Nicotine withdrawal up-regulates c-Fos transcription in pheochromocytoma cells

Naohiro Ichino; Hiroshi Ishiguro; Kouji Yamada; Kazuhiro Nishii; Hirohide Sawada; Toshiharu Nagatsu

doi:10.1016/S0168-0102(99)00069-3

Nicotine withdrawal up-regulates c-Fos transcription in pheochromocytoma cells

Naohiro Ichino
, Hiroshi Ishiguro
, Kouji Yamada
, Kazuhiro Nishii
, Hirohide Sawada
, Toshiharu Nagatsu

Clinical Physiology

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The influence of nicotine on the expression of Fos family proteins, which specifically formed complexes with the AP-1 sequence, was assessed. mRNA for c-Fos, c-jun and jun-B were up-regulated at 0.5 h after nicotine treatment, elevated c-Fos also being apparent after withdrawal. Although nicotine failed to up-regulate the mRNA level of the fra-1 gene, the Fra-1 protein was highly expressed after both treatment and withdrawal. These results indicate that nicotine treatment may affect the transcriptional activity of many genes through c-Fos and c-Jun protein expression in neural cells, and that Fra-1 protein may make a contribution. These changes in immediately early genes (IEGs) may be associated with nicotine withdrawal symptoms.

Original language	English
Pages (from-to)	63-69
Number of pages	7
Journal	Neuroscience Research
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/S0168-0102(99)00069-3
Publication status	Published - 10-1999

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1016/S0168-0102(99)00069-3

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@article{f0f4099b04b8499c9ceaa5dfe25116bf,

title = "Nicotine withdrawal up-regulates c-Fos transcription in pheochromocytoma cells",

abstract = "The influence of nicotine on the expression of Fos family proteins, which specifically formed complexes with the AP-1 sequence, was assessed. mRNA for c-Fos, c-jun and jun-B were up-regulated at 0.5 h after nicotine treatment, elevated c-Fos also being apparent after withdrawal. Although nicotine failed to up-regulate the mRNA level of the fra-1 gene, the Fra-1 protein was highly expressed after both treatment and withdrawal. These results indicate that nicotine treatment may affect the transcriptional activity of many genes through c-Fos and c-Jun protein expression in neural cells, and that Fra-1 protein may make a contribution. These changes in immediately early genes (IEGs) may be associated with nicotine withdrawal symptoms.",

keywords = "C-Fos, Fos-related antigen-1 (Fra-1), Immediately early genes, Nicotine, Nicotinic acetylcholine receptors, PC12 cells, Withdrawal",

author = "Naohiro Ichino and Hiroshi Ishiguro and Kouji Yamada and Kazuhiro Nishii and Hirohide Sawada and Toshiharu Nagatsu",

note = "Funding Information: This work was supported by a grant from the Smoking Research Foundation and a grant-in-aid from Fujita Health University.",

year = "1999",

month = oct,

doi = "10.1016/S0168-0102(99)00069-3",

language = "English",

volume = "35",

pages = "63--69",

journal = "Neuroscience Research",

issn = "0168-0102",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Nicotine withdrawal up-regulates c-Fos transcription in pheochromocytoma cells

AU - Ichino, Naohiro

AU - Ishiguro, Hiroshi

AU - Yamada, Kouji

AU - Nishii, Kazuhiro

AU - Sawada, Hirohide

AU - Nagatsu, Toshiharu

N1 - Funding Information: This work was supported by a grant from the Smoking Research Foundation and a grant-in-aid from Fujita Health University.

PY - 1999/10

Y1 - 1999/10

N2 - The influence of nicotine on the expression of Fos family proteins, which specifically formed complexes with the AP-1 sequence, was assessed. mRNA for c-Fos, c-jun and jun-B were up-regulated at 0.5 h after nicotine treatment, elevated c-Fos also being apparent after withdrawal. Although nicotine failed to up-regulate the mRNA level of the fra-1 gene, the Fra-1 protein was highly expressed after both treatment and withdrawal. These results indicate that nicotine treatment may affect the transcriptional activity of many genes through c-Fos and c-Jun protein expression in neural cells, and that Fra-1 protein may make a contribution. These changes in immediately early genes (IEGs) may be associated with nicotine withdrawal symptoms.

AB - The influence of nicotine on the expression of Fos family proteins, which specifically formed complexes with the AP-1 sequence, was assessed. mRNA for c-Fos, c-jun and jun-B were up-regulated at 0.5 h after nicotine treatment, elevated c-Fos also being apparent after withdrawal. Although nicotine failed to up-regulate the mRNA level of the fra-1 gene, the Fra-1 protein was highly expressed after both treatment and withdrawal. These results indicate that nicotine treatment may affect the transcriptional activity of many genes through c-Fos and c-Jun protein expression in neural cells, and that Fra-1 protein may make a contribution. These changes in immediately early genes (IEGs) may be associated with nicotine withdrawal symptoms.

KW - C-Fos

KW - Fos-related antigen-1 (Fra-1)

KW - Immediately early genes

KW - Nicotine

KW - Nicotinic acetylcholine receptors

KW - PC12 cells

KW - Withdrawal

UR - https://www.scopus.com/pages/publications/0032828413

UR - https://www.scopus.com/pages/publications/0032828413#tab=citedBy

U2 - 10.1016/S0168-0102(99)00069-3

DO - 10.1016/S0168-0102(99)00069-3

M3 - Article

C2 - 10555165

AN - SCOPUS:0032828413

SN - 0168-0102

VL - 35

SP - 63

EP - 69

JO - Neuroscience Research

JF - Neuroscience Research

IS - 1

ER -

Nicotine withdrawal up-regulates c-Fos transcription in pheochromocytoma cells

Abstract

All Science Journal Classification (ASJC) codes

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