NGFI-B, c-fos, and c-jun mRNA expression in mouse brain after acute carbon monoxide intoxication

Ya Ping Tang, Yoshiharu Murata, Takashi Nagaya, Yukihiro Noda, Hisao Seo, Toshitaka Nabeshima

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The expression of immediate early genes (IEG) has been documented in the brain after various kinds of insults such as ischemia and hypoxia. To determine whether acute carbon monoxide intoxication (ACOI) might trigger IEG expression, adult ddY mice were subjected to carbon monoxide exposure at a rate of 30 mL/min for 35 seconds. The levels of NGFI-B, c-fos, and c-jun mRNA were determined by Northern blot analysis. A time-course study in the cerebral cortex indicated that the induction of NGFI-B, c-fos, and c-jun mRNA started as early as 15 minutes, reached a peak at 30 minutes, and returned to the basal level at 1 hour after the ACOI. In addition, the temporal feature of the induction of these IEG mRNA in the hippocampus was very similar to that in the cerebral cortex. Examination of brain regions at 30 minutes after the ACOI revealed a significant induction of NGFI-B mRNA in the cerebellum, thalamus-hypothalamus, brainstem, as well as in the cortex and hippocampus, but not in the striatum or olfactory bulb. Furthermore, the neuroanatomical distribution of c-fos mRNA at 30 minutes after the ACOI was very similar to that of the NGFI-B mRNA. The widespread distribution of these IEG in the brain, especially in the cerebellum and brainstem, indicates that the major cause for the triggering of IEG expression in the brain by the ACOI might be a diffuse hypoxia. These findings show for the first time the temporal and spatial expression of IEG in the brain after ACOI.

Original languageEnglish
Pages (from-to)771-780
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume17
Issue number7
DOIs
Publication statusPublished - 01-01-1997
Externally publishedYes

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Carbon Monoxide
Immediate-Early Genes
Messenger RNA
Brain
Cerebral Cortex
Cerebellum
Brain Stem
Hippocampus
Gene Expression
Olfactory Bulb
Thalamus
Northern Blotting
Hypothalamus
Ischemia

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Tang, Ya Ping ; Murata, Yoshiharu ; Nagaya, Takashi ; Noda, Yukihiro ; Seo, Hisao ; Nabeshima, Toshitaka. / NGFI-B, c-fos, and c-jun mRNA expression in mouse brain after acute carbon monoxide intoxication. In: Journal of Cerebral Blood Flow and Metabolism. 1997 ; Vol. 17, No. 7. pp. 771-780.
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abstract = "The expression of immediate early genes (IEG) has been documented in the brain after various kinds of insults such as ischemia and hypoxia. To determine whether acute carbon monoxide intoxication (ACOI) might trigger IEG expression, adult ddY mice were subjected to carbon monoxide exposure at a rate of 30 mL/min for 35 seconds. The levels of NGFI-B, c-fos, and c-jun mRNA were determined by Northern blot analysis. A time-course study in the cerebral cortex indicated that the induction of NGFI-B, c-fos, and c-jun mRNA started as early as 15 minutes, reached a peak at 30 minutes, and returned to the basal level at 1 hour after the ACOI. In addition, the temporal feature of the induction of these IEG mRNA in the hippocampus was very similar to that in the cerebral cortex. Examination of brain regions at 30 minutes after the ACOI revealed a significant induction of NGFI-B mRNA in the cerebellum, thalamus-hypothalamus, brainstem, as well as in the cortex and hippocampus, but not in the striatum or olfactory bulb. Furthermore, the neuroanatomical distribution of c-fos mRNA at 30 minutes after the ACOI was very similar to that of the NGFI-B mRNA. The widespread distribution of these IEG in the brain, especially in the cerebellum and brainstem, indicates that the major cause for the triggering of IEG expression in the brain by the ACOI might be a diffuse hypoxia. These findings show for the first time the temporal and spatial expression of IEG in the brain after ACOI.",
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NGFI-B, c-fos, and c-jun mRNA expression in mouse brain after acute carbon monoxide intoxication. / Tang, Ya Ping; Murata, Yoshiharu; Nagaya, Takashi; Noda, Yukihiro; Seo, Hisao; Nabeshima, Toshitaka.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 17, No. 7, 01.01.1997, p. 771-780.

Research output: Contribution to journalArticle

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T1 - NGFI-B, c-fos, and c-jun mRNA expression in mouse brain after acute carbon monoxide intoxication

AU - Tang, Ya Ping

AU - Murata, Yoshiharu

AU - Nagaya, Takashi

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AU - Seo, Hisao

AU - Nabeshima, Toshitaka

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