Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo

Mamoru Matsubara, Tao Jing, Kumi Kawamura, Naoshi Shimojo, Koiti Titani, Keiichiro Hashimoto, Nobuhiro Hayashi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Human immunodeficiency virus Nef is a myristoylated protein expressed early in infection by HIV. In addition to the well known down-regulation of the cell surface receptors CD4 and MHCI, Nef is able to alter T-cell signaling pathways. The ability to alter the cellular signaling pathways suggests that Nef can associate with signaling proteins. In the present report, we show that Nef can interact with calmodulin, the major intracellular receptor for calcium. Coimmunoprecipitation analyses with lysates from the NIH3T3 cell line constitutively expressing the native HIV-1 Nef protein revealed the presence of a stable Nef-calmodulin complex. When lysates from NIH3T3 cells were incubated with calmodulin-agarose beads in the presence of CaCl2 or EGTA, calcium ion drastically enhanced the interaction between Nef and calmodulin, suggesting that the binding is under the influence of Ca2+ signaling. Glutathione S-transferase-Nef fusion protein bound directly to calmodulin with high affinity. Using synthetic peptides based on the N-terminal sequence of Nef, we determined that within a 20-amino-acid N-terminal basic domain was sufficient for calmodulin binding. Furthermore, the myristoylated peptide bound to calmodulin with higher affinity than nonmyristoylated form. Thus, the N-terminal myristoylation domain of Nef plays an important role in interacting with calmodulin. This domain is highly conserved in several HIV-1 Nef variants and resembles the N-terminal domain of NAP-22/CAP23, a myristoylated calmodulin-binder. These results for the interaction between HIV Nef and calmodulin in the cells suggested that the Nef might interfere with intracellular Ca2+ signaling through calmodulin-mediated interactions in infected cells.

Original languageEnglish
Pages (from-to)494-503
Number of pages10
JournalProtein Science
Volume14
Issue number2
DOIs
Publication statusPublished - 01-02-2005

Fingerprint

Calmodulin
HIV
Cell signaling
nef Gene Products
Calcium-Sensing Receptors
Peptides
Proteins
T-cells
Egtazic Acid
Cell Surface Receptors
Glutathione Transferase
Viruses
Sepharose
HIV Infections
Binders
HIV-1
Fusion reactions
Down-Regulation
Cells
Ions

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Matsubara, Mamoru ; Jing, Tao ; Kawamura, Kumi ; Shimojo, Naoshi ; Titani, Koiti ; Hashimoto, Keiichiro ; Hayashi, Nobuhiro. / Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo. In: Protein Science. 2005 ; Vol. 14, No. 2. pp. 494-503.
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abstract = "Human immunodeficiency virus Nef is a myristoylated protein expressed early in infection by HIV. In addition to the well known down-regulation of the cell surface receptors CD4 and MHCI, Nef is able to alter T-cell signaling pathways. The ability to alter the cellular signaling pathways suggests that Nef can associate with signaling proteins. In the present report, we show that Nef can interact with calmodulin, the major intracellular receptor for calcium. Coimmunoprecipitation analyses with lysates from the NIH3T3 cell line constitutively expressing the native HIV-1 Nef protein revealed the presence of a stable Nef-calmodulin complex. When lysates from NIH3T3 cells were incubated with calmodulin-agarose beads in the presence of CaCl2 or EGTA, calcium ion drastically enhanced the interaction between Nef and calmodulin, suggesting that the binding is under the influence of Ca2+ signaling. Glutathione S-transferase-Nef fusion protein bound directly to calmodulin with high affinity. Using synthetic peptides based on the N-terminal sequence of Nef, we determined that within a 20-amino-acid N-terminal basic domain was sufficient for calmodulin binding. Furthermore, the myristoylated peptide bound to calmodulin with higher affinity than nonmyristoylated form. Thus, the N-terminal myristoylation domain of Nef plays an important role in interacting with calmodulin. This domain is highly conserved in several HIV-1 Nef variants and resembles the N-terminal domain of NAP-22/CAP23, a myristoylated calmodulin-binder. These results for the interaction between HIV Nef and calmodulin in the cells suggested that the Nef might interfere with intracellular Ca2+ signaling through calmodulin-mediated interactions in infected cells.",
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Matsubara, M, Jing, T, Kawamura, K, Shimojo, N, Titani, K, Hashimoto, K & Hayashi, N 2005, 'Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo', Protein Science, vol. 14, no. 2, pp. 494-503. https://doi.org/10.1110/ps.04969605

Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo. / Matsubara, Mamoru; Jing, Tao; Kawamura, Kumi; Shimojo, Naoshi; Titani, Koiti; Hashimoto, Keiichiro; Hayashi, Nobuhiro.

In: Protein Science, Vol. 14, No. 2, 01.02.2005, p. 494-503.

Research output: Contribution to journalArticle

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T1 - Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo

AU - Matsubara, Mamoru

AU - Jing, Tao

AU - Kawamura, Kumi

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AU - Hashimoto, Keiichiro

AU - Hayashi, Nobuhiro

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AB - Human immunodeficiency virus Nef is a myristoylated protein expressed early in infection by HIV. In addition to the well known down-regulation of the cell surface receptors CD4 and MHCI, Nef is able to alter T-cell signaling pathways. The ability to alter the cellular signaling pathways suggests that Nef can associate with signaling proteins. In the present report, we show that Nef can interact with calmodulin, the major intracellular receptor for calcium. Coimmunoprecipitation analyses with lysates from the NIH3T3 cell line constitutively expressing the native HIV-1 Nef protein revealed the presence of a stable Nef-calmodulin complex. When lysates from NIH3T3 cells were incubated with calmodulin-agarose beads in the presence of CaCl2 or EGTA, calcium ion drastically enhanced the interaction between Nef and calmodulin, suggesting that the binding is under the influence of Ca2+ signaling. Glutathione S-transferase-Nef fusion protein bound directly to calmodulin with high affinity. Using synthetic peptides based on the N-terminal sequence of Nef, we determined that within a 20-amino-acid N-terminal basic domain was sufficient for calmodulin binding. Furthermore, the myristoylated peptide bound to calmodulin with higher affinity than nonmyristoylated form. Thus, the N-terminal myristoylation domain of Nef plays an important role in interacting with calmodulin. This domain is highly conserved in several HIV-1 Nef variants and resembles the N-terminal domain of NAP-22/CAP23, a myristoylated calmodulin-binder. These results for the interaction between HIV Nef and calmodulin in the cells suggested that the Nef might interfere with intracellular Ca2+ signaling through calmodulin-mediated interactions in infected cells.

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