has led to discordant results among investigators and on the potential association of HHV-6 to diseases. To identify the most reliable and sensitive assays, an identical set of 11 coded serum samples spiked with various quantities of the HHV-6A variant (range, 4 to 400,000 genome copies/ml) was sent to eight independent laboratories around the world. Each laboratory was asked to estimate the HHV-6 DNA content by use of its own protocols and assays. Among the various assays, three TaqMan-based real-time PCR assays yielded quantities that were closest to the quantity of HHV-6 that had been spiked. To provide better homogeneity between the results from the different laboratories working on HHV-6, we propose that investigators interested in quantifying HHV-6 in clinical samples adopt one of these assays.

Original languageEnglish
Pages (from-to)2700-2706
Number of pages7
JournalJournal of clinical microbiology
Volume46
Issue number8
DOIs
Publication statusPublished - 01-08-2008

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Human Herpesvirus 6
Polymerase Chain Reaction
DNA
Serum
Research Personnel
Real-Time Polymerase Chain Reaction
Bone Marrow
Genome
Pathology
Viruses

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Flamand, L., Gravel, A., Boutolleau, D., Alvarez-Lafuente, R., Jacobson, S., Malnati, M. S., ... Ablashi, D. (2008). Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum. Journal of clinical microbiology, 46(8), 2700-2706. https://doi.org/10.1128/JCM.00370-08
Flamand, Louis ; Gravel, Annie ; Boutolleau, David ; Alvarez-Lafuente, Roberto ; Jacobson, Steve ; Malnati, Mauro S. ; Kohn, Debra ; Tang, Yi Wei ; Yoshikawa, Tetsushi ; Ablashi, Dharam. / Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum. In: Journal of clinical microbiology. 2008 ; Vol. 46, No. 8. pp. 2700-2706.
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Flamand, L, Gravel, A, Boutolleau, D, Alvarez-Lafuente, R, Jacobson, S, Malnati, MS, Kohn, D, Tang, YW, Yoshikawa, T & Ablashi, D 2008, 'Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum', Journal of clinical microbiology, vol. 46, no. 8, pp. 2700-2706. https://doi.org/10.1128/JCM.00370-08

Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum. / Flamand, Louis; Gravel, Annie; Boutolleau, David; Alvarez-Lafuente, Roberto; Jacobson, Steve; Malnati, Mauro S.; Kohn, Debra; Tang, Yi Wei; Yoshikawa, Tetsushi; Ablashi, Dharam.

In: Journal of clinical microbiology, Vol. 46, No. 8, 01.08.2008, p. 2700-2706.

Research output: Contribution to journalArticle

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Flamand L, Gravel A, Boutolleau D, Alvarez-Lafuente R, Jacobson S, Malnati MS et al. Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum. Journal of clinical microbiology. 2008 Aug 1;46(8):2700-2706. https://doi.org/10.1128/JCM.00370-08