Mutant analyses reveal different functions of fgfr1 in medaka and zebrafish despite conserved ligand-receptor relationships

Hayato Yokoi, Atsuko Shimada, Matthias Carl, Shigeo Takashima, Daisuke Kobayashi, Takanori Narita, Tomoko Jindo, Tetsuaki Kimura, Tadao Kitagawa, Takahiro Kage, Atsushi Sawada, Kiyoshi Naruse, Shuichi Asakawa, Nobuyoshi Shimizu, Hiroshi Mitani, Akihiro Shima, Makiko Tsutsumi, Hiroshi Hori, Joachim Wittbrodt, Yumiko Saga & 3 others Yuji Ishikawa, Kazuo Araki, Hiroyuki Takeda

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Abstract

Medaka (Oryzias latipes) is a small freshwater teleost that provides an excellent developmental genetic model complementary to zebrafish. Our recent mutagenesis screening using medaka identified headfish (hdf) which is characterized by the absence of trunk and tail structures with nearly normal head including the midbrain-hindbrain boundary (MHB). Positional-candidate cloning revealed that the hdf mutation causes a functionally null form of Fgfr1. The fgfr1hdf is thus the first fgf receptor mutant in fish. Although FGF signaling has been implicated in mesoderm induction, mesoderm is induced normally in the fgfr1hdf mutant, but subsequently, mutant embryos fail to maintain the mesoderm, leading to defects in mesoderm derivatives, especially in trunk and tail. Furthermore, we found that morpholino knockdown of medaka fgf8 resulted in a phenotype identical to the fgfr1hdf mutant, suggesting that like its mouse counterpart, Fgf8 is a major ligand for Fgfr1 in medaka early embryogenesis. Intriguingly, Fgf8 and Fgfr1 in zebrafish are also suggested to form a major ligand-receptor pair, but their function is much diverged, as the zebrafish fgfr1 morphant and zebrafish fgf8 mutant acerebellar (ace) only fail to develop the MHB, but develop nearly unaffected trunk and tail. These results provide evidence that teleost fish have evolved divergent functions of Fgf8-Fgfr1 while maintaining the ligand-receptor relationships. Comparative analysis using different fish is thus invaluable for shedding light on evolutionary diversification of gene function.

Original languageEnglish
Pages (from-to)326-337
Number of pages12
JournalDevelopmental Biology
Volume304
Issue number1
DOIs
Publication statusPublished - 01-04-2007

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Oryzias
Zebrafish
Mesoderm
Ligands
Tail
Rhombencephalon
Fishes
Mesencephalon
Morpholinos
Genetic Models
Fresh Water
Mutagenesis
Embryonic Development
Organism Cloning
Embryonic Structures
Head
Phenotype
Mutation
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Yokoi, Hayato ; Shimada, Atsuko ; Carl, Matthias ; Takashima, Shigeo ; Kobayashi, Daisuke ; Narita, Takanori ; Jindo, Tomoko ; Kimura, Tetsuaki ; Kitagawa, Tadao ; Kage, Takahiro ; Sawada, Atsushi ; Naruse, Kiyoshi ; Asakawa, Shuichi ; Shimizu, Nobuyoshi ; Mitani, Hiroshi ; Shima, Akihiro ; Tsutsumi, Makiko ; Hori, Hiroshi ; Wittbrodt, Joachim ; Saga, Yumiko ; Ishikawa, Yuji ; Araki, Kazuo ; Takeda, Hiroyuki. / Mutant analyses reveal different functions of fgfr1 in medaka and zebrafish despite conserved ligand-receptor relationships. In: Developmental Biology. 2007 ; Vol. 304, No. 1. pp. 326-337.
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abstract = "Medaka (Oryzias latipes) is a small freshwater teleost that provides an excellent developmental genetic model complementary to zebrafish. Our recent mutagenesis screening using medaka identified headfish (hdf) which is characterized by the absence of trunk and tail structures with nearly normal head including the midbrain-hindbrain boundary (MHB). Positional-candidate cloning revealed that the hdf mutation causes a functionally null form of Fgfr1. The fgfr1hdf is thus the first fgf receptor mutant in fish. Although FGF signaling has been implicated in mesoderm induction, mesoderm is induced normally in the fgfr1hdf mutant, but subsequently, mutant embryos fail to maintain the mesoderm, leading to defects in mesoderm derivatives, especially in trunk and tail. Furthermore, we found that morpholino knockdown of medaka fgf8 resulted in a phenotype identical to the fgfr1hdf mutant, suggesting that like its mouse counterpart, Fgf8 is a major ligand for Fgfr1 in medaka early embryogenesis. Intriguingly, Fgf8 and Fgfr1 in zebrafish are also suggested to form a major ligand-receptor pair, but their function is much diverged, as the zebrafish fgfr1 morphant and zebrafish fgf8 mutant acerebellar (ace) only fail to develop the MHB, but develop nearly unaffected trunk and tail. These results provide evidence that teleost fish have evolved divergent functions of Fgf8-Fgfr1 while maintaining the ligand-receptor relationships. Comparative analysis using different fish is thus invaluable for shedding light on evolutionary diversification of gene function.",
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Yokoi, H, Shimada, A, Carl, M, Takashima, S, Kobayashi, D, Narita, T, Jindo, T, Kimura, T, Kitagawa, T, Kage, T, Sawada, A, Naruse, K, Asakawa, S, Shimizu, N, Mitani, H, Shima, A, Tsutsumi, M, Hori, H, Wittbrodt, J, Saga, Y, Ishikawa, Y, Araki, K & Takeda, H 2007, 'Mutant analyses reveal different functions of fgfr1 in medaka and zebrafish despite conserved ligand-receptor relationships', Developmental Biology, vol. 304, no. 1, pp. 326-337. https://doi.org/10.1016/j.ydbio.2006.12.043

