Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe

Y. Wada, A. Yagihashi, H. Kameshima, A. Matsuura, N. Sato, K. Kikuchi, T. Yajima, K. Sasaki, N. Uehara, N. Watanabe, K. Hirata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have attempted to detect telomerase activity by Southern blot analysis using a digoxigenin labeled probe (5'-(CCCTTA)6CCCTAA-3'). Telomerase activity was detected in all cancer cell lines that were tested, and:the lower limit for detection was 103 cells. Although telomerase activity was detected in colon cancer tissues, it was not found in adenoma or normal tissues. Telomerase activity was completely abrogated by heat and RNase treatment of the CHAPS extract. Our results demonstrate that this nonradioisotopic assay is safe and reliable for detecting telomerase activity in cancer cell lines and biopsy samples.

Original languageEnglish
Pages (from-to)451-457
Number of pages7
JournalImmunopharmacology and Immunotoxicology
Volume19
Issue number4
DOIs
Publication statusPublished - 01-01-1997

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Digoxigenin
Telomerase
Amplification
Cells
Tissue
Cell Line
Biopsy
Ribonucleases
Southern Blotting
Adenoma
Colonic Neoplasms
Limit of Detection
Assays
Neoplasms
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Toxicology
  • Pharmacology

Cite this

Wada, Y. ; Yagihashi, A. ; Kameshima, H. ; Matsuura, A. ; Sato, N. ; Kikuchi, K. ; Yajima, T. ; Sasaki, K. ; Uehara, N. ; Watanabe, N. ; Hirata, K. / Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe. In: Immunopharmacology and Immunotoxicology. 1997 ; Vol. 19, No. 4. pp. 451-457.
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Wada, Y, Yagihashi, A, Kameshima, H, Matsuura, A, Sato, N, Kikuchi, K, Yajima, T, Sasaki, K, Uehara, N, Watanabe, N & Hirata, K 1997, 'Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe', Immunopharmacology and Immunotoxicology, vol. 19, no. 4, pp. 451-457. https://doi.org/10.3109/08923979709007667

Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe. / Wada, Y.; Yagihashi, A.; Kameshima, H.; Matsuura, A.; Sato, N.; Kikuchi, K.; Yajima, T.; Sasaki, K.; Uehara, N.; Watanabe, N.; Hirata, K.

In: Immunopharmacology and Immunotoxicology, Vol. 19, No. 4, 01.01.1997, p. 451-457.

Research output: Contribution to journalArticle

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T1 - Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe

AU - Wada, Y.

AU - Yagihashi, A.

AU - Kameshima, H.

AU - Matsuura, A.

AU - Sato, N.

AU - Kikuchi, K.

AU - Yajima, T.

AU - Sasaki, K.

AU - Uehara, N.

AU - Watanabe, N.

AU - Hirata, K.

PY - 1997/1/1

Y1 - 1997/1/1

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