Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5

Nobuko Oshima, Yoshitaka Iba, Ritsuko Kubota-Koketsu, Yoshizo Asano, Yoshinobu Okuno, Yoshikazu Kurosawa

Research output: Contribution to journalArticle

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Abstract

Influenza A viruses are classified into 16 subtypes according to the serotypes of hemagglutinin (HA). It is generally thought that neutralizing antibodies (Abs) are not broadly cross-reactive among HA subtypes. We examined the repertoire of neutralizing Abs against influenza viruses in humans. B lymphocytes were collected from donors by apheresis, and Ab libraries were constructed by using phage-display technology. Anti-HA clones were isolated by screening with H3N2 viruses. Their binding activity was examined, and four kinds of Abs showing broad strain specificity were identified from one donor. Two of the Abs, F045-092 and F026-427, were extensively analyzed. They neutralized not only H3N2 but also H1N1, H2N2, and H5N1 viruses, although the activities were largely varied. Flow cytometry suggested that they have the ability to bind to HA and HA1 artificially expressed on the cell surface. They show hemagglutination inhibition activity and do not compete with C179, an Ab thought to bind to the stalk region. F045-092 competes with Abs that recognize sites A and B for binding to HA. Furthermore, the serine at residue 136 in site A could be a part of the epitope. Thus, it is likely that F045-092 and F026-427 bind to a conserved epitope in the head region formed by HA1. Interestingly, while the VH1-69 gene can encode MAbs against the HA stem that are group 1 specific, F045-092 and its relatives that recognize the head region also use V H1-69. The possible epitope recognized by these clones is discussed.

Original languageEnglish
Pages (from-to)11048-11057
Number of pages10
JournalJournal of Virology
Volume85
Issue number21
DOIs
Publication statusPublished - 01-11-2011

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Hemagglutinins
hemagglutinins
Orthomyxoviridae
Influenza A virus
antibodies
Antibodies
epitopes
Epitopes
Neutralizing Antibodies
neutralizing antibodies
H2N2 Subtype Influenza A Virus
Clone Cells
Head
H3N2 Subtype Influenza A Virus
clones
H5N1 Subtype Influenza A Virus
H1N1 Subtype Influenza A Virus
Blood Component Removal
Hemagglutination
hemagglutination

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Oshima, Nobuko ; Iba, Yoshitaka ; Kubota-Koketsu, Ritsuko ; Asano, Yoshizo ; Okuno, Yoshinobu ; Kurosawa, Yoshikazu. / Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. In: Journal of Virology. 2011 ; Vol. 85, No. 21. pp. 11048-11057.
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Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. / Oshima, Nobuko; Iba, Yoshitaka; Kubota-Koketsu, Ritsuko; Asano, Yoshizo; Okuno, Yoshinobu; Kurosawa, Yoshikazu.

In: Journal of Virology, Vol. 85, No. 21, 01.11.2011, p. 11048-11057.

Research output: Contribution to journalArticle

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AU - Oshima, Nobuko

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