Myogenic Differentiation from MYOGENIN -Mutated Human iPS Cells by CRISPR/Cas9

Koki Higashioka, Noriko Koizumi, Hidetoshi Sakurai, Chie Sotozono, Takahiko Sato

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

It is well known that myogenic regulatory factors encoded by the Myod1 family of genes have pivotal roles in myogenesis, with partially overlapping functions, as demonstrated for the mouse embryo. Myogenin-mutant mice, however, exhibit severe myogenic defects without compensation by other myogenic factors. MYOGENIN might be expected to have an analogous function in human myogenic cells. To verify this hypothesis, we generated MYOGENIN-mutated human iPS cells by using CRISPR/Cas9 genome-editing technology. Our results suggest that MYOD1-independent or MYOD1-dependent mechanisms can compensate for the loss of MYOGENIN and that these mechanisms are likely to be crucial for regulating skeletal muscle differentiation and formation.

Original languageEnglish
Article number9210494
JournalStem Cells International
Volume2017
DOIs
Publication statusPublished - 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Myogenic Differentiation from MYOGENIN -Mutated Human iPS Cells by CRISPR/Cas9'. Together they form a unique fingerprint.

Cite this