Mutation screening of GRIN2B in schizophrenia and autism spectrum disorder in a Japanese population

Yuto Takasaki, Takayoshi Koide, Chenyao Wang, Hiroki Kimura, Jingrui Xing, Itaru Kushima, Kanako Ishizuka, Daisuke Mori, Mariko Sekiguchi, Masashi Ikeda, Miki Aizawa, Naoko Tsurumaru, Yoshimi Iwayama, Akira Yoshimi, Yuko Arioka, Mami Yoshida, Hiromi Noma, Tomoko Oya-Ito, Yukako Nakamura, Shohko KunimotoBranko Aleksic, Yota Uno, Takashi Okada, Hiroshi Ujike, Jun Egawa, Hitoshi Kuwabara, Toshiyuki Someya, Takeo Yoshikawa, Nakao Iwata, Norio Ozaki

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Abstract

N-methyl-d-aspartate receptors (NMDARs) play a critical role in excitatory synaptic transmission and plasticity in the central nervous systems. Recent genetics studies in schizophrenia (SCZ) show that SCZ is susceptible to NMDARs and the NMDAR signaling complex. In autism spectrum disorder (ASD), several studies report dysregulation of NMDARs as a risk factor for ASD. To further examine the association between NMDARs and SCZ/ASD development, we conducted a mutation screening study of GRIN2B which encodes NR2B subunit of NMDARs, to identify rare mutations that potentially cause diseases, in SCZ and ASD patients (n = 574 and 152, respectively). This was followed by an association study in a large sample set of SCZ, ASD, and normal healthy controls (n = 4145, 381, and 4432, respectively). We identified five rare missense mutations through the mutation screening of GRIN2B. Although no statistically significant association between any single mutation and SCZ or ASD was found, one of its variant, K1292R, is found only in the patient group. To further examine the association between mutations in GRIN2B and SCZ/ASD development, a larger sample size and functional experiments are needed.

Original languageEnglish
Article number33311
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 12-09-2016

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Schizophrenia
Mutation
Population
Neuronal Plasticity
Missense Mutation
Autism Spectrum Disorder
Synaptic Transmission
Sample Size
aspartic acid receptor
Central Nervous System

All Science Journal Classification (ASJC) codes

  • General

Cite this

Takasaki, Y., Koide, T., Wang, C., Kimura, H., Xing, J., Kushima, I., ... Ozaki, N. (2016). Mutation screening of GRIN2B in schizophrenia and autism spectrum disorder in a Japanese population. Scientific reports, 6, [33311]. https://doi.org/10.1038/srep33311
Takasaki, Yuto ; Koide, Takayoshi ; Wang, Chenyao ; Kimura, Hiroki ; Xing, Jingrui ; Kushima, Itaru ; Ishizuka, Kanako ; Mori, Daisuke ; Sekiguchi, Mariko ; Ikeda, Masashi ; Aizawa, Miki ; Tsurumaru, Naoko ; Iwayama, Yoshimi ; Yoshimi, Akira ; Arioka, Yuko ; Yoshida, Mami ; Noma, Hiromi ; Oya-Ito, Tomoko ; Nakamura, Yukako ; Kunimoto, Shohko ; Aleksic, Branko ; Uno, Yota ; Okada, Takashi ; Ujike, Hiroshi ; Egawa, Jun ; Kuwabara, Hitoshi ; Someya, Toshiyuki ; Yoshikawa, Takeo ; Iwata, Nakao ; Ozaki, Norio. / Mutation screening of GRIN2B in schizophrenia and autism spectrum disorder in a Japanese population. In: Scientific reports. 2016 ; Vol. 6.
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abstract = "N-methyl-d-aspartate receptors (NMDARs) play a critical role in excitatory synaptic transmission and plasticity in the central nervous systems. Recent genetics studies in schizophrenia (SCZ) show that SCZ is susceptible to NMDARs and the NMDAR signaling complex. In autism spectrum disorder (ASD), several studies report dysregulation of NMDARs as a risk factor for ASD. To further examine the association between NMDARs and SCZ/ASD development, we conducted a mutation screening study of GRIN2B which encodes NR2B subunit of NMDARs, to identify rare mutations that potentially cause diseases, in SCZ and ASD patients (n = 574 and 152, respectively). This was followed by an association study in a large sample set of SCZ, ASD, and normal healthy controls (n = 4145, 381, and 4432, respectively). We identified five rare missense mutations through the mutation screening of GRIN2B. Although no statistically significant association between any single mutation and SCZ or ASD was found, one of its variant, K1292R, is found only in the patient group. To further examine the association between mutations in GRIN2B and SCZ/ASD development, a larger sample size and functional experiments are needed.",
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Takasaki, Y, Koide, T, Wang, C, Kimura, H, Xing, J, Kushima, I, Ishizuka, K, Mori, D, Sekiguchi, M, Ikeda, M, Aizawa, M, Tsurumaru, N, Iwayama, Y, Yoshimi, A, Arioka, Y, Yoshida, M, Noma, H, Oya-Ito, T, Nakamura, Y, Kunimoto, S, Aleksic, B, Uno, Y, Okada, T, Ujike, H, Egawa, J, Kuwabara, H, Someya, T, Yoshikawa, T, Iwata, N & Ozaki, N 2016, 'Mutation screening of GRIN2B in schizophrenia and autism spectrum disorder in a Japanese population', Scientific reports, vol. 6, 33311. https://doi.org/10.1038/srep33311

