Neurexin-1, a presynaptic adhesion molecule, localizes at the slit diaphragm of the glomerular podocytes in kidneys

Akira Saito, Naoko Miyauchi, Taeko Hashimoto, Tamaki Karasawa, Gi Dong Han, Mutsumi Kayaba, Tomoyuki Sumi, Masayuki Tomita, Yohei Ikezumi, Kenji Suzuki, Yasushi Koitabashi, Fujio Shimizu, Hiroshi Kawachi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The slit diaphragm connecting the adjacent foot processes of glomerular epithelial cells (podocytes) is the final barrier of the glomerular capillary wall and serves to prevent proteinuria. Podocytes are understood to be terminally differentiated cells and share some common features with neurons. Neurexin is a presynaptic adhesion molecule that plays a role in synaptic differentiation. Although neurexin has been understood to be specifically expressed in neuronal tissues, we found that neurexin was expressed in several organs. Several forms of splice variants of neurexin-1α were detected in the cerebrum, but only one form of neurexin-1α was detected in glomeruli. Immunohistochemical study showed that neurexin restrictedly expressed in the podocytes in kidneys. Dual-labeling analyses showed that neurexin was colocalized with CD2AP, an intracellular component of the slit diaphragm. Immunoprecipitation assay using glomerular lysate showed that neurexin interacted with CD2AP and CASK. These observations indicated that neurexin localized at the slit diaphragm area. The staining intensity of neurexin in podocytes was clearly lowered, and their staining pattern shifted to a more discontinuous patchy pattern in the disease models showing severe proteinuria. The expression and localization of neurexin in these models altered more clearly and rapidly than that of other slit diaphragm components. We propose that neurexin is available as an early diagnostic marker to detect podocyte injury. Neurexin coincided with nephrin, a key molecule of the slit diaphragm detected in a presumptive podocyte of the developing glomeruli and in the glomeruli for which the slit diaphragm is repairing injury. These observations suggest that neurexin is involved in the formation of the slit diaphragm and the maintenance of its function.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume300
Issue number2
DOIs
Publication statusPublished - 01-02-2011

Fingerprint

Podocytes
Diaphragm
Kidney
Proteinuria
Staining and Labeling
Wounds and Injuries
Cerebrum
Immunoprecipitation
Foot
Epithelial Cells
Maintenance
Neurons

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Saito, Akira ; Miyauchi, Naoko ; Hashimoto, Taeko ; Karasawa, Tamaki ; Han, Gi Dong ; Kayaba, Mutsumi ; Sumi, Tomoyuki ; Tomita, Masayuki ; Ikezumi, Yohei ; Suzuki, Kenji ; Koitabashi, Yasushi ; Shimizu, Fujio ; Kawachi, Hiroshi. / Neurexin-1, a presynaptic adhesion molecule, localizes at the slit diaphragm of the glomerular podocytes in kidneys. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2011 ; Vol. 300, No. 2.
@article{d2551d38b36747f18c6904af560d6d1b,
title = "Neurexin-1, a presynaptic adhesion molecule, localizes at the slit diaphragm of the glomerular podocytes in kidneys",
abstract = "The slit diaphragm connecting the adjacent foot processes of glomerular epithelial cells (podocytes) is the final barrier of the glomerular capillary wall and serves to prevent proteinuria. Podocytes are understood to be terminally differentiated cells and share some common features with neurons. Neurexin is a presynaptic adhesion molecule that plays a role in synaptic differentiation. Although neurexin has been understood to be specifically expressed in neuronal tissues, we found that neurexin was expressed in several organs. Several forms of splice variants of neurexin-1α were detected in the cerebrum, but only one form of neurexin-1α was detected in glomeruli. Immunohistochemical study showed that neurexin restrictedly expressed in the podocytes in kidneys. Dual-labeling analyses showed that neurexin was colocalized with CD2AP, an intracellular component of the slit diaphragm. Immunoprecipitation assay using glomerular lysate showed that neurexin interacted with CD2AP and CASK. These observations indicated that neurexin localized at the slit diaphragm area. The staining intensity of neurexin in podocytes was clearly lowered, and their staining pattern shifted to a more discontinuous patchy pattern in the disease models showing severe proteinuria. The expression and localization of neurexin in these models altered more clearly and rapidly than that of other slit diaphragm components. We propose that neurexin is available as an early diagnostic marker to detect podocyte injury. Neurexin coincided with nephrin, a key molecule of the slit diaphragm detected in a presumptive podocyte of the developing glomeruli and in the glomeruli for which the slit diaphragm is repairing injury. These observations suggest that neurexin is involved in the formation of the slit diaphragm and the maintenance of its function.",
author = "Akira Saito and Naoko Miyauchi and Taeko Hashimoto and Tamaki Karasawa and Han, {Gi Dong} and Mutsumi Kayaba and Tomoyuki Sumi and Masayuki Tomita and Yohei Ikezumi and Kenji Suzuki and Yasushi Koitabashi and Fujio Shimizu and Hiroshi Kawachi",
year = "2011",
month = "2",
day = "1",
doi = "10.1152/ajpregu.00640.2009",
language = "English",
volume = "300",
journal = "American Journal of Physiology - Regulatory Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "2",

