TY - JOUR
T1 - Neutrophil accumulation promotes intimal hyperplasia after photochemically induced arterial injury in mice
AU - Shimazawa, Masamitsu
AU - Watanabe, Shinji
AU - Kondo, Kazunao
AU - Hara, Hideaki
AU - Nakashima, Mitsuyoshi
AU - Umemura, Kazuo
PY - 2005/9/27
Y1 - 2005/9/27
N2 - The role of leukocytes in the pathogenesis of coronary arterial disease has become a focus for clinical research. The aim of this study was to determine whether neutrophil accumulation would participate in the development of intimal hyperplasia after endothelial injury in mice, and whether d-myo-inositol hexakisphosphate (phytic acid) which inhibits the binding of L- and P-selectin to sialyl LewisX could inhibit the development of intimal hyperplasia. Endothelial injury was inflicted in one femoral artery via the photochemical reaction between systemically injected rose bengal and transillumination with green light (wavelength: 540 nm). Scanning electron microscopic observation at 3 days after the injury showed an increase in the number of leukocytes adhering to the injury site. Histological observation at 21 days showed that in the neutropenia group administered anti-neutrophil antibody and in the phytic acid-treated group the progression of intimal hyperplasia was significantly attenuated by comparison with the corresponding control groups. These results suggest that neutrophil accumulation contributes to the initiation and/or development of intimal hyperplasia and L- and/or P-selectin may participate in their mechanisms.
AB - The role of leukocytes in the pathogenesis of coronary arterial disease has become a focus for clinical research. The aim of this study was to determine whether neutrophil accumulation would participate in the development of intimal hyperplasia after endothelial injury in mice, and whether d-myo-inositol hexakisphosphate (phytic acid) which inhibits the binding of L- and P-selectin to sialyl LewisX could inhibit the development of intimal hyperplasia. Endothelial injury was inflicted in one femoral artery via the photochemical reaction between systemically injected rose bengal and transillumination with green light (wavelength: 540 nm). Scanning electron microscopic observation at 3 days after the injury showed an increase in the number of leukocytes adhering to the injury site. Histological observation at 21 days showed that in the neutropenia group administered anti-neutrophil antibody and in the phytic acid-treated group the progression of intimal hyperplasia was significantly attenuated by comparison with the corresponding control groups. These results suggest that neutrophil accumulation contributes to the initiation and/or development of intimal hyperplasia and L- and/or P-selectin may participate in their mechanisms.
UR - http://www.scopus.com/inward/record.url?scp=25444463899&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=25444463899&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2005.07.031
DO - 10.1016/j.ejphar.2005.07.031
M3 - Article
C2 - 16140293
AN - SCOPUS:25444463899
SN - 0014-2999
VL - 520
SP - 156
EP - 163
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1-3
ER -