Abstract
Background: Prepulse inhibition (PPI) of the startle response is a measure of the inhibitory function and time-linked information processing by which a weak sensory stimulus (the prepulse) inhibits the startle response caused by a sudden intense stimulus. We attempted to clarify the neuronal circuits underlying the control of PPI of the startle reflex in mice. Methods: c-Fos immunohistochemistry was used to detect neurons activated by startle pulse and/or prepulse trials. Behavioural pharmacology and tracing studies were also conducted. Results: The lateral globus pallidus (LGP) was activated by prepulses. Activation of the caudal pontine reticular nucleus (PnC) evoked by the startle pulses was inhibited under PPI conditions. Double-immunostaining revealed that c-Fos-positive cells in the LGP following prepulse trials were GABAergic neurons. Bilateral microinjections of lidocaine into the LGP resulted in an impairment of PPI. Fluoro-gold infusion into the PnC and the pedunculopontine tegmental nucleus (PPTg) retrogradely labeled neurons in the PPTg and LGP, respectively. Microinjections of phaclofen into the PPTg significantly impaired PPI. Conclusions: These results suggest that GABAergic neurons in the LGP which project to the PPTg play a crucial role through the activation of GABAB receptors in the regulation of PPI of the startle reflex in mice.
| Original language | English |
|---|---|
| Pages (from-to) | 148-157 |
| Number of pages | 10 |
| Journal | Biological Psychiatry |
| Volume | 62 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 15-07-2007 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biological Psychiatry
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Neural Circuits Containing Pallidotegmental GABAergic Neurons are Involved in the Prepulse Inhibition of the Startle Reflex in Mice. / Takahashi, Kenji; Nagai, Taku; Kamei, Hiroyuki; Maeda, Kenji; Matsuya, Takahiro; Arai, Sawako; Mizoguchi, Hiroyuki; Yoneda, Yukio; Nabeshima, Toshitaka; Takuma, Kazuhiro; Yamada, Kiyofumi.
In: Biological Psychiatry, Vol. 62, No. 2, 15.07.2007, p. 148-157.Research output: Contribution to journal › Article
TY - JOUR
T1 - Neural Circuits Containing Pallidotegmental GABAergic Neurons are Involved in the Prepulse Inhibition of the Startle Reflex in Mice
AU - Takahashi, Kenji
AU - Nagai, Taku
AU - Kamei, Hiroyuki
AU - Maeda, Kenji
AU - Matsuya, Takahiro
AU - Arai, Sawako
AU - Mizoguchi, Hiroyuki
AU - Yoneda, Yukio
AU - Nabeshima, Toshitaka
AU - Takuma, Kazuhiro
AU - Yamada, Kiyofumi
PY - 2007/7/15
Y1 - 2007/7/15
N2 - Background: Prepulse inhibition (PPI) of the startle response is a measure of the inhibitory function and time-linked information processing by which a weak sensory stimulus (the prepulse) inhibits the startle response caused by a sudden intense stimulus. We attempted to clarify the neuronal circuits underlying the control of PPI of the startle reflex in mice. Methods: c-Fos immunohistochemistry was used to detect neurons activated by startle pulse and/or prepulse trials. Behavioural pharmacology and tracing studies were also conducted. Results: The lateral globus pallidus (LGP) was activated by prepulses. Activation of the caudal pontine reticular nucleus (PnC) evoked by the startle pulses was inhibited under PPI conditions. Double-immunostaining revealed that c-Fos-positive cells in the LGP following prepulse trials were GABAergic neurons. Bilateral microinjections of lidocaine into the LGP resulted in an impairment of PPI. Fluoro-gold infusion into the PnC and the pedunculopontine tegmental nucleus (PPTg) retrogradely labeled neurons in the PPTg and LGP, respectively. Microinjections of phaclofen into the PPTg significantly impaired PPI. Conclusions: These results suggest that GABAergic neurons in the LGP which project to the PPTg play a crucial role through the activation of GABAB receptors in the regulation of PPI of the startle reflex in mice.
AB - Background: Prepulse inhibition (PPI) of the startle response is a measure of the inhibitory function and time-linked information processing by which a weak sensory stimulus (the prepulse) inhibits the startle response caused by a sudden intense stimulus. We attempted to clarify the neuronal circuits underlying the control of PPI of the startle reflex in mice. Methods: c-Fos immunohistochemistry was used to detect neurons activated by startle pulse and/or prepulse trials. Behavioural pharmacology and tracing studies were also conducted. Results: The lateral globus pallidus (LGP) was activated by prepulses. Activation of the caudal pontine reticular nucleus (PnC) evoked by the startle pulses was inhibited under PPI conditions. Double-immunostaining revealed that c-Fos-positive cells in the LGP following prepulse trials were GABAergic neurons. Bilateral microinjections of lidocaine into the LGP resulted in an impairment of PPI. Fluoro-gold infusion into the PnC and the pedunculopontine tegmental nucleus (PPTg) retrogradely labeled neurons in the PPTg and LGP, respectively. Microinjections of phaclofen into the PPTg significantly impaired PPI. Conclusions: These results suggest that GABAergic neurons in the LGP which project to the PPTg play a crucial role through the activation of GABAB receptors in the regulation of PPI of the startle reflex in mice.
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UR - http://www.scopus.com/inward/citedby.url?scp=34250729222&partnerID=8YFLogxK
U2 - 10.1016/j.biopsych.2006.06.035
DO - 10.1016/j.biopsych.2006.06.035
M3 - Article
C2 - 17027927
AN - SCOPUS:34250729222
VL - 62
SP - 148
EP - 157
JO - Biological Psychiatry
JF - Biological Psychiatry
SN - 0006-3223
IS - 2
ER -