TY - JOUR
T1 - Neurotrophic action of gliostatin on cocultured neurons with glial cells
AU - Ueki, Takatoshi
AU - Nakanishi, Keiko
AU - Asai, Kiyofumi
AU - Okouchi, Yuka
AU - Isobe, Ichiro
AU - Eksioglu, Yaman Z.
AU - Kato, Taiji
AU - Kohno, Kunio
PY - 1993/9/17
Y1 - 1993/9/17
N2 - Gliostatin is a polypeptide factor (apparent Mr = 100 k with a homodimeric structure comprising two 50 kDa subunits) acting on cortical neurons (neurotrophic action) as well as astrocytic cells (growth inhibition). Under the coculture system of cerebral cortical neurons and astrocytes from fetal rats (E15 or E16), the neurotrophic action of gliostatin was examined immunocytochemically. Immunostaining by an anti-neurofilament (NF) monoclonal antibody visualized a marked neurite-outgrowth and interconnecting bundles of neuritic processes induced by gliostatin in the coculture system. Neurons stimulated by gliostatin formed dense aggregates in clumps, while neurons in control coculture spread out. Gliostatin has also shown survival-promoting effects on neurons. Furthermore, it was shown that gliostatin induced the differentiation of protoplasmic astrocytes to fibrous astrocytes. These results further support our previous contention that gliostatin plays physiological roles on neuronal and glial development.
AB - Gliostatin is a polypeptide factor (apparent Mr = 100 k with a homodimeric structure comprising two 50 kDa subunits) acting on cortical neurons (neurotrophic action) as well as astrocytic cells (growth inhibition). Under the coculture system of cerebral cortical neurons and astrocytes from fetal rats (E15 or E16), the neurotrophic action of gliostatin was examined immunocytochemically. Immunostaining by an anti-neurofilament (NF) monoclonal antibody visualized a marked neurite-outgrowth and interconnecting bundles of neuritic processes induced by gliostatin in the coculture system. Neurons stimulated by gliostatin formed dense aggregates in clumps, while neurons in control coculture spread out. Gliostatin has also shown survival-promoting effects on neurons. Furthermore, it was shown that gliostatin induced the differentiation of protoplasmic astrocytes to fibrous astrocytes. These results further support our previous contention that gliostatin plays physiological roles on neuronal and glial development.
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U2 - 10.1016/0006-8993(93)90833-9
DO - 10.1016/0006-8993(93)90833-9
M3 - Article
C2 - 8242371
AN - SCOPUS:0027161063
VL - 622
SP - 299
EP - 302
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1-2
ER -