N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx

Takayuki Murata; Satoko Iwahori; Yusuke Okuno; Hironori Nishitsuji; Yusuke Yanagi; Koichi Watashi; Takaji Wakita; Hiroshi Kimura; Kunitada Shimotohno

doi:10.3390/ijms24032265

N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx

Takayuki Murata
, Satoko Iwahori
, Yusuke Okuno
, Hironori Nishitsuji
, Yusuke Yanagi
, Koichi Watashi
, Takaji Wakita
, Hiroshi Kimura
, Kunitada Shimotohno

Virology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

N6-methyladenosine (m⁶A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m⁶A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m⁶A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m⁶A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.

Original language	English
Article number	2265
Journal	International journal of molecular sciences
Volume	24
Issue number	3
DOIs	https://doi.org/10.3390/ijms24032265
Publication status	Published - 02-2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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10.3390/ijms24032265

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title = "N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx",

abstract = "N6-methyladenosine (m6A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m6A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m6A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m6A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.",

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author = "Takayuki Murata and Satoko Iwahori and Yusuke Okuno and Hironori Nishitsuji and Yusuke Yanagi and Koichi Watashi and Takaji Wakita and Hiroshi Kimura and Kunitada Shimotohno",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

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doi = "10.3390/ijms24032265",

language = "English",

volume = "24",

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T1 - N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx

AU - Murata, Takayuki

AU - Iwahori, Satoko

AU - Okuno, Yusuke

AU - Nishitsuji, Hironori

AU - Yanagi, Yusuke

AU - Watashi, Koichi

AU - Wakita, Takaji

AU - Kimura, Hiroshi

AU - Shimotohno, Kunitada

PY - 2023/2

Y1 - 2023/2

N2 - N6-methyladenosine (m6A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m6A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m6A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m6A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.

AB - N6-methyladenosine (m6A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m6A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m6A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m6A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.

KW - HBV

KW - RNA

KW - m6A modification

KW - primary hepatocyte

UR - https://www.scopus.com/pages/publications/85147893782

UR - https://www.scopus.com/pages/publications/85147893782#tab=citedBy

U2 - 10.3390/ijms24032265

DO - 10.3390/ijms24032265

M3 - Article

C2 - 36768585

AN - SCOPUS:85147893782

SN - 1661-6596

VL - 24

JO - International journal of molecular sciences

JF - International journal of molecular sciences

IS - 3

M1 - 2265

ER -

N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx

Abstract

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