Mung bean (Vigna radiata (L.)) coat extract modulates macrophage functions to enhance antigen presentation: A proteomic study

Akiko Hashiguchi, Keisuke Hitachi, Wei Zhu, Jingkui Tian, Kunihiro Tsuchida, Setsuko Komatsu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The immunomodulatory effect of mung bean is mainly attributed to antioxidant properties of flavonoids; however, the precise machinery for biological effect on animal cells remains uncertain. To understand the physiological change produced by mung bean consumption, proteomic and metabolomic techniques were used. In vitro assay confirmed the importance of synergistic interaction among multiple flavonoids by IL-6 expression. Proteomic analysis detected that the abundance of 190 proteins was changed in lipopolysaccharide-stimulated RAW264.7 cells by treatment with coat extract. Pathway mapping revealed that a range of proteins were regulated including an interferon-responsive antiviral enzyme (2′-5′-oligoadenylate synthetase), antigen processing factors (immunoglobulin heavy chain-binding protein and protein disulfide-isomerase), and proteins related to proteasomal degradation. Major histocompatibility complex pathway was activated. These results suggest that mung bean consumption enhances immune response toward a Th2-promoting polarization. Biological significance This study highlighted the immunomodulation of RAW264.7 cells in response to treatment with mung bean seed coat extract, using gel-free proteomic technique. The mechanism of immunomodulation by mung bean has not been described until today except for a report which identified HMGB1 suppression as a pathway underlying the protective effect against sepsis. This study suggested that the mung bean is involved in the regulation of antigen processing and presentation, and thus shifts immune response from acute febrile illness to specific/systemic and long-lasting immunity to protect the host.

Original languageEnglish
Pages (from-to)26-37
Number of pages12
JournalJournal of Proteomics
Volume161
DOIs
Publication statusPublished - 24-05-2017

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Macrophages
Antigen Presentation
Proteomics
Flavonoids
Antigens
HMGB1 Protein
Protein Disulfide-Isomerases
Proteins
Immunomodulation
Ligases
Processing
Interferons
Machinery
Antiviral Agents
Lipopolysaccharides
Seed
Interleukin-6
Assays
Animals
Antioxidants

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Cite this

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abstract = "The immunomodulatory effect of mung bean is mainly attributed to antioxidant properties of flavonoids; however, the precise machinery for biological effect on animal cells remains uncertain. To understand the physiological change produced by mung bean consumption, proteomic and metabolomic techniques were used. In vitro assay confirmed the importance of synergistic interaction among multiple flavonoids by IL-6 expression. Proteomic analysis detected that the abundance of 190 proteins was changed in lipopolysaccharide-stimulated RAW264.7 cells by treatment with coat extract. Pathway mapping revealed that a range of proteins were regulated including an interferon-responsive antiviral enzyme (2′-5′-oligoadenylate synthetase), antigen processing factors (immunoglobulin heavy chain-binding protein and protein disulfide-isomerase), and proteins related to proteasomal degradation. Major histocompatibility complex pathway was activated. These results suggest that mung bean consumption enhances immune response toward a Th2-promoting polarization. Biological significance This study highlighted the immunomodulation of RAW264.7 cells in response to treatment with mung bean seed coat extract, using gel-free proteomic technique. The mechanism of immunomodulation by mung bean has not been described until today except for a report which identified HMGB1 suppression as a pathway underlying the protective effect against sepsis. This study suggested that the mung bean is involved in the regulation of antigen processing and presentation, and thus shifts immune response from acute febrile illness to specific/systemic and long-lasting immunity to protect the host.",
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Mung bean (Vigna radiata (L.)) coat extract modulates macrophage functions to enhance antigen presentation : A proteomic study. / Hashiguchi, Akiko; Hitachi, Keisuke; Zhu, Wei; Tian, Jingkui; Tsuchida, Kunihiro; Komatsu, Setsuko.

In: Journal of Proteomics, Vol. 161, 24.05.2017, p. 26-37.

Research output: Contribution to journalArticle

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