New genotypes of TT virus (TTV) and a genotyping assay based on restriction fragment length polymorphism

Yasuhito Tanaka; Masashi Mizokami; Etsuro Orito; Tomoyoshi Ohno; Tatsunori Nakano; Takanobu Kato; Hideaki Kato; Motokazu Mukaide; Young Min Park; Boo Sung Kim; Ryuzo Ueda

doi:10.1016/S0014-5793(98)01231-9

New genotypes of TT virus (TTV) and a genotyping assay based on restriction fragment length polymorphism

Yasuhito Tanaka, Masashi Mizokami, Etsuro Orito, Tomoyoshi Ohno, Tatsunori Nakano, Takanobu Kato, Hideaki Kato, Motokazu Mukaide, Young Min Park, Boo Sung Kim, Ryuzo Ueda

Research output: Contribution to journal › Article › peer-review

117 Citations (Scopus)

Abstract

A phylogenetic analysis, using the open reading frame 1 sequence of 93 TT viruses (TTV) obtained from various geographical areas, indicated that the virus could be classified into six different genotypes including three hitherto unreported genotypes. The high reliability of the six clusters was confirmed by bootstrap analysis. On the basis of these sequence data, a new simple genotyping assay based on a restriction fragment length polymorphism of TTV was developed. Using the enzymes NdeI and PstI, followed by cleavage with NlaIII or MseI, it was possible to distinguish between the six TTV genotypes. This system will provide the framework for future detailed epidemiological and clinical investigations. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	201-206
Number of pages	6
Journal	FEBS Letters
Volume	437
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)01231-9
Publication status	Published - 23-10-1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(98)01231-9

Cite this

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title = "New genotypes of TT virus (TTV) and a genotyping assay based on restriction fragment length polymorphism",

abstract = "A phylogenetic analysis, using the open reading frame 1 sequence of 93 TT viruses (TTV) obtained from various geographical areas, indicated that the virus could be classified into six different genotypes including three hitherto unreported genotypes. The high reliability of the six clusters was confirmed by bootstrap analysis. On the basis of these sequence data, a new simple genotyping assay based on a restriction fragment length polymorphism of TTV was developed. Using the enzymes NdeI and PstI, followed by cleavage with NlaIII or MseI, it was possible to distinguish between the six TTV genotypes. This system will provide the framework for future detailed epidemiological and clinical investigations. Copyright (C) 1998 Federation of European Biochemical Societies.",

author = "Yasuhito Tanaka and Masashi Mizokami and Etsuro Orito and Tomoyoshi Ohno and Tatsunori Nakano and Takanobu Kato and Hideaki Kato and Motokazu Mukaide and Park, {Young Min} and Kim, {Boo Sung} and Ryuzo Ueda",

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T1 - New genotypes of TT virus (TTV) and a genotyping assay based on restriction fragment length polymorphism

AU - Tanaka, Yasuhito

AU - Mizokami, Masashi

AU - Orito, Etsuro

AU - Ohno, Tomoyoshi

AU - Nakano, Tatsunori

AU - Kato, Takanobu

AU - Kato, Hideaki

AU - Mukaide, Motokazu

AU - Park, Young Min

AU - Kim, Boo Sung

AU - Ueda, Ryuzo

PY - 1998/10/23

Y1 - 1998/10/23

N2 - A phylogenetic analysis, using the open reading frame 1 sequence of 93 TT viruses (TTV) obtained from various geographical areas, indicated that the virus could be classified into six different genotypes including three hitherto unreported genotypes. The high reliability of the six clusters was confirmed by bootstrap analysis. On the basis of these sequence data, a new simple genotyping assay based on a restriction fragment length polymorphism of TTV was developed. Using the enzymes NdeI and PstI, followed by cleavage with NlaIII or MseI, it was possible to distinguish between the six TTV genotypes. This system will provide the framework for future detailed epidemiological and clinical investigations. Copyright (C) 1998 Federation of European Biochemical Societies.

AB - A phylogenetic analysis, using the open reading frame 1 sequence of 93 TT viruses (TTV) obtained from various geographical areas, indicated that the virus could be classified into six different genotypes including three hitherto unreported genotypes. The high reliability of the six clusters was confirmed by bootstrap analysis. On the basis of these sequence data, a new simple genotyping assay based on a restriction fragment length polymorphism of TTV was developed. Using the enzymes NdeI and PstI, followed by cleavage with NlaIII or MseI, it was possible to distinguish between the six TTV genotypes. This system will provide the framework for future detailed epidemiological and clinical investigations. Copyright (C) 1998 Federation of European Biochemical Societies.

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New genotypes of TT virus (TTV) and a genotyping assay based on restriction fragment length polymorphism

Abstract

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