Nested introns in an intron: Evidence of multi-step splicing in a large intron of the human dystrophin pre-mRNA

Hitoshi Suzuki, Toshiki Kameyama, Kenji Ohe, Toshifumi Tsukahara, Akila Mayeda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The mechanisms by which huge human introns are spliced out precisely are poorly understood. We analyzed large intron 7 (110 199 nucleotides) generated from the human dystrophin (DMD) pre-mRNA by RT-PCR. We identified branching between the authentic 5′ splice site and the branch point; however, the sequences far from the branch site were not detectable. This RT-PCR product was resistant to exoribonuclease (RNase R) digestion, suggesting that the detected lariat intron has a closed loop structure but contains gaps in its sequence. Transient and concomitant generation of at least two branched fragments from nested introns within large intron 7 suggests internal nested splicing events before the ultimate splicing at the authentic 5′ and 3′ splice sites. Nested splicing events, which bring the authentic 5′ and 3′ splice sites into close proximity, could be one of the splicing mechanisms for the extremely large introns.

Original languageEnglish
Pages (from-to)555-561
Number of pages7
JournalFEBS Letters
Volume587
Issue number6
DOIs
Publication statusPublished - 18-03-2013

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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