Neuronal roles of the integrin-associated protein (IAP/ CD47) in developing cortical neurons

Tadahiro Numakawa, Tetsuya Ishimoto, Shingo Suzuki, Yumiko Numakawa, Naoki Adachi, Tomoya Matsumoto, Daisaku Yokomaku, Hisatsugu Koshimizu, Kazuhiro E. Fujimori, Ryota Hashimoto, Takahisa Taguchi, Hiroshi Kunugi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Little is known about the role of the integrin-associated protein (IAP, or CD47) in neuronal development and its function in the central nervous system. We investigated neuronal responses in IAP-overexpressing cortical neurons using a virus-gene transfer system. We found that dendritic outgrowth was significantly enhanced in IAP (form 4)-transfected neurons. Furthermore, synaptic proteins including synaptotagmin, syntaxin, synapsin I, and SNAP25 (25-kDa synaptosomal associated protein) were up-regulated. In accordance with this finding, the release of the excitatory transmitter glutamate and the frequencies of Ca 2+ oscillations (glutamate-mediated synaptic transmission) were increased. Interestingly, the overexpression of IAP activated mitogen-activated protein kinase (MAPK), and this activation was required for the IAP-dependent biological effects. After down-regulation of the endogenous IAP by small interfering RNA, MAPK activity, synaptic protein levels, and glutamate release decreased. These observations suggest that the IAP plays important roles in dendritic outgrowth and synaptic transmission in developing cortical neurons through the activation of MAPK.

Original languageEnglish
Pages (from-to)43245-43253
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number41
DOIs
Publication statusPublished - 08-10-2004

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Mitogen-Activated Protein Kinases
Integrins
Neurons
Glutamic Acid
Synaptic Transmission
Synaptosomal-Associated Protein 25
Synaptotagmins
Synapsins
eIF-2 Kinase
Qa-SNARE Proteins
Proteins
Chemical activation
Gene transfer
Small Interfering RNA
Neurology
Down-Regulation
Central Nervous System
Viruses
Transmitters
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Numakawa, T., Ishimoto, T., Suzuki, S., Numakawa, Y., Adachi, N., Matsumoto, T., ... Kunugi, H. (2004). Neuronal roles of the integrin-associated protein (IAP/ CD47) in developing cortical neurons. Journal of Biological Chemistry, 279(41), 43245-43253. https://doi.org/10.1074/jbc.M406733200
Numakawa, Tadahiro ; Ishimoto, Tetsuya ; Suzuki, Shingo ; Numakawa, Yumiko ; Adachi, Naoki ; Matsumoto, Tomoya ; Yokomaku, Daisaku ; Koshimizu, Hisatsugu ; Fujimori, Kazuhiro E. ; Hashimoto, Ryota ; Taguchi, Takahisa ; Kunugi, Hiroshi. / Neuronal roles of the integrin-associated protein (IAP/ CD47) in developing cortical neurons. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 41. pp. 43245-43253.
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abstract = "Little is known about the role of the integrin-associated protein (IAP, or CD47) in neuronal development and its function in the central nervous system. We investigated neuronal responses in IAP-overexpressing cortical neurons using a virus-gene transfer system. We found that dendritic outgrowth was significantly enhanced in IAP (form 4)-transfected neurons. Furthermore, synaptic proteins including synaptotagmin, syntaxin, synapsin I, and SNAP25 (25-kDa synaptosomal associated protein) were up-regulated. In accordance with this finding, the release of the excitatory transmitter glutamate and the frequencies of Ca 2+ oscillations (glutamate-mediated synaptic transmission) were increased. Interestingly, the overexpression of IAP activated mitogen-activated protein kinase (MAPK), and this activation was required for the IAP-dependent biological effects. After down-regulation of the endogenous IAP by small interfering RNA, MAPK activity, synaptic protein levels, and glutamate release decreased. These observations suggest that the IAP plays important roles in dendritic outgrowth and synaptic transmission in developing cortical neurons through the activation of MAPK.",
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Numakawa, T, Ishimoto, T, Suzuki, S, Numakawa, Y, Adachi, N, Matsumoto, T, Yokomaku, D, Koshimizu, H, Fujimori, KE, Hashimoto, R, Taguchi, T & Kunugi, H 2004, 'Neuronal roles of the integrin-associated protein (IAP/ CD47) in developing cortical neurons', Journal of Biological Chemistry, vol. 279, no. 41, pp. 43245-43253. https://doi.org/10.1074/jbc.M406733200

Neuronal roles of the integrin-associated protein (IAP/ CD47) in developing cortical neurons. / Numakawa, Tadahiro; Ishimoto, Tetsuya; Suzuki, Shingo; Numakawa, Yumiko; Adachi, Naoki; Matsumoto, Tomoya; Yokomaku, Daisaku; Koshimizu, Hisatsugu; Fujimori, Kazuhiro E.; Hashimoto, Ryota; Taguchi, Takahisa; Kunugi, Hiroshi.

In: Journal of Biological Chemistry, Vol. 279, No. 41, 08.10.2004, p. 43245-43253.

Research output: Contribution to journalArticle

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T1 - Neuronal roles of the integrin-associated protein (IAP/ CD47) in developing cortical neurons

AU - Numakawa, Tadahiro

AU - Ishimoto, Tetsuya

AU - Suzuki, Shingo

AU - Numakawa, Yumiko

AU - Adachi, Naoki

AU - Matsumoto, Tomoya

AU - Yokomaku, Daisaku

AU - Koshimizu, Hisatsugu

AU - Fujimori, Kazuhiro E.

AU - Hashimoto, Ryota

AU - Taguchi, Takahisa

AU - Kunugi, Hiroshi

PY - 2004/10/8

Y1 - 2004/10/8

N2 - Little is known about the role of the integrin-associated protein (IAP, or CD47) in neuronal development and its function in the central nervous system. We investigated neuronal responses in IAP-overexpressing cortical neurons using a virus-gene transfer system. We found that dendritic outgrowth was significantly enhanced in IAP (form 4)-transfected neurons. Furthermore, synaptic proteins including synaptotagmin, syntaxin, synapsin I, and SNAP25 (25-kDa synaptosomal associated protein) were up-regulated. In accordance with this finding, the release of the excitatory transmitter glutamate and the frequencies of Ca 2+ oscillations (glutamate-mediated synaptic transmission) were increased. Interestingly, the overexpression of IAP activated mitogen-activated protein kinase (MAPK), and this activation was required for the IAP-dependent biological effects. After down-regulation of the endogenous IAP by small interfering RNA, MAPK activity, synaptic protein levels, and glutamate release decreased. These observations suggest that the IAP plays important roles in dendritic outgrowth and synaptic transmission in developing cortical neurons through the activation of MAPK.

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Numakawa T, Ishimoto T, Suzuki S, Numakawa Y, Adachi N, Matsumoto T et al. Neuronal roles of the integrin-associated protein (IAP/ CD47) in developing cortical neurons. Journal of Biological Chemistry. 2004 Oct 8;279(41):43245-43253. https://doi.org/10.1074/jbc.M406733200