TY - JOUR
T1 - Neural Circuits Containing Pallidotegmental GABAergic Neurons are Involved in the Prepulse Inhibition of the Startle Reflex in Mice
AU - Takahashi, Kenji
AU - Nagai, Taku
AU - Kamei, Hiroyuki
AU - Maeda, Kenji
AU - Matsuya, Takahiro
AU - Arai, Sawako
AU - Mizoguchi, Hiroyuki
AU - Yoneda, Yukio
AU - Nabeshima, Toshitaka
AU - Takuma, Kazuhiro
AU - Yamada, Kiyofumi
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Scientific Research (No. 17790056) and for the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology of Japan, a Grant-in-Aid for Health Science Research from the Ministry of Health, Laboar and Welfare of Japan, and by a Grant from the Smoking Research Foundation. There is no conflict of interest related to this study.
PY - 2007/7/15
Y1 - 2007/7/15
N2 - Background: Prepulse inhibition (PPI) of the startle response is a measure of the inhibitory function and time-linked information processing by which a weak sensory stimulus (the prepulse) inhibits the startle response caused by a sudden intense stimulus. We attempted to clarify the neuronal circuits underlying the control of PPI of the startle reflex in mice. Methods: c-Fos immunohistochemistry was used to detect neurons activated by startle pulse and/or prepulse trials. Behavioural pharmacology and tracing studies were also conducted. Results: The lateral globus pallidus (LGP) was activated by prepulses. Activation of the caudal pontine reticular nucleus (PnC) evoked by the startle pulses was inhibited under PPI conditions. Double-immunostaining revealed that c-Fos-positive cells in the LGP following prepulse trials were GABAergic neurons. Bilateral microinjections of lidocaine into the LGP resulted in an impairment of PPI. Fluoro-gold infusion into the PnC and the pedunculopontine tegmental nucleus (PPTg) retrogradely labeled neurons in the PPTg and LGP, respectively. Microinjections of phaclofen into the PPTg significantly impaired PPI. Conclusions: These results suggest that GABAergic neurons in the LGP which project to the PPTg play a crucial role through the activation of GABAB receptors in the regulation of PPI of the startle reflex in mice.
AB - Background: Prepulse inhibition (PPI) of the startle response is a measure of the inhibitory function and time-linked information processing by which a weak sensory stimulus (the prepulse) inhibits the startle response caused by a sudden intense stimulus. We attempted to clarify the neuronal circuits underlying the control of PPI of the startle reflex in mice. Methods: c-Fos immunohistochemistry was used to detect neurons activated by startle pulse and/or prepulse trials. Behavioural pharmacology and tracing studies were also conducted. Results: The lateral globus pallidus (LGP) was activated by prepulses. Activation of the caudal pontine reticular nucleus (PnC) evoked by the startle pulses was inhibited under PPI conditions. Double-immunostaining revealed that c-Fos-positive cells in the LGP following prepulse trials were GABAergic neurons. Bilateral microinjections of lidocaine into the LGP resulted in an impairment of PPI. Fluoro-gold infusion into the PnC and the pedunculopontine tegmental nucleus (PPTg) retrogradely labeled neurons in the PPTg and LGP, respectively. Microinjections of phaclofen into the PPTg significantly impaired PPI. Conclusions: These results suggest that GABAergic neurons in the LGP which project to the PPTg play a crucial role through the activation of GABAB receptors in the regulation of PPI of the startle reflex in mice.
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U2 - 10.1016/j.biopsych.2006.06.035
DO - 10.1016/j.biopsych.2006.06.035
M3 - Article
C2 - 17027927
AN - SCOPUS:34250729222
VL - 62
SP - 148
EP - 157
JO - Biological Psychiatry
JF - Biological Psychiatry
SN - 0006-3223
IS - 2
ER -