Mutant analyses reveal different functions of fgfr1 in medaka and zebrafish despite conserved ligand-receptor relationships. / Yokoi, Hayato; Shimada, Atsuko; Carl, Matthias; Takashima, Shigeo; Kobayashi, Daisuke; Narita, Takanori; Jindo, Tomoko; Kimura, Tetsuaki; Kitagawa, Tadao; Kage, Takahiro; Sawada, Atsushi; Naruse, Kiyoshi; Asakawa, Shuichi; Shimizu, Nobuyoshi; Mitani, Hiroshi; Shima, Akihiro; Tsutsumi, Makiko; Hori, Hiroshi; Wittbrodt, Joachim; Saga, Yumiko; Ishikawa, Yuji; Araki, Kazuo; Takeda, Hiroyuki.

In: Developmental Biology, Vol. 304, No. 1, 01.04.2007, p. 326-337.

Research output: Contribution to journalArticle

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T1 - Mutant analyses reveal different functions of fgfr1 in medaka and zebrafish despite conserved ligand-receptor relationships

AU - Yokoi, Hayato

AU - Shimada, Atsuko

AU - Carl, Matthias

AU - Takashima, Shigeo

AU - Kobayashi, Daisuke

AU - Narita, Takanori

AU - Jindo, Tomoko

AU - Kimura, Tetsuaki

AU - Kitagawa, Tadao

AU - Kage, Takahiro

AU - Sawada, Atsushi

AU - Naruse, Kiyoshi

AU - Asakawa, Shuichi

AU - Shimizu, Nobuyoshi

AU - Mitani, Hiroshi

AU - Shima, Akihiro

AU - Tsutsumi, Makiko

AU - Hori, Hiroshi

AU - Wittbrodt, Joachim

AU - Saga, Yumiko

AU - Ishikawa, Yuji

AU - Araki, Kazuo

AU - Takeda, Hiroyuki

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Medaka (Oryzias latipes) is a small freshwater teleost that provides an excellent developmental genetic model complementary to zebrafish. Our recent mutagenesis screening using medaka identified headfish (hdf) which is characterized by the absence of trunk and tail structures with nearly normal head including the midbrain-hindbrain boundary (MHB). Positional-candidate cloning revealed that the hdf mutation causes a functionally null form of Fgfr1. The fgfr1hdf is thus the first fgf receptor mutant in fish. Although FGF signaling has been implicated in mesoderm induction, mesoderm is induced normally in the fgfr1hdf mutant, but subsequently, mutant embryos fail to maintain the mesoderm, leading to defects in mesoderm derivatives, especially in trunk and tail. Furthermore, we found that morpholino knockdown of medaka fgf8 resulted in a phenotype identical to the fgfr1hdf mutant, suggesting that like its mouse counterpart, Fgf8 is a major ligand for Fgfr1 in medaka early embryogenesis. Intriguingly, Fgf8 and Fgfr1 in zebrafish are also suggested to form a major ligand-receptor pair, but their function is much diverged, as the zebrafish fgfr1 morphant and zebrafish fgf8 mutant acerebellar (ace) only fail to develop the MHB, but develop nearly unaffected trunk and tail. These results provide evidence that teleost fish have evolved divergent functions of Fgf8-Fgfr1 while maintaining the ligand-receptor relationships. Comparative analysis using different fish is thus invaluable for shedding light on evolutionary diversification of gene function.

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