Natural Occurrence of Nuc in the Sera of Autoimmune-Prone MRL/lpr Mice

Yoshiyuki Kanai, Keiji Miura, Takemi Uehara, Mieko Amagai, Osamu Takeda, Sei Ichi Tanuma, Yoshikazu Kurosawa

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23 Citations (Scopus)

Abstract

We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.

Original languageEnglish
Pages (from-to)729-736
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume196
Issue number2
DOIs
Publication statusPublished - 29-10-1993

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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