MYBPH inhibits NM IIA assembly via direct interaction with NMHC IIA and reduces cell motility

Yasuyuki Hosono, Jiro Usukura, Tomoya Yamaguchi, Kiyoshi Yanagisawa, Motoshi Suzuki, Takashi Takahashi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Actomyosin filament assembly is a critical step in tumor cell migration. We previously found that myosin binding protein H (MYBPH) is directly transactivated by the TTF-1 lineage-survival oncogene in lung adenocarcinomas and inhibits phosphorylation of the myosin regulatory light chain (RLC) of non-muscle myosin IIA (NM IIA) via direct interaction with Rho kinase 1 (ROCK1). Here, we report that MYBPH also directly interacts with an additional molecule, non-muscle myosin heavy chain IIA (NMHC IIA), which was found to occur between MYBPH and the rod portion of NMHC IIA. MYBPH inhibited NMHC IIA assembly and reduced cell motility. Conversely, siMYBPH-induced increased motility was partially, yet significantly, suppressed by blebbistatin, a non-muscle myosin II inhibitor, while more profound effects were attained by combined treatment with siROCK1 and blebbistatin. Electron microscopy observations showed well-ordered paracrystals of NMHC IIA reflecting an assembled state, which were significantly less frequently observed in the presence of MYBPH. Furthermore, an in vitro sedimentation assay showed that a greater amount of NMHC IIA was in an unassembled state in the presence of MYBPH. Interestingly, treatment with a ROCK inhibitor that impairs transition of NM IIA from an assembly-incompetent to assembly-competent state reduced the interaction between MYBPH and NMHC IIA, suggesting that MYBPH has higher affinity to assembly-competent NM IIA. These results suggest that MYBPH inhibits RLC and NMHC IIA, independent components of NM IIA, and negatively regulates actomyosin organization at 2 distinct steps, resulting in firm inhibition of NM IIA assembly.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume428
Issue number1
DOIs
Publication statusPublished - 09-11-2012
Externally publishedYes

Fingerprint

Nonmuscle Myosin Type IIA
Myosin Heavy Chains
Myosins
Cell Movement
Carrier Proteins
Actomyosin
Myosin Type II
rho-Associated Kinases
Myosin Light Chains
Phosphorylation
Oncogenes
Sedimentation
Electron microscopy
Tumors
Assays
Electron Microscopy
Cells

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hosono, Yasuyuki ; Usukura, Jiro ; Yamaguchi, Tomoya ; Yanagisawa, Kiyoshi ; Suzuki, Motoshi ; Takahashi, Takashi. / MYBPH inhibits NM IIA assembly via direct interaction with NMHC IIA and reduces cell motility. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 428, No. 1. pp. 173-178.
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MYBPH inhibits NM IIA assembly via direct interaction with NMHC IIA and reduces cell motility. / Hosono, Yasuyuki; Usukura, Jiro; Yamaguchi, Tomoya; Yanagisawa, Kiyoshi; Suzuki, Motoshi; Takahashi, Takashi.

In: Biochemical and Biophysical Research Communications, Vol. 428, No. 1, 09.11.2012, p. 173-178.

Research output: Contribution to journalArticle

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T1 - MYBPH inhibits NM IIA assembly via direct interaction with NMHC IIA and reduces cell motility

AU - Hosono, Yasuyuki

AU - Usukura, Jiro

AU - Yamaguchi, Tomoya

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AU - Suzuki, Motoshi

AU - Takahashi, Takashi

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