}

Saito, A, Miyauchi, N, Hashimoto, T, Karasawa, T, Han, GD, Kayaba, M, Sumi, T, Tomita, M, Ikezumi, Y, Suzuki, K, Koitabashi, Y, Shimizu, F & Kawachi, H 2011, 'Neurexin-1, a presynaptic adhesion molecule, localizes at the slit diaphragm of the glomerular podocytes in kidneys', American Journal of Physiology - Regulatory Integrative and Comparative Physiology, vol. 300, no. 2. https://doi.org/10.1152/ajpregu.00640.2009

Neurexin-1, a presynaptic adhesion molecule, localizes at the slit diaphragm of the glomerular podocytes in kidneys. / Saito, Akira; Miyauchi, Naoko; Hashimoto, Taeko; Karasawa, Tamaki; Han, Gi Dong; Kayaba, Mutsumi; Sumi, Tomoyuki; Tomita, Masayuki; Ikezumi, Yohei; Suzuki, Kenji; Koitabashi, Yasushi; Shimizu, Fujio; Kawachi, Hiroshi.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 300, No. 2, 01.02.2011.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Neurexin-1, a presynaptic adhesion molecule, localizes at the slit diaphragm of the glomerular podocytes in kidneys

AU - Saito, Akira

AU - Miyauchi, Naoko

AU - Hashimoto, Taeko

AU - Karasawa, Tamaki

AU - Han, Gi Dong

AU - Kayaba, Mutsumi

AU - Sumi, Tomoyuki

AU - Tomita, Masayuki

AU - Ikezumi, Yohei

AU - Suzuki, Kenji

AU - Koitabashi, Yasushi

AU - Shimizu, Fujio

AU - Kawachi, Hiroshi

PY - 2011/2/1

Y1 - 2011/2/1

N2 - The slit diaphragm connecting the adjacent foot processes of glomerular epithelial cells (podocytes) is the final barrier of the glomerular capillary wall and serves to prevent proteinuria. Podocytes are understood to be terminally differentiated cells and share some common features with neurons. Neurexin is a presynaptic adhesion molecule that plays a role in synaptic differentiation. Although neurexin has been understood to be specifically expressed in neuronal tissues, we found that neurexin was expressed in several organs. Several forms of splice variants of neurexin-1α were detected in the cerebrum, but only one form of neurexin-1α was detected in glomeruli. Immunohistochemical study showed that neurexin restrictedly expressed in the podocytes in kidneys. Dual-labeling analyses showed that neurexin was colocalized with CD2AP, an intracellular component of the slit diaphragm. Immunoprecipitation assay using glomerular lysate showed that neurexin interacted with CD2AP and CASK. These observations indicated that neurexin localized at the slit diaphragm area. The staining intensity of neurexin in podocytes was clearly lowered, and their staining pattern shifted to a more discontinuous patchy pattern in the disease models showing severe proteinuria. The expression and localization of neurexin in these models altered more clearly and rapidly than that of other slit diaphragm components. We propose that neurexin is available as an early diagnostic marker to detect podocyte injury. Neurexin coincided with nephrin, a key molecule of the slit diaphragm detected in a presumptive podocyte of the developing glomeruli and in the glomeruli for which the slit diaphragm is repairing injury. These observations suggest that neurexin is involved in the formation of the slit diaphragm and the maintenance of its function.

AB - The slit diaphragm connecting the adjacent foot processes of glomerular epithelial cells (podocytes) is the final barrier of the glomerular capillary wall and serves to prevent proteinuria. Podocytes are understood to be terminally differentiated cells and share some common features with neurons. Neurexin is a presynaptic adhesion molecule that plays a role in synaptic differentiation. Although neurexin has been understood to be specifically expressed in neuronal tissues, we found that neurexin was expressed in several organs. Several forms of splice variants of neurexin-1α were detected in the cerebrum, but only one form of neurexin-1α was detected in glomeruli. Immunohistochemical study showed that neurexin restrictedly expressed in the podocytes in kidneys. Dual-labeling analyses showed that neurexin was colocalized with CD2AP, an intracellular component of the slit diaphragm. Immunoprecipitation assay using glomerular lysate showed that neurexin interacted with CD2AP and CASK. These observations indicated that neurexin localized at the slit diaphragm area. The staining intensity of neurexin in podocytes was clearly lowered, and their staining pattern shifted to a more discontinuous patchy pattern in the disease models showing severe proteinuria. The expression and localization of neurexin in these models altered more clearly and rapidly than that of other slit diaphragm components. We propose that neurexin is available as an early diagnostic marker to detect podocyte injury. Neurexin coincided with nephrin, a key molecule of the slit diaphragm detected in a presumptive podocyte of the developing glomeruli and in the glomeruli for which the slit diaphragm is repairing injury. These observations suggest that neurexin is involved in the formation of the slit diaphragm and the maintenance of its function.

UR - http://www.scopus.com/inward/record.url?scp=79551533801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551533801&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.00640.2009

DO - 10.1152/ajpregu.00640.2009

M3 - Article

C2 - 21048075

AN - SCOPUS:79551533801

VL - 300

JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 2

ER -