Neural activity changes underlying the working memory defi cit in alpha-CaMKII heterozygous knockout mice

Naoki Matsuo, Nobuyuki Yamasaki, Koji Ohira, Keizo Takao, Keiko Toyama, Megumi Eguchi, Shun Yamaguchi, Tsuyoshi Miyakawa

Research output: Contribution to journalArticle

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Abstract

The alpha-isoform of calcium/calmodulin-dependent protein kinase II (α-CaMKII) is expressed abundantly in the forebrain and is considered to have an essential role in synaptic plasticity and cognitive function. Previously, we reported that mice heterozygous for a null mutation of α-CaMKII (α-CaMKII+/-) have profoundly dysregulated behaviors including a severe working memory deficit, which is an endophenotype of schizophrenia and other psychiatric disorders. In addition, we found that almost all the neurons in the dentate gyrus (DG) of the mutant mice failed to mature at molecular, morphological and electrophysiological levels. In the present study, to identify the brain substrates of the working memory deficit in the mutant mice, we examined the expression of the immediate early genes (IEGs), c-Fos and Arc, in the brain after a working memory version of the eight-arm radial maze test. c-Fos expression was abolished almost completely in the DG and was reduced significantly in neurons in the CA1 and CA3 areas of the hippocampus, central amygdala, and medial prefrontal cortex (mPFC). However, c-Fos expression was intact in the entorhinal and visual cortices. Immunohistochemical studies using arc promoter driven dVenus transgenic mice demonstrated that arc gene activation after the working memory task occurred in mature, but not immature neurons in the DG of wild-type mice. These results suggest crucial insights for the neural circuits underlying spatial mnemonic processing during a working memory task and suggest the involvement of α-CaMKII in the proper maturation and integration of DG neurons into these circuits.

Original languageEnglish
Article number20
JournalFrontiers in Behavioral Neuroscience
Volume3
Issue numberSEP
DOIs
Publication statusPublished - 02-09-2009

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Short-Term Memory
Knockout Mice
Dentate Gyrus
Neurons
Memory Disorders
Endophenotypes
Calcium-Calmodulin-Dependent Protein Kinases
Entorhinal Cortex
Neuronal Plasticity
Immediate-Early Genes
Brain
Visual Cortex
Prosencephalon
Prefrontal Cortex
Cognition
Transgenic Mice
Transcriptional Activation
Psychiatry
Hippocampus

All Science Journal Classification (ASJC) codes

  • Neuropsychology and Physiological Psychology
  • Cognitive Neuroscience
  • Behavioral Neuroscience

Cite this

Matsuo, Naoki ; Yamasaki, Nobuyuki ; Ohira, Koji ; Takao, Keizo ; Toyama, Keiko ; Eguchi, Megumi ; Yamaguchi, Shun ; Miyakawa, Tsuyoshi. / Neural activity changes underlying the working memory defi cit in alpha-CaMKII heterozygous knockout mice. In: Frontiers in Behavioral Neuroscience. 2009 ; Vol. 3, No. SEP.
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Neural activity changes underlying the working memory defi cit in alpha-CaMKII heterozygous knockout mice. / Matsuo, Naoki; Yamasaki, Nobuyuki; Ohira, Koji; Takao, Keizo; Toyama, Keiko; Eguchi, Megumi; Yamaguchi, Shun; Miyakawa, Tsuyoshi.

In: Frontiers in Behavioral Neuroscience, Vol. 3, No. SEP, 20, 02.09.2009.

Research output: Contribution to journalArticle

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