Nicotine regulates mRNA level of tyrosine hydroxylase gene but not that of nicotinic acetylcholine receptor genes in PC12 cells

Hiroshi Ishiguro, Naohiro Ichino, Koji Yamada, Toshiharu Nagatsu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To understand the molecular mechanism of nicotine addiction, we examined the mRNA level of the tyrosine hydroxylase (TH) gene and that of the nicotinic acetylcholine receptor (nAChR) genes by long term nicotine treatment. The transcript levels of the four subunit genes of the nAChR (α3, α5, α7, and β4) were down-regulated by the treatment with forskolin, whereas the mRNA levels of the TH gene was increased in PC12 cells. By long- term nicotine treatment, the mRNA level of the nAChR genes did not change, but transcript levels of α3, α5, α7, and β4 nAChR genes were still negatively regulated by forskolin. However, the mRNA level of TH gene did not change by forskolin under long-term nicotine treatment. The TH gene may be regulated by a nicotine-related signaling pathway, whereas α3, α5, α7, and β4 nAChR genes may be further regulated by a protein kinase A (PKA) pathway under long-term nicotine treatment.

Original languageEnglish
Pages (from-to)37-40
Number of pages4
JournalNeuroscience Letters
Volume228
Issue number1
DOIs
Publication statusPublished - 30-05-1997

Fingerprint

PC12 Cells
Nicotinic Receptors
Tyrosine 3-Monooxygenase
Nicotine
Messenger RNA
Genes
Colforsin
Cyclic AMP-Dependent Protein Kinases

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{ac2edff31c454df38ba1038eaa908f3a,
title = "Nicotine regulates mRNA level of tyrosine hydroxylase gene but not that of nicotinic acetylcholine receptor genes in PC12 cells",
abstract = "To understand the molecular mechanism of nicotine addiction, we examined the mRNA level of the tyrosine hydroxylase (TH) gene and that of the nicotinic acetylcholine receptor (nAChR) genes by long term nicotine treatment. The transcript levels of the four subunit genes of the nAChR (α3, α5, α7, and β4) were down-regulated by the treatment with forskolin, whereas the mRNA levels of the TH gene was increased in PC12 cells. By long- term nicotine treatment, the mRNA level of the nAChR genes did not change, but transcript levels of α3, α5, α7, and β4 nAChR genes were still negatively regulated by forskolin. However, the mRNA level of TH gene did not change by forskolin under long-term nicotine treatment. The TH gene may be regulated by a nicotine-related signaling pathway, whereas α3, α5, α7, and β4 nAChR genes may be further regulated by a protein kinase A (PKA) pathway under long-term nicotine treatment.",
author = "Hiroshi Ishiguro and Naohiro Ichino and Koji Yamada and Toshiharu Nagatsu",
year = "1997",
month = "5",
day = "30",
doi = "10.1016/S0304-3940(97)00360-1",
language = "English",
volume = "228",
pages = "37--40",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Nicotine regulates mRNA level of tyrosine hydroxylase gene but not that of nicotinic acetylcholine receptor genes in PC12 cells. / Ishiguro, Hiroshi; Ichino, Naohiro; Yamada, Koji; Nagatsu, Toshiharu.

In: Neuroscience Letters, Vol. 228, No. 1, 30.05.1997, p. 37-40.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nicotine regulates mRNA level of tyrosine hydroxylase gene but not that of nicotinic acetylcholine receptor genes in PC12 cells

AU - Ishiguro, Hiroshi

AU - Ichino, Naohiro

AU - Yamada, Koji

AU - Nagatsu, Toshiharu

PY - 1997/5/30

Y1 - 1997/5/30

N2 - To understand the molecular mechanism of nicotine addiction, we examined the mRNA level of the tyrosine hydroxylase (TH) gene and that of the nicotinic acetylcholine receptor (nAChR) genes by long term nicotine treatment. The transcript levels of the four subunit genes of the nAChR (α3, α5, α7, and β4) were down-regulated by the treatment with forskolin, whereas the mRNA levels of the TH gene was increased in PC12 cells. By long- term nicotine treatment, the mRNA level of the nAChR genes did not change, but transcript levels of α3, α5, α7, and β4 nAChR genes were still negatively regulated by forskolin. However, the mRNA level of TH gene did not change by forskolin under long-term nicotine treatment. The TH gene may be regulated by a nicotine-related signaling pathway, whereas α3, α5, α7, and β4 nAChR genes may be further regulated by a protein kinase A (PKA) pathway under long-term nicotine treatment.

AB - To understand the molecular mechanism of nicotine addiction, we examined the mRNA level of the tyrosine hydroxylase (TH) gene and that of the nicotinic acetylcholine receptor (nAChR) genes by long term nicotine treatment. The transcript levels of the four subunit genes of the nAChR (α3, α5, α7, and β4) were down-regulated by the treatment with forskolin, whereas the mRNA levels of the TH gene was increased in PC12 cells. By long- term nicotine treatment, the mRNA level of the nAChR genes did not change, but transcript levels of α3, α5, α7, and β4 nAChR genes were still negatively regulated by forskolin. However, the mRNA level of TH gene did not change by forskolin under long-term nicotine treatment. The TH gene may be regulated by a nicotine-related signaling pathway, whereas α3, α5, α7, and β4 nAChR genes may be further regulated by a protein kinase A (PKA) pathway under long-term nicotine treatment.

UR - http://www.scopus.com/inward/record.url?scp=0030753886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030753886&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(97)00360-1

DO - 10.1016/S0304-3940(97)00360-1

M3 - Article

C2 - 9197282

AN - SCOPUS:0030753886

VL - 228

SP - 37

EP - 40

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -