TY - JOUR
T1 - Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe
AU - Wada, Y.
AU - Yagihashi, A.
AU - Kameshima, H.
AU - Matsuura, A.
AU - Sato, N.
AU - Kikuchi, K.
AU - Yajima, T.
AU - Sasaki, K.
AU - Uehara, N.
AU - Watanabe, N.
AU - Hirata, K.
PY - 1997
Y1 - 1997
N2 - We have attempted to detect telomerase activity by Southern blot analysis using a digoxigenin labeled probe (5'-(CCCTTA)6CCCTAA-3'). Telomerase activity was detected in all cancer cell lines that were tested, and:the lower limit for detection was 103 cells. Although telomerase activity was detected in colon cancer tissues, it was not found in adenoma or normal tissues. Telomerase activity was completely abrogated by heat and RNase treatment of the CHAPS extract. Our results demonstrate that this nonradioisotopic assay is safe and reliable for detecting telomerase activity in cancer cell lines and biopsy samples.
AB - We have attempted to detect telomerase activity by Southern blot analysis using a digoxigenin labeled probe (5'-(CCCTTA)6CCCTAA-3'). Telomerase activity was detected in all cancer cell lines that were tested, and:the lower limit for detection was 103 cells. Although telomerase activity was detected in colon cancer tissues, it was not found in adenoma or normal tissues. Telomerase activity was completely abrogated by heat and RNase treatment of the CHAPS extract. Our results demonstrate that this nonradioisotopic assay is safe and reliable for detecting telomerase activity in cancer cell lines and biopsy samples.
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U2 - 10.3109/08923979709007667
DO - 10.3109/08923979709007667
M3 - Article
C2 - 9436045
AN - SCOPUS:0031431465
SN - 0892-3973
VL - 19
SP - 451
EP - 457
JO - Immunopharmacology and Immunotoxicology
JF - Immunopharmacology and Immunotoxicology
IS - 4
ER -
