Neuronal guidance protein netrin-1 induces differentiation in human embryonal carcinoma cells

Mario Mancino, Claudia Esposito, Kazuhide Watanabe, Tadahiro Nagaoka, Monica Gonzales, Caterina Bianco, Nicola Normanno, David S. Salomon, Luigi Strizzi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Pluripotent cells within embryonal carcinoma (EC) can differentiate in vivo or in vitro on treatment with specific agents. Differentiating EC cells express lower levels of stem cell-related genes, such as Cripto-1. We show that migration of human EC cells (NTEBA/2 and NCCIT) can be reduced following treatment with the guidance molecule Netrin-1. Moreover, Netrin-1 treatment increased the levels of ß-III tubulin, glial filament acidic protein, Nestin, and 7-aminobutyric acid and reduced the expressions of Cripto-1, Nanog, and Oct4 in EC cells. These Netrin-1-induced effects in the EC cells were mediated via binding of Netrin-1 to the Neogenin receptor and activation of SHP-2, resulting in increased levels of inactive phosphorylated c-src(YS27). These results suggest that Netrin-1 can induce neuroectodermal-like differentiation of human EC cells by affecting c-src signaling via SHP-2 activation and regulation of Nanog, Oct4, and Cripto-1 expressions.

Original languageEnglish
Pages (from-to)1717-1721
Number of pages5
JournalCancer Research
Volume69
Issue number5
DOIs
Publication statusPublished - 01-03-2009
Externally publishedYes

Fingerprint

Embryonal Carcinoma Stem Cells
Proteins
Aminobutyrates
Nestin
Tubulin
Neuroglia
Axon Guidance
netrin-1
Stem Cells
Genes

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

Cite this

Mancino, M., Esposito, C., Watanabe, K., Nagaoka, T., Gonzales, M., Bianco, C., ... Strizzi, L. (2009). Neuronal guidance protein netrin-1 induces differentiation in human embryonal carcinoma cells. Cancer Research, 69(5), 1717-1721. https://doi.org/10.1158/0008-5472.CAN-08-2985
Mancino, Mario ; Esposito, Claudia ; Watanabe, Kazuhide ; Nagaoka, Tadahiro ; Gonzales, Monica ; Bianco, Caterina ; Normanno, Nicola ; Salomon, David S. ; Strizzi, Luigi. / Neuronal guidance protein netrin-1 induces differentiation in human embryonal carcinoma cells. In: Cancer Research. 2009 ; Vol. 69, No. 5. pp. 1717-1721.
@article{285b1656711b44ae8282526ed72b10e3,
title = "Neuronal guidance protein netrin-1 induces differentiation in human embryonal carcinoma cells",
abstract = "Pluripotent cells within embryonal carcinoma (EC) can differentiate in vivo or in vitro on treatment with specific agents. Differentiating EC cells express lower levels of stem cell-related genes, such as Cripto-1. We show that migration of human EC cells (NTEBA/2 and NCCIT) can be reduced following treatment with the guidance molecule Netrin-1. Moreover, Netrin-1 treatment increased the levels of {\ss}-III tubulin, glial filament acidic protein, Nestin, and 7-aminobutyric acid and reduced the expressions of Cripto-1, Nanog, and Oct4 in EC cells. These Netrin-1-induced effects in the EC cells were mediated via binding of Netrin-1 to the Neogenin receptor and activation of SHP-2, resulting in increased levels of inactive phosphorylated c-src(YS27). These results suggest that Netrin-1 can induce neuroectodermal-like differentiation of human EC cells by affecting c-src signaling via SHP-2 activation and regulation of Nanog, Oct4, and Cripto-1 expressions.",
author = "Mario Mancino and Claudia Esposito and Kazuhide Watanabe and Tadahiro Nagaoka and Monica Gonzales and Caterina Bianco and Nicola Normanno and Salomon, {David S.} and Luigi Strizzi",
year = "2009",
month = "3",
day = "1",
doi = "10.1158/0008-5472.CAN-08-2985",
language = "English",
volume = "69",
pages = "1717--1721",
journal = "Cancer Research",
issn = "0008-5472",
number = "5",

}

Mancino, M, Esposito, C, Watanabe, K, Nagaoka, T, Gonzales, M, Bianco, C, Normanno, N, Salomon, DS & Strizzi, L 2009, 'Neuronal guidance protein netrin-1 induces differentiation in human embryonal carcinoma cells', Cancer Research, vol. 69, no. 5, pp. 1717-1721. https://doi.org/10.1158/0008-5472.CAN-08-2985

Neuronal guidance protein netrin-1 induces differentiation in human embryonal carcinoma cells. / Mancino, Mario; Esposito, Claudia; Watanabe, Kazuhide; Nagaoka, Tadahiro; Gonzales, Monica; Bianco, Caterina; Normanno, Nicola; Salomon, David S.; Strizzi, Luigi.

In: Cancer Research, Vol. 69, No. 5, 01.03.2009, p. 1717-1721.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Neuronal guidance protein netrin-1 induces differentiation in human embryonal carcinoma cells

AU - Mancino, Mario

AU - Esposito, Claudia

AU - Watanabe, Kazuhide

AU - Nagaoka, Tadahiro

AU - Gonzales, Monica

AU - Bianco, Caterina

AU - Normanno, Nicola

AU - Salomon, David S.

AU - Strizzi, Luigi

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Pluripotent cells within embryonal carcinoma (EC) can differentiate in vivo or in vitro on treatment with specific agents. Differentiating EC cells express lower levels of stem cell-related genes, such as Cripto-1. We show that migration of human EC cells (NTEBA/2 and NCCIT) can be reduced following treatment with the guidance molecule Netrin-1. Moreover, Netrin-1 treatment increased the levels of ß-III tubulin, glial filament acidic protein, Nestin, and 7-aminobutyric acid and reduced the expressions of Cripto-1, Nanog, and Oct4 in EC cells. These Netrin-1-induced effects in the EC cells were mediated via binding of Netrin-1 to the Neogenin receptor and activation of SHP-2, resulting in increased levels of inactive phosphorylated c-src(YS27). These results suggest that Netrin-1 can induce neuroectodermal-like differentiation of human EC cells by affecting c-src signaling via SHP-2 activation and regulation of Nanog, Oct4, and Cripto-1 expressions.

AB - Pluripotent cells within embryonal carcinoma (EC) can differentiate in vivo or in vitro on treatment with specific agents. Differentiating EC cells express lower levels of stem cell-related genes, such as Cripto-1. We show that migration of human EC cells (NTEBA/2 and NCCIT) can be reduced following treatment with the guidance molecule Netrin-1. Moreover, Netrin-1 treatment increased the levels of ß-III tubulin, glial filament acidic protein, Nestin, and 7-aminobutyric acid and reduced the expressions of Cripto-1, Nanog, and Oct4 in EC cells. These Netrin-1-induced effects in the EC cells were mediated via binding of Netrin-1 to the Neogenin receptor and activation of SHP-2, resulting in increased levels of inactive phosphorylated c-src(YS27). These results suggest that Netrin-1 can induce neuroectodermal-like differentiation of human EC cells by affecting c-src signaling via SHP-2 activation and regulation of Nanog, Oct4, and Cripto-1 expressions.

UR - http://www.scopus.com/inward/record.url?scp=62449298017&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62449298017&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-08-2985

DO - 10.1158/0008-5472.CAN-08-2985

M3 - Article

VL - 69

SP - 1717

EP - 1721

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 5

ER -