Myosin binding subunit of smooth muscle myosin phosphatase at the cell-cell adhesion sites in MDCK cells

Naoyuki Inagaki, Miwako Nishizawa, Masaaki Ito, Masaki Fujioka, Takeshi Nakano, Shiho Tsujino, Kaori Matsuzawa, Kazushi Kimura, Kozo Kaibuchi, Masaki Inagaki

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We examined the intracellular localization of the myosin binding subunit (MBS) of smooth muscle myosin phosphatase. In MDCK cells in a confluent monolayer of polarized epithelial sheet, MBS was concentrated to the cell-cell adhesion sites. Double-immunofluorescence analysis with anti-MBS and anti-β-catenin antibodies showed that MBS was mainly localized at the adherens junction. Furthermore, MBS was translocated reversibly between the cytosol and the cell-cell adhesion sites during the formation and disappearance of cell-cell contacts. These data suggest that MBS may play an important role in the regulation of the cell-cell adhesion.

Original languageEnglish
Pages (from-to)552-556
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume230
Issue number3
DOIs
Publication statusPublished - 23-01-1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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