No associations found between the genes situated at 6p22.1, HIST1H2BJ, PRSS16, and PGBD1 in Japanese patients diagnosed with schizophrenia

Maiko Kitazawa, Tohru Ohnuma, Yuto Takebayashi, Nobuto Shibata, Hajime Baba, Kazutaka Ohi, Yuka Yasuda, Yukako Nakamura, Branko Aleksic, Akira Yoshimi, Tomo Okochi, Masashi Ikeda, Hiroshi Naitoh, Ryota Hashimoto, Nakao Iwata, Norio Ozaki, Masatoshi Takeda, Heii Arai

Research output: Contribution to journalArticle

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Abstract

Recent GWAS demonstrated an association between candidate genes located at region 6p22.1 and schizophrenia. This region has been reported to house certain candidate SNPs, which may be associated with schizophrenia at HIST1H2BJ, PRSS16, and PGBD1. These genes may presumably be associated with pathophysiology in schizophrenia, namely epigenetics and psychoneuroimmunology. A three-step study was undertaken to focus on these genes with the following aims: (1) whether these genes may be associated in Japanese patients with schizophrenia by performing a 1st stage case-control study (514 cases and 706 controls) using Japanese tagging SNPs; (2) if the genetic regions of interest for the disease from the 1st stage of analyses were found, re-sequencing was performed to search for new mutations; (3) finally, a replication study was undertaken to confirm positive findings from the 1st stage were reconfirmed using a larger number of subjects (2,583 cases and 2,903 controls) during a 2nd stage multicenter replication study in Japan. Genotyping was performed using TaqMan PCR method for the selected nine tagging SNPs. Although three SNPs situated at the 3′ side of PGBD1; rs3800324, rs3800327, and rs2142730, and two-window haplotypes between rs3800327 and rs2142730 showed positive associations with schizophrenia, these associations did not have enough power to sustain significance during the 2nd stage replication study. In addition, re-sequencing for exons 5 and 6 situated at this region did not express any new mutations for schizophrenia. Taken together these results indicate that the genes HIST1H2BJ, PRSS16, and PGBD1 were not associated with Japanese patients with schizophrenia.

Original languageEnglish
Pages (from-to)456-464
Number of pages9
JournalAmerican Journal of Medical Genetics, Part B: Neuropsychiatric Genetics
Volume159 B
Issue number4
DOIs
Publication statusPublished - 01-06-2012

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Schizophrenia
Single Nucleotide Polymorphism
Genes
Psychoneuroimmunology
Mutation
Genome-Wide Association Study
Epigenomics
Haplotypes
Multicenter Studies
Case-Control Studies
Exons
Japan
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

Cite this

Kitazawa, Maiko ; Ohnuma, Tohru ; Takebayashi, Yuto ; Shibata, Nobuto ; Baba, Hajime ; Ohi, Kazutaka ; Yasuda, Yuka ; Nakamura, Yukako ; Aleksic, Branko ; Yoshimi, Akira ; Okochi, Tomo ; Ikeda, Masashi ; Naitoh, Hiroshi ; Hashimoto, Ryota ; Iwata, Nakao ; Ozaki, Norio ; Takeda, Masatoshi ; Arai, Heii. / No associations found between the genes situated at 6p22.1, HIST1H2BJ, PRSS16, and PGBD1 in Japanese patients diagnosed with schizophrenia. In: American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics. 2012 ; Vol. 159 B, No. 4. pp. 456-464.
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title = "No associations found between the genes situated at 6p22.1, HIST1H2BJ, PRSS16, and PGBD1 in Japanese patients diagnosed with schizophrenia",
abstract = "Recent GWAS demonstrated an association between candidate genes located at region 6p22.1 and schizophrenia. This region has been reported to house certain candidate SNPs, which may be associated with schizophrenia at HIST1H2BJ, PRSS16, and PGBD1. These genes may presumably be associated with pathophysiology in schizophrenia, namely epigenetics and psychoneuroimmunology. A three-step study was undertaken to focus on these genes with the following aims: (1) whether these genes may be associated in Japanese patients with schizophrenia by performing a 1st stage case-control study (514 cases and 706 controls) using Japanese tagging SNPs; (2) if the genetic regions of interest for the disease from the 1st stage of analyses were found, re-sequencing was performed to search for new mutations; (3) finally, a replication study was undertaken to confirm positive findings from the 1st stage were reconfirmed using a larger number of subjects (2,583 cases and 2,903 controls) during a 2nd stage multicenter replication study in Japan. Genotyping was performed using TaqMan PCR method for the selected nine tagging SNPs. Although three SNPs situated at the 3′ side of PGBD1; rs3800324, rs3800327, and rs2142730, and two-window haplotypes between rs3800327 and rs2142730 showed positive associations with schizophrenia, these associations did not have enough power to sustain significance during the 2nd stage replication study. In addition, re-sequencing for exons 5 and 6 situated at this region did not express any new mutations for schizophrenia. Taken together these results indicate that the genes HIST1H2BJ, PRSS16, and PGBD1 were not associated with Japanese patients with schizophrenia.",
author = "Maiko Kitazawa and Tohru Ohnuma and Yuto Takebayashi and Nobuto Shibata and Hajime Baba and Kazutaka Ohi and Yuka Yasuda and Yukako Nakamura and Branko Aleksic and Akira Yoshimi and Tomo Okochi and Masashi Ikeda and Hiroshi Naitoh and Ryota Hashimoto and Nakao Iwata and Norio Ozaki and Masatoshi Takeda and Heii Arai",
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Kitazawa, M, Ohnuma, T, Takebayashi, Y, Shibata, N, Baba, H, Ohi, K, Yasuda, Y, Nakamura, Y, Aleksic, B, Yoshimi, A, Okochi, T, Ikeda, M, Naitoh, H, Hashimoto, R, Iwata, N, Ozaki, N, Takeda, M & Arai, H 2012, 'No associations found between the genes situated at 6p22.1, HIST1H2BJ, PRSS16, and PGBD1 in Japanese patients diagnosed with schizophrenia', American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics, vol. 159 B, no. 4, pp. 456-464. https://doi.org/10.1002/ajmg.b.32049

