Nigral GABAergic inhibition upon cholinergic neurons in the rat pedunculopontine tegmental nucleus

Kazuya Saitoh, Satoko Takai, Wen Jie Song, Tadashi Isa, Kaoru Takakusaki

Research output: Contribution to journalArticle

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Abstract

We investigated, in a midbrain parasagittal slice preparation of Wistar rats (postnatal day 9-17), the synaptic inhibition of neurons in the pedunculopontine tegmental nucleus (PPN), which was mediated by gamma (γ)-amino-butyric acid (GABA). Whole-cell patch-clamp recording was used, in combination with a single-cell reverse transcription-polymerase chain reaction amplification technique, to record synaptic potentials and to identify the phenotype of the recorded PPN neuron. In the presence of the ionotropic glutamate receptor antagonists, 6-cyano-2, 3-dihydroxy-7-nitro-quinoxaline-2, 3, dione, and DL-2-amino-5-phosphonovaleric acid, single electrical stimuli were applied to the substantia nigra pars reticulata (SNr), one of the basal ganglia output nuclei. Stimulation of the SNr evoked inhibitory postsynaptic potentials (IPSPs) in 73 of the 104 neurons in the PPN. The IPSPs were abolished with a GABAA receptor antagonist, bicuculline. Inhibitory postsynaptic currents of the neurons were reversed in polarity at approximately -93.5 mV, which was close to the value of the equilibrium potential for chloride ions of -88.4 mV. Single-cell reverse transcription-polymerase chain reactions revealed that approximately 30% (9/32) of the PPN neurons that received inhibition from the SNr expressed detectable levels of choline acetyltransferase mRNA. These findings show that output from the SNr regulates the activity of cholinergic PPN neurons through GABAA receptors.

Original languageEnglish
Pages (from-to)879-886
Number of pages8
JournalEuropean Journal of Neuroscience
Volume18
Issue number4
DOIs
Publication statusPublished - 01-08-2003

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Pedunculopontine Tegmental Nucleus
Cholinergic Neurons
Substantia Nigra
Neurons
Inhibitory Postsynaptic Potentials
Reverse Transcription
2-Amino-5-phosphonovalerate
Ionotropic Glutamate Receptors
GABA-A Receptor Antagonists
Quinoxalines
Excitatory Amino Acid Antagonists
Polymerase Chain Reaction
Synaptic Potentials
Choline O-Acetyltransferase
Butyric Acid
Bicuculline
GABA-A Receptors
Mesencephalon
Basal Ganglia
Cholinergic Agents

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Saitoh, Kazuya ; Takai, Satoko ; Song, Wen Jie ; Isa, Tadashi ; Takakusaki, Kaoru. / Nigral GABAergic inhibition upon cholinergic neurons in the rat pedunculopontine tegmental nucleus. In: European Journal of Neuroscience. 2003 ; Vol. 18, No. 4. pp. 879-886.
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Nigral GABAergic inhibition upon cholinergic neurons in the rat pedunculopontine tegmental nucleus. / Saitoh, Kazuya; Takai, Satoko; Song, Wen Jie; Isa, Tadashi; Takakusaki, Kaoru.

In: European Journal of Neuroscience, Vol. 18, No. 4, 01.08.2003, p. 879-886.

Research output: Contribution to journalArticle

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T1 - Nigral GABAergic inhibition upon cholinergic neurons in the rat pedunculopontine tegmental nucleus

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