Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway

Hironori Inaba, Hidemasa Goto, Kousuke Kasahara, Kanako Kumamoto, Shigenobu Yonemura, Akihito Inoko, Shotaro Yamano, Hideki Wanibuchi, Dongwei He, Naoki Goshima, Tohru Kiyono, Shinji Hirotsune, Masaki Inagaki

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Primary cilia protrude from the surface of quiescent cells and disassemble at cell cycle reentry. We previously showed that ciliary reassembly is suppressed by trichoplein-mediated Aurora A activation pathway in growing cells. Here, we report that Ndel1, a well-known modulator of dynein activity, localizes at the subdistal appendage of the mother centriole, which nucleates a primary cilium. In the presence of serum, Ndel1 depletion reduces trichoplein at the mother centriole and induces unscheduled primary cilia formation, which is reverted by forced trichoplein expression or coknockdown of KCTD17 (an E3 ligase component protein for trichoplein). Serum starvation induced transient Ndel1 degradation, subsequent to the disappearance of trichoplein at the mother centriole. Forced expression of Ndel1 suppressed trichoplein degradation and axonemal microtubule extension during ciliogenesis, similar to trichoplein induction or KCTD17 knockdown. Most importantly, the proportion of ciliated and quiescent cells was increased in the kidney tubular epithelia of newborn Ndel1-hypomorphic mice. Thus, Ndel1 acts as a novel upstream regulator of the trichoplein- Aurora A pathway to inhibit primary cilia assembly.

Original languageEnglish
Pages (from-to)409-423
Number of pages15
JournalJournal of Cell Biology
Volume212
Issue number4
DOIs
Publication statusPublished - 01-01-2016

Fingerprint

Cilia
Centrioles
Dyneins
Ubiquitin-Protein Ligases
Starvation
Serum
Microtubules
Cell Cycle
Epithelium
Kidney
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Inaba, Hironori ; Goto, Hidemasa ; Kasahara, Kousuke ; Kumamoto, Kanako ; Yonemura, Shigenobu ; Inoko, Akihito ; Yamano, Shotaro ; Wanibuchi, Hideki ; He, Dongwei ; Goshima, Naoki ; Kiyono, Tohru ; Hirotsune, Shinji ; Inagaki, Masaki. / Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. In: Journal of Cell Biology. 2016 ; Vol. 212, No. 4. pp. 409-423.
@article{32c6985fc5be46139419f32a09b6e1a3,
title = "Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway",
abstract = "Primary cilia protrude from the surface of quiescent cells and disassemble at cell cycle reentry. We previously showed that ciliary reassembly is suppressed by trichoplein-mediated Aurora A activation pathway in growing cells. Here, we report that Ndel1, a well-known modulator of dynein activity, localizes at the subdistal appendage of the mother centriole, which nucleates a primary cilium. In the presence of serum, Ndel1 depletion reduces trichoplein at the mother centriole and induces unscheduled primary cilia formation, which is reverted by forced trichoplein expression or coknockdown of KCTD17 (an E3 ligase component protein for trichoplein). Serum starvation induced transient Ndel1 degradation, subsequent to the disappearance of trichoplein at the mother centriole. Forced expression of Ndel1 suppressed trichoplein degradation and axonemal microtubule extension during ciliogenesis, similar to trichoplein induction or KCTD17 knockdown. Most importantly, the proportion of ciliated and quiescent cells was increased in the kidney tubular epithelia of newborn Ndel1-hypomorphic mice. Thus, Ndel1 acts as a novel upstream regulator of the trichoplein- Aurora A pathway to inhibit primary cilia assembly.",
author = "Hironori Inaba and Hidemasa Goto and Kousuke Kasahara and Kanako Kumamoto and Shigenobu Yonemura and Akihito Inoko and Shotaro Yamano and Hideki Wanibuchi and Dongwei He and Naoki Goshima and Tohru Kiyono and Shinji Hirotsune and Masaki Inagaki",
year = "2016",
month = "1",
day = "1",
doi = "10.1083/jcb.201507046",
language = "English",
volume = "212",
pages = "409--423",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "4",

