Myt/NZF family transcription factors regulate neuronal differentiation of P19 cells

Toshiki Kameyama, Fumio Matsushita, Yuzo Kadokawa, Tohru Marunouchi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

During mammalian central nervous system development, neural stem cells differentiate and then mature into various types of neurons. Myelin transcription factor (Myt)/neural zinc finger (NZF) family proteins were first identified as myelin proteolipid protein promoter binding factors and were shown to be involved in oligodendrocyte development. In this study, we found that Myt/NZF family molecules were expressed during neuronal differentiation in vivo and in vitro. Transient over-expression of Myt/NZF family genes could convert undifferentiated P19 cells into neurons without induction by retinoic acid (RA), and the ability of these genes to induce neuronal differentiation was comparable to that of Neurog1 and Neurod1. Additionally, we found that St18 (or NZF-3) was induced by several bHLH transcription factors. When NZF-3 and Neurog1 were co-expressed in P19 cells, the rate of neuronal differentiation was significantly increased. These data suggest not only that NZF-3 works downstream of Neurog1 but also that it plays a crucial role together with Neurog1 in neuronal differentiation.

Original languageEnglish
Pages (from-to)74-79
Number of pages6
JournalNeuroscience Letters
Volume497
Issue number2
DOIs
Publication statusPublished - 22-06-2011

Fingerprint

Zinc Fingers
Myelin Sheath
Cell Differentiation
Transcription Factors
Myelin Proteolipid Protein
Basic Helix-Loop-Helix Transcription Factors
Neurons
Neural Stem Cells
Oligodendroglia
Tretinoin
Protein Binding
Genes
Central Nervous System
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{5f83ab267b0548f1ae31fbe61f084e45,
title = "Myt/NZF family transcription factors regulate neuronal differentiation of P19 cells",
abstract = "During mammalian central nervous system development, neural stem cells differentiate and then mature into various types of neurons. Myelin transcription factor (Myt)/neural zinc finger (NZF) family proteins were first identified as myelin proteolipid protein promoter binding factors and were shown to be involved in oligodendrocyte development. In this study, we found that Myt/NZF family molecules were expressed during neuronal differentiation in vivo and in vitro. Transient over-expression of Myt/NZF family genes could convert undifferentiated P19 cells into neurons without induction by retinoic acid (RA), and the ability of these genes to induce neuronal differentiation was comparable to that of Neurog1 and Neurod1. Additionally, we found that St18 (or NZF-3) was induced by several bHLH transcription factors. When NZF-3 and Neurog1 were co-expressed in P19 cells, the rate of neuronal differentiation was significantly increased. These data suggest not only that NZF-3 works downstream of Neurog1 but also that it plays a crucial role together with Neurog1 in neuronal differentiation.",
author = "Toshiki Kameyama and Fumio Matsushita and Yuzo Kadokawa and Tohru Marunouchi",
year = "2011",
month = "6",
day = "22",
doi = "10.1016/j.neulet.2011.04.033",
language = "English",
volume = "497",
pages = "74--79",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

Myt/NZF family transcription factors regulate neuronal differentiation of P19 cells. / Kameyama, Toshiki; Matsushita, Fumio; Kadokawa, Yuzo; Marunouchi, Tohru.

In: Neuroscience Letters, Vol. 497, No. 2, 22.06.2011, p. 74-79.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Myt/NZF family transcription factors regulate neuronal differentiation of P19 cells

AU - Kameyama, Toshiki

AU - Matsushita, Fumio

AU - Kadokawa, Yuzo

AU - Marunouchi, Tohru

PY - 2011/6/22

Y1 - 2011/6/22

N2 - During mammalian central nervous system development, neural stem cells differentiate and then mature into various types of neurons. Myelin transcription factor (Myt)/neural zinc finger (NZF) family proteins were first identified as myelin proteolipid protein promoter binding factors and were shown to be involved in oligodendrocyte development. In this study, we found that Myt/NZF family molecules were expressed during neuronal differentiation in vivo and in vitro. Transient over-expression of Myt/NZF family genes could convert undifferentiated P19 cells into neurons without induction by retinoic acid (RA), and the ability of these genes to induce neuronal differentiation was comparable to that of Neurog1 and Neurod1. Additionally, we found that St18 (or NZF-3) was induced by several bHLH transcription factors. When NZF-3 and Neurog1 were co-expressed in P19 cells, the rate of neuronal differentiation was significantly increased. These data suggest not only that NZF-3 works downstream of Neurog1 but also that it plays a crucial role together with Neurog1 in neuronal differentiation.

AB - During mammalian central nervous system development, neural stem cells differentiate and then mature into various types of neurons. Myelin transcription factor (Myt)/neural zinc finger (NZF) family proteins were first identified as myelin proteolipid protein promoter binding factors and were shown to be involved in oligodendrocyte development. In this study, we found that Myt/NZF family molecules were expressed during neuronal differentiation in vivo and in vitro. Transient over-expression of Myt/NZF family genes could convert undifferentiated P19 cells into neurons without induction by retinoic acid (RA), and the ability of these genes to induce neuronal differentiation was comparable to that of Neurog1 and Neurod1. Additionally, we found that St18 (or NZF-3) was induced by several bHLH transcription factors. When NZF-3 and Neurog1 were co-expressed in P19 cells, the rate of neuronal differentiation was significantly increased. These data suggest not only that NZF-3 works downstream of Neurog1 but also that it plays a crucial role together with Neurog1 in neuronal differentiation.

UR - http://www.scopus.com/inward/record.url?scp=79956311457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956311457&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2011.04.033

DO - 10.1016/j.neulet.2011.04.033

M3 - Article

C2 - 21540077

AN - SCOPUS:79956311457

VL - 497

SP - 74

EP - 79

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2

ER -