Nongenetic method for purifying stem cell-derived cardiomyocytes

Fumiyuki Hattori; Hao Chen; Hiromi Yamashita; Shugo Tohyama; Yu Suke Satoh; Shinsuke Yuasa; Weizhen Li; Hiroyuki Yamakawa; Tomofumi Tanaka; Takeshi Onitsuka; Kenichiro Shimoji; Yohei Ohno; Toru Egashira; Ruri Kaneda; Mitsushige Murata; Kyoko Hidaka; Takayuki Morisaki; Erika Sasaki; Takeshi Suzuki; Motoaki Sano; Shinji Makino; Shinzo Oikawa; Keiichi Fukuda

doi:10.1038/nmeth.1403

Nongenetic method for purifying stem cell-derived cardiomyocytes

Fumiyuki Hattori, Hao Chen, Hiromi Yamashita, Shugo Tohyama, Yu Suke Satoh, Shinsuke Yuasa, Weizhen Li, Hiroyuki Yamakawa, Tomofumi Tanaka, Takeshi Onitsuka, Kenichiro Shimoji, Yohei Ohno, Toru Egashira, Ruri Kaneda, Mitsushige Murata, Kyoko Hidaka, Takayuki Morisaki, Erika Sasaki, Takeshi Suzuki, Motoaki SanoShinji Makino, Shinzo Oikawa, Keiichi Fukuda

Research output: Contribution to journal › Article › peer-review

346 Citations (Scopus)

Abstract

Several applications of pluripotent stem cell (PSC)-derlved cardlomyocytes require elimination of undifferentiated cells. A major limitation for cardlomyocyte purification Is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamlne methyl ester Perchlorate, could be used to selectively mark embryonic and neonatal rat cardlomyocytes, as well as mouse, marmoset and human PSC-derlved cardlomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardlomyocytes transplanted Into testes did not Induce teratoma formation. Moreover, aggregate formation of PSC-derlved cardlomyocytes through homophlllc cell-cell adhesion Improved their survival In the lmmunodefident mouse heart. Our approaches will aid In the future success of using PSC-derlved cardlomyocytes for basic and clinical applications.

Original language	English
Pages (from-to)	61-66
Number of pages	6
Journal	Nature Methods
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/nmeth.1403
Publication status	Published - 01-2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1403

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Hattori, F., Chen, H., Yamashita, H., Tohyama, S., Satoh, Y. S., Yuasa, S., Li, W., Yamakawa, H., Tanaka, T., Onitsuka, T., Shimoji, K., Ohno, Y., Egashira, T., Kaneda, R., Murata, M., Hidaka, K., Morisaki, T., Sasaki, E., Suzuki, T., ... Fukuda, K. (2010). Nongenetic method for purifying stem cell-derived cardiomyocytes. Nature Methods, 7(1), 61-66. https://doi.org/10.1038/nmeth.1403

@article{11abefc4ae18428fb65410502548c754,

title = "Nongenetic method for purifying stem cell-derived cardiomyocytes",

abstract = "Several applications of pluripotent stem cell (PSC)-derlved cardlomyocytes require elimination of undifferentiated cells. A major limitation for cardlomyocyte purification Is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamlne methyl ester Perchlorate, could be used to selectively mark embryonic and neonatal rat cardlomyocytes, as well as mouse, marmoset and human PSC-derlved cardlomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardlomyocytes transplanted Into testes did not Induce teratoma formation. Moreover, aggregate formation of PSC-derlved cardlomyocytes through homophlllc cell-cell adhesion Improved their survival In the lmmunodefident mouse heart. Our approaches will aid In the future success of using PSC-derlved cardlomyocytes for basic and clinical applications.",

author = "Fumiyuki Hattori and Hao Chen and Hiromi Yamashita and Shugo Tohyama and Satoh, {Yu Suke} and Shinsuke Yuasa and Weizhen Li and Hiroyuki Yamakawa and Tomofumi Tanaka and Takeshi Onitsuka and Kenichiro Shimoji and Yohei Ohno and Toru Egashira and Ruri Kaneda and Mitsushige Murata and Kyoko Hidaka and Takayuki Morisaki and Erika Sasaki and Takeshi Suzuki and Motoaki Sano and Shinji Makino and Shinzo Oikawa and Keiichi Fukuda",

year = "2010",

month = jan,

doi = "10.1038/nmeth.1403",

language = "English",

volume = "7",

pages = "61--66",

journal = "Nature Methods",

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Hattori, F, Chen, H, Yamashita, H, Tohyama, S, Satoh, YS, Yuasa, S, Li, W, Yamakawa, H, Tanaka, T, Onitsuka, T, Shimoji, K, Ohno, Y, Egashira, T, Kaneda, R, Murata, M, Hidaka, K, Morisaki, T, Sasaki, E, Suzuki, T, Sano, M, Makino, S, Oikawa, S & Fukuda, K 2010, 'Nongenetic method for purifying stem cell-derived cardiomyocytes', Nature Methods, vol. 7, no. 1, pp. 61-66. https://doi.org/10.1038/nmeth.1403

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T1 - Nongenetic method for purifying stem cell-derived cardiomyocytes

AU - Hattori, Fumiyuki

AU - Chen, Hao

AU - Yamashita, Hiromi

AU - Tohyama, Shugo

AU - Satoh, Yu Suke

AU - Yuasa, Shinsuke

AU - Li, Weizhen

AU - Yamakawa, Hiroyuki

AU - Tanaka, Tomofumi

AU - Onitsuka, Takeshi

AU - Shimoji, Kenichiro

AU - Ohno, Yohei

AU - Egashira, Toru

AU - Kaneda, Ruri

AU - Murata, Mitsushige

AU - Hidaka, Kyoko

AU - Morisaki, Takayuki

AU - Sasaki, Erika

AU - Suzuki, Takeshi

AU - Sano, Motoaki

AU - Makino, Shinji

AU - Oikawa, Shinzo

AU - Fukuda, Keiichi

PY - 2010/1

Y1 - 2010/1

N2 - Several applications of pluripotent stem cell (PSC)-derlved cardlomyocytes require elimination of undifferentiated cells. A major limitation for cardlomyocyte purification Is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamlne methyl ester Perchlorate, could be used to selectively mark embryonic and neonatal rat cardlomyocytes, as well as mouse, marmoset and human PSC-derlved cardlomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardlomyocytes transplanted Into testes did not Induce teratoma formation. Moreover, aggregate formation of PSC-derlved cardlomyocytes through homophlllc cell-cell adhesion Improved their survival In the lmmunodefident mouse heart. Our approaches will aid In the future success of using PSC-derlved cardlomyocytes for basic and clinical applications.

AB - Several applications of pluripotent stem cell (PSC)-derlved cardlomyocytes require elimination of undifferentiated cells. A major limitation for cardlomyocyte purification Is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamlne methyl ester Perchlorate, could be used to selectively mark embryonic and neonatal rat cardlomyocytes, as well as mouse, marmoset and human PSC-derlved cardlomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardlomyocytes transplanted Into testes did not Induce teratoma formation. Moreover, aggregate formation of PSC-derlved cardlomyocytes through homophlllc cell-cell adhesion Improved their survival In the lmmunodefident mouse heart. Our approaches will aid In the future success of using PSC-derlved cardlomyocytes for basic and clinical applications.

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JF - Nature Methods

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Nongenetic method for purifying stem cell-derived cardiomyocytes

Abstract

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