Neutrophil extracellular traps are induced in a psoriasis model of interleukin-36 receptor antagonist-deficient mice

Soichiro Watanabe, Yohei Iwata, Hidehiko Fukushima, Kenta Saito, Yoshihito Tanaka, Yurie Hasegawa, Masashi Akiyama, Kazumitsu Sugiura

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Loss-of-function mutations in the interleukin (IL)-36 gene IL36RN are associated with psoriasis. The importance of neutrophil extracellular traps (NETs), web-like structures composed of neutrophil DNA, in the pathogenesis of psoriasis has been unclear. Here, we aimed to clarify the role of NET signaling in the deficiency of IL36 receptor antagonist (DITRA). We evaluated the severity of psoriasis-like lesions induced by imiquimod cream treatment in Il36rn−/− mice. The mRNA levels of psoriasis-related cytokines were measured via real-time reverse transcription polymerase chain reaction, and the effects of Cl-amidine, a peptidyl arginine deiminase 4 (PAD4) inhibitor, on psoriasis-like lesions were evaluated. PAD4 is a histone-modifying enzyme that is involved in NET formation. Psoriasis area and severity index scores, epidermal thickness, and infiltrated neutrophil counts were significantly increased in Il36rn−/− mice; NET formation was confirmed pathologically. Several cytokines and chemokines were upregulated in the skin lesions of Il36rn−/− mice and Cl-amidine treatment improved these psoriasis-like lesions. These results suggest that NET formation plays an important role in the pathology of psoriasis-like lesions in these mice and might represent a promising therapeutic target for DITRA.

Original languageEnglish
Article number20149
JournalScientific reports
Volume10
Issue number1
DOIs
Publication statusPublished - 12-2020

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'Neutrophil extracellular traps are induced in a psoriasis model of interleukin-36 receptor antagonist-deficient mice'. Together they form a unique fingerprint.

Cite this