Noncanonical TATA sequence in the UL44 late promoter of human cytomegalovirus is required for the accumulation of late viral transcripts

Hiroki Isomura, Mark F. Stinski, Ayumi Kudoh, Takayuki Murata, Sanae Nakayama, Yoshitaka Sato, Satoko Iwahori, Tatsuya Tsurumi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

During productive infection, human cytomegalovirus (HCMV) UL44 transcription initiates at three distinct start sites that are differentially regulated. Two of the start sites, the distal and the proximal, are active at early times, whereas the middle start site is active only at late times after infection. The UL44 early viral gene product is essential for viral DNA synthesis. The UL44 gene product from the late viral promoter affects primarily viral gene expression at late times after infection rather than viral DNA synthesis (H. Isomura, M. F. Stinski, A. Kudoh, S. Nakayama, S. Iwahori, Y. Sato, and T. Tsurumi, J. Virol. 81:6197, 2007). The UL44 early viral promoters have a canonical TATA sequence, "TATAA." In contrast, the UL44 late viral promoter has a noncanonical TATA sequence. Using recombinant viruses, we found that the noncanonical TATA sequence is required for the accumulation of late viral transcripts. The GC boxes that surround the middle TATA element did not affect the kinetics or the start site of UL44 late transcription. Replacement of the distal TATA element with a noncanonical TATA sequence did not affect the kinetics of transcription or the transcription start site, but it did induce an alternative transcript at late times after infection. The data indicate that a noncanonical TATA box is used at late times after HCMV infection.

Original languageEnglish
Pages (from-to)1638-1646
Number of pages9
JournalJournal of Virology
Volume82
Issue number4
DOIs
Publication statusPublished - 01-02-2008

Fingerprint

Human herpesvirus 5
Cytomegalovirus
transcription (genetics)
promoter regions
infection
Viral DNA
Viral Proteins
Infection
kinetics
TATA box
synthesis
DNA
TATA Box
Viral Genes
Transcription Initiation Site
Cytomegalovirus Infections
genes
Catalytic Domain
gene expression
viruses

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Isomura, Hiroki ; Stinski, Mark F. ; Kudoh, Ayumi ; Murata, Takayuki ; Nakayama, Sanae ; Sato, Yoshitaka ; Iwahori, Satoko ; Tsurumi, Tatsuya. / Noncanonical TATA sequence in the UL44 late promoter of human cytomegalovirus is required for the accumulation of late viral transcripts. In: Journal of Virology. 2008 ; Vol. 82, No. 4. pp. 1638-1646.
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Noncanonical TATA sequence in the UL44 late promoter of human cytomegalovirus is required for the accumulation of late viral transcripts. / Isomura, Hiroki; Stinski, Mark F.; Kudoh, Ayumi; Murata, Takayuki; Nakayama, Sanae; Sato, Yoshitaka; Iwahori, Satoko; Tsurumi, Tatsuya.

In: Journal of Virology, Vol. 82, No. 4, 01.02.2008, p. 1638-1646.

Research output: Contribution to journalArticle

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