Mutation screening of GRIN2B in schizophrenia and autism spectrum disorder in a Japanese population. / Takasaki, Yuto; Koide, Takayoshi; Wang, Chenyao; Kimura, Hiroki; Xing, Jingrui; Kushima, Itaru; Ishizuka, Kanako; Mori, Daisuke; Sekiguchi, Mariko; Ikeda, Masashi; Aizawa, Miki; Tsurumaru, Naoko; Iwayama, Yoshimi; Yoshimi, Akira; Arioka, Yuko; Yoshida, Mami; Noma, Hiromi; Oya-Ito, Tomoko; Nakamura, Yukako; Kunimoto, Shohko; Aleksic, Branko; Uno, Yota; Okada, Takashi; Ujike, Hiroshi; Egawa, Jun; Kuwabara, Hitoshi; Someya, Toshiyuki; Yoshikawa, Takeo; Iwata, Nakao; Ozaki, Norio.

In: Scientific reports, Vol. 6, 33311, 12.09.2016.

Research output: Contribution to journalArticle

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T1 - Mutation screening of GRIN2B in schizophrenia and autism spectrum disorder in a Japanese population

AU - Takasaki, Yuto

AU - Koide, Takayoshi

AU - Wang, Chenyao

AU - Kimura, Hiroki

AU - Xing, Jingrui

AU - Kushima, Itaru

AU - Ishizuka, Kanako

AU - Mori, Daisuke

AU - Sekiguchi, Mariko

AU - Ikeda, Masashi

AU - Aizawa, Miki

AU - Tsurumaru, Naoko

AU - Iwayama, Yoshimi

AU - Yoshimi, Akira

AU - Arioka, Yuko

AU - Yoshida, Mami

AU - Noma, Hiromi

AU - Oya-Ito, Tomoko

AU - Nakamura, Yukako

AU - Kunimoto, Shohko

AU - Aleksic, Branko

AU - Uno, Yota

AU - Okada, Takashi

AU - Ujike, Hiroshi

AU - Egawa, Jun

AU - Kuwabara, Hitoshi

AU - Someya, Toshiyuki

AU - Yoshikawa, Takeo

AU - Iwata, Nakao

AU - Ozaki, Norio

PY - 2016/9/12

Y1 - 2016/9/12

N2 - N-methyl-d-aspartate receptors (NMDARs) play a critical role in excitatory synaptic transmission and plasticity in the central nervous systems. Recent genetics studies in schizophrenia (SCZ) show that SCZ is susceptible to NMDARs and the NMDAR signaling complex. In autism spectrum disorder (ASD), several studies report dysregulation of NMDARs as a risk factor for ASD. To further examine the association between NMDARs and SCZ/ASD development, we conducted a mutation screening study of GRIN2B which encodes NR2B subunit of NMDARs, to identify rare mutations that potentially cause diseases, in SCZ and ASD patients (n = 574 and 152, respectively). This was followed by an association study in a large sample set of SCZ, ASD, and normal healthy controls (n = 4145, 381, and 4432, respectively). We identified five rare missense mutations through the mutation screening of GRIN2B. Although no statistically significant association between any single mutation and SCZ or ASD was found, one of its variant, K1292R, is found only in the patient group. To further examine the association between mutations in GRIN2B and SCZ/ASD development, a larger sample size and functional experiments are needed.

AB - N-methyl-d-aspartate receptors (NMDARs) play a critical role in excitatory synaptic transmission and plasticity in the central nervous systems. Recent genetics studies in schizophrenia (SCZ) show that SCZ is susceptible to NMDARs and the NMDAR signaling complex. In autism spectrum disorder (ASD), several studies report dysregulation of NMDARs as a risk factor for ASD. To further examine the association between NMDARs and SCZ/ASD development, we conducted a mutation screening study of GRIN2B which encodes NR2B subunit of NMDARs, to identify rare mutations that potentially cause diseases, in SCZ and ASD patients (n = 574 and 152, respectively). This was followed by an association study in a large sample set of SCZ, ASD, and normal healthy controls (n = 4145, 381, and 4432, respectively). We identified five rare missense mutations through the mutation screening of GRIN2B. Although no statistically significant association between any single mutation and SCZ or ASD was found, one of its variant, K1292R, is found only in the patient group. To further examine the association between mutations in GRIN2B and SCZ/ASD development, a larger sample size and functional experiments are needed.

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