New Grocott stain without using chromic acid

Kazuya Shiogama, Kayo Kitazawa, Yasuyoshi Mizutani, Takanori Onouchi, Ken Ichi Inada, Yutaka Tsutsumi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We established a new “ecological” Grocott stain for demonstrating fungi, based upon a 4R principle of refusal, reduction, reuse, and recycle of waste management. Conventional Grocott stain employs environmentally harsh 5% chromic acid for oxidization. Initially, we succeeded in reducing the concentration of chromic acid from 5% to 1% by incubating the solution at 60°C and using five-fold diluted chromic acid solution at which point it was reusable. Eventually, we reached the refusal level where 1% periodic acid oxidization was efficient enough, when combined with preheating of sections in the electric jar, microwave oven, or pressure pan. For convenience sake, we recommend pressure pan heating in tap water for 10 min. Stainability of fungi in candidiasis and aspergillosis was comparable with conventional Grocott stain, while Mucor hyphae showed enhanced staining. The modified sequence was further applicable to detecting a variety of mycotic pathogens in paraffin sections. Our environmentally-friendly Grocott stain also has the advantage of avoiding risk of human exposure to hexavalent chromium solution in the histopathology laboratory. The simple stain sequence is can be easily applied worldwide.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalActa Histochemica et Cytochemica
Volume48
Issue number1
DOIs
Publication statusPublished - 15-01-2015

Fingerprint

Coloring Agents
Fungi
Waste Management
Mucor
Microwave ovens
Pressure
Periodic Acid
Aspergillosis
Hyphae
Candidiasis
Preheating
Pathogens
Waste management
Microwaves
Paraffin
Heating
chromic acid
Staining and Labeling
Water

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

Cite this

Shiogama, Kazuya ; Kitazawa, Kayo ; Mizutani, Yasuyoshi ; Onouchi, Takanori ; Inada, Ken Ichi ; Tsutsumi, Yutaka. / New Grocott stain without using chromic acid. In: Acta Histochemica et Cytochemica. 2015 ; Vol. 48, No. 1. pp. 9-14.
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New Grocott stain without using chromic acid. / Shiogama, Kazuya; Kitazawa, Kayo; Mizutani, Yasuyoshi; Onouchi, Takanori; Inada, Ken Ichi; Tsutsumi, Yutaka.

In: Acta Histochemica et Cytochemica, Vol. 48, No. 1, 15.01.2015, p. 9-14.

Research output: Contribution to journalArticle

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