Neurotrophic action of gliostatin on cocultured neurons with glial cells

Takatoshi Ueki, Keiko Nakanishi, Kiyofumi Asai, Yuka Okouchi, Ichiro Isobe, Yaman Z. Eksioglu, Taiji Kato, Kunio Kohno

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24 Citations (Scopus)


Gliostatin is a polypeptide factor (apparent Mr = 100 k with a homodimeric structure comprising two 50 kDa subunits) acting on cortical neurons (neurotrophic action) as well as astrocytic cells (growth inhibition). Under the coculture system of cerebral cortical neurons and astrocytes from fetal rats (E15 or E16), the neurotrophic action of gliostatin was examined immunocytochemically. Immunostaining by an anti-neurofilament (NF) monoclonal antibody visualized a marked neurite-outgrowth and interconnecting bundles of neuritic processes induced by gliostatin in the coculture system. Neurons stimulated by gliostatin formed dense aggregates in clumps, while neurons in control coculture spread out. Gliostatin has also shown survival-promoting effects on neurons. Furthermore, it was shown that gliostatin induced the differentiation of protoplasmic astrocytes to fibrous astrocytes. These results further support our previous contention that gliostatin plays physiological roles on neuronal and glial development.

Original languageEnglish
Pages (from-to)299-302
Number of pages4
JournalBrain Research
Issue number1-2
Publication statusPublished - 17-09-1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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