Neurogranin null mutant mice display performance deficits on spatial learning tasks with anxiety related components

Tsuyoshi Miyakawa, Edom Yared, Jhang Ho Pak, Freesia L. Huang, Kuo Ping Huang, Jacqueline N. Crawley

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Neurogranin/RC3 is a protein that binds calmodulin and serves as a substrate for protein kinase C. Neuronally distributed in the hippocampus and forebrain, neurogranin is highly expressed in dendritic spines of hippocampal pyramidal cells, implicating this protein in long-term potentiation and in learning and memory processes. Null mutation of the neurogranin gene Ng generated viable knockout mice for analysis of the behavioral phenotype resulting from the absence of neurogranin protein. Ng -/- mice were normal on measures of general health, neurological reflexes, sensory abilities, and motor functions, as compared to wild type littermate controls. On the Morris water task, Ng -/- mice failed to reach acquisition criterion on the hidden platform test and did not show selective search on the probe trial. In the Barnes circular maze, another test for spatial navigation learning, Ng -/- mice showed impairments on some components of transfer, but normal performance on time spent around the target hole. Abnormal and idiosyncratic behaviors were detected, that appeared to represent an anxiogenic phenotype in Ng -/- mice, as measured in the light ↔ dark exploration test and the open field center time parameter. These findings of apparent deficits in spatial learning and anxiety-like tendencies in Ng -/- support a role for neurogranin in the hippocampally-mediated interaction between stress and performance.

Original languageEnglish
Pages (from-to)763-775
Number of pages13
JournalHippocampus
Volume11
Issue number6
DOIs
Publication statusPublished - 01-12-2001
Externally publishedYes

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Neurogranin
Anxiety
Phenotype
Dendritic Spines
Proteins
Aptitude
Long-Term Potentiation
Pyramidal Cells
Calmodulin
Prosencephalon
Knockout Mice
Protein Kinase C
Reflex
Hippocampus
Spatial Learning
Learning
Light
Mutation
Water
Health

All Science Journal Classification (ASJC) codes

  • Cognitive Neuroscience

Cite this

Miyakawa, Tsuyoshi ; Yared, Edom ; Pak, Jhang Ho ; Huang, Freesia L. ; Huang, Kuo Ping ; Crawley, Jacqueline N. / Neurogranin null mutant mice display performance deficits on spatial learning tasks with anxiety related components. In: Hippocampus. 2001 ; Vol. 11, No. 6. pp. 763-775.
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Neurogranin null mutant mice display performance deficits on spatial learning tasks with anxiety related components. / Miyakawa, Tsuyoshi; Yared, Edom; Pak, Jhang Ho; Huang, Freesia L.; Huang, Kuo Ping; Crawley, Jacqueline N.

In: Hippocampus, Vol. 11, No. 6, 01.12.2001, p. 763-775.

Research output: Contribution to journalArticle

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