New genotypes of TT virus (TTV) and a genotyping assay based on restriction fragment length polymorphism

Yasuhito Tanaka, Masashi Mizokami, Etsuro Orito, Tomoyoshi Ohno, Tatsunori Nakano, Takanobu Kato, Hideaki Kato, Motokazu Mukaide, Young Min Park, Boo Sung Kim, Ryuzo Ueda

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Abstract

A phylogenetic analysis, using the open reading frame 1 sequence of 93 TT viruses (TTV) obtained from various geographical areas, indicated that the virus could be classified into six different genotypes including three hitherto unreported genotypes. The high reliability of the six clusters was confirmed by bootstrap analysis. On the basis of these sequence data, a new simple genotyping assay based on a restriction fragment length polymorphism of TTV was developed. Using the enzymes NdeI and PstI, followed by cleavage with NlaIII or MseI, it was possible to distinguish between the six TTV genotypes. This system will provide the framework for future detailed epidemiological and clinical investigations. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalFEBS Letters
Volume437
Issue number3
DOIs
Publication statusPublished - 23-10-1998

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Tanaka, Y., Mizokami, M., Orito, E., Ohno, T., Nakano, T., Kato, T., Kato, H., Mukaide, M., Park, Y. M., Kim, B. S., & Ueda, R. (1998). New genotypes of TT virus (TTV) and a genotyping assay based on restriction fragment length polymorphism. FEBS Letters, 437(3), 201-206. https://doi.org/10.1016/S0014-5793(98)01231-9