}

Inaba, H, Goto, H, Kasahara, K, Kumamoto, K, Yonemura, S, Inoko, A, Yamano, S, Wanibuchi, H, He, D, Goshima, N, Kiyono, T, Hirotsune, S & Inagaki, M 2016, 'Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway', Journal of Cell Biology, vol. 212, no. 4, pp. 409-423. https://doi.org/10.1083/jcb.201507046

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. / Inaba, Hironori; Goto, Hidemasa; Kasahara, Kousuke; Kumamoto, Kanako; Yonemura, Shigenobu; Inoko, Akihito; Yamano, Shotaro; Wanibuchi, Hideki; He, Dongwei; Goshima, Naoki; Kiyono, Tohru; Hirotsune, Shinji; Inagaki, Masaki.

In: Journal of Cell Biology, Vol. 212, No. 4, 01.01.2016, p. 409-423.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway

AU - Inaba, Hironori

AU - Goto, Hidemasa

AU - Kasahara, Kousuke

AU - Kumamoto, Kanako

AU - Yonemura, Shigenobu

AU - Inoko, Akihito

AU - Yamano, Shotaro

AU - Wanibuchi, Hideki

AU - He, Dongwei

AU - Goshima, Naoki

AU - Kiyono, Tohru

AU - Hirotsune, Shinji

AU - Inagaki, Masaki

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Primary cilia protrude from the surface of quiescent cells and disassemble at cell cycle reentry. We previously showed that ciliary reassembly is suppressed by trichoplein-mediated Aurora A activation pathway in growing cells. Here, we report that Ndel1, a well-known modulator of dynein activity, localizes at the subdistal appendage of the mother centriole, which nucleates a primary cilium. In the presence of serum, Ndel1 depletion reduces trichoplein at the mother centriole and induces unscheduled primary cilia formation, which is reverted by forced trichoplein expression or coknockdown of KCTD17 (an E3 ligase component protein for trichoplein). Serum starvation induced transient Ndel1 degradation, subsequent to the disappearance of trichoplein at the mother centriole. Forced expression of Ndel1 suppressed trichoplein degradation and axonemal microtubule extension during ciliogenesis, similar to trichoplein induction or KCTD17 knockdown. Most importantly, the proportion of ciliated and quiescent cells was increased in the kidney tubular epithelia of newborn Ndel1-hypomorphic mice. Thus, Ndel1 acts as a novel upstream regulator of the trichoplein- Aurora A pathway to inhibit primary cilia assembly.

AB - Primary cilia protrude from the surface of quiescent cells and disassemble at cell cycle reentry. We previously showed that ciliary reassembly is suppressed by trichoplein-mediated Aurora A activation pathway in growing cells. Here, we report that Ndel1, a well-known modulator of dynein activity, localizes at the subdistal appendage of the mother centriole, which nucleates a primary cilium. In the presence of serum, Ndel1 depletion reduces trichoplein at the mother centriole and induces unscheduled primary cilia formation, which is reverted by forced trichoplein expression or coknockdown of KCTD17 (an E3 ligase component protein for trichoplein). Serum starvation induced transient Ndel1 degradation, subsequent to the disappearance of trichoplein at the mother centriole. Forced expression of Ndel1 suppressed trichoplein degradation and axonemal microtubule extension during ciliogenesis, similar to trichoplein induction or KCTD17 knockdown. Most importantly, the proportion of ciliated and quiescent cells was increased in the kidney tubular epithelia of newborn Ndel1-hypomorphic mice. Thus, Ndel1 acts as a novel upstream regulator of the trichoplein- Aurora A pathway to inhibit primary cilia assembly.

UR - http://www.scopus.com/inward/record.url?scp=84959468418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959468418&partnerID=8YFLogxK

U2 - 10.1083/jcb.201507046

DO - 10.1083/jcb.201507046

M3 - Article

VL - 212

SP - 409

EP - 423

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 4

ER -