No associations found between the genes situated at 6p22.1, HIST1H2BJ, PRSS16, and PGBD1 in Japanese patients diagnosed with schizophrenia. / Kitazawa, Maiko; Ohnuma, Tohru; Takebayashi, Yuto; Shibata, Nobuto; Baba, Hajime; Ohi, Kazutaka; Yasuda, Yuka; Nakamura, Yukako; Aleksic, Branko; Yoshimi, Akira; Okochi, Tomo; Ikeda, Masashi; Naitoh, Hiroshi; Hashimoto, Ryota; Iwata, Nakao; Ozaki, Norio; Takeda, Masatoshi; Arai, Heii.

In: American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics, Vol. 159 B, No. 4, 01.06.2012, p. 456-464.

Research output: Contribution to journalArticle

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T1 - No associations found between the genes situated at 6p22.1, HIST1H2BJ, PRSS16, and PGBD1 in Japanese patients diagnosed with schizophrenia

AU - Kitazawa, Maiko

AU - Ohnuma, Tohru

AU - Takebayashi, Yuto

AU - Shibata, Nobuto

AU - Baba, Hajime

AU - Ohi, Kazutaka

AU - Yasuda, Yuka

AU - Nakamura, Yukako

AU - Aleksic, Branko

AU - Yoshimi, Akira

AU - Okochi, Tomo

AU - Ikeda, Masashi

AU - Naitoh, Hiroshi

AU - Hashimoto, Ryota

AU - Iwata, Nakao

AU - Ozaki, Norio

AU - Takeda, Masatoshi

AU - Arai, Heii

PY - 2012/6/1

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N2 - Recent GWAS demonstrated an association between candidate genes located at region 6p22.1 and schizophrenia. This region has been reported to house certain candidate SNPs, which may be associated with schizophrenia at HIST1H2BJ, PRSS16, and PGBD1. These genes may presumably be associated with pathophysiology in schizophrenia, namely epigenetics and psychoneuroimmunology. A three-step study was undertaken to focus on these genes with the following aims: (1) whether these genes may be associated in Japanese patients with schizophrenia by performing a 1st stage case-control study (514 cases and 706 controls) using Japanese tagging SNPs; (2) if the genetic regions of interest for the disease from the 1st stage of analyses were found, re-sequencing was performed to search for new mutations; (3) finally, a replication study was undertaken to confirm positive findings from the 1st stage were reconfirmed using a larger number of subjects (2,583 cases and 2,903 controls) during a 2nd stage multicenter replication study in Japan. Genotyping was performed using TaqMan PCR method for the selected nine tagging SNPs. Although three SNPs situated at the 3′ side of PGBD1; rs3800324, rs3800327, and rs2142730, and two-window haplotypes between rs3800327 and rs2142730 showed positive associations with schizophrenia, these associations did not have enough power to sustain significance during the 2nd stage replication study. In addition, re-sequencing for exons 5 and 6 situated at this region did not express any new mutations for schizophrenia. Taken together these results indicate that the genes HIST1H2BJ, PRSS16, and PGBD1 were not associated with Japanese patients with schizophrenia.

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