We assessed the effect of varying concentrations of N-formyl-methionyl-leucyl-phenylalanine (fMLP) on neutrophil chemotaxis, phagocytosis, and reactive oxygen species generation. Boyden chamber chemotaxis was first elicited at an fMLP concentration of 10–11 M, reached a peak at 10–10M, and declined at higher concentrations. Phagocytosis was first activated at 10–10M, reached its highest level at 10–9 M, and declined at higher concentrations. O–2, H2O2 and OH• generation were elicited to a significant degree only at a fMLP concentration of 10–8M, or higher, reaching a peak at 10–6 M. Thus, a distinct hierarchy was observed in the order of activation of these three neutrophil functions to varying concentrations of a soluble agonist. A teleologic model of neutrophil function that accounts for these observations is proposed.
All Science Journal Classification (ASJC) codes
- Developmental Neuroscience
- Cellular and Molecular Neuroscience