Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe

Y. Wada; A. Yagihashi; H. Kameshima; A. Matsuura; N. Sato; K. Kikuchi; T. Yajima; K. Sasaki; N. Uehara; N. Watanabe; K. Hirata

doi:10.3109/08923979709007667

Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe

Y. Wada
, A. Yagihashi
, H. Kameshima
, A. Matsuura
, N. Sato
, K. Kikuchi
, T. Yajima
, K. Sasaki
, N. Uehara
, N. Watanabe
, K. Hirata

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We have attempted to detect telomerase activity by Southern blot analysis using a digoxigenin labeled probe (5'-(CCCTTA)₆CCCTAA-3'). Telomerase activity was detected in all cancer cell lines that were tested, and:the lower limit for detection was 10³ cells. Although telomerase activity was detected in colon cancer tissues, it was not found in adenoma or normal tissues. Telomerase activity was completely abrogated by heat and RNase treatment of the CHAPS extract. Our results demonstrate that this nonradioisotopic assay is safe and reliable for detecting telomerase activity in cancer cell lines and biopsy samples.

Original language	English
Pages (from-to)	451-457
Number of pages	7
Journal	Immunopharmacology and Immunotoxicology
Volume	19
Issue number	4
DOIs	https://doi.org/10.3109/08923979709007667
Publication status	Published - 1997
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Toxicology
Pharmacology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/08923979709007667

Cite this

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title = "Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe",

abstract = "We have attempted to detect telomerase activity by Southern blot analysis using a digoxigenin labeled probe (5'-(CCCTTA)6CCCTAA-3'). Telomerase activity was detected in all cancer cell lines that were tested, and:the lower limit for detection was 103 cells. Although telomerase activity was detected in colon cancer tissues, it was not found in adenoma or normal tissues. Telomerase activity was completely abrogated by heat and RNase treatment of the CHAPS extract. Our results demonstrate that this nonradioisotopic assay is safe and reliable for detecting telomerase activity in cancer cell lines and biopsy samples.",

author = "Y. Wada and A. Yagihashi and H. Kameshima and A. Matsuura and N. Sato and K. Kikuchi and T. Yajima and K. Sasaki and N. Uehara and N. Watanabe and K. Hirata",

year = "1997",

doi = "10.3109/08923979709007667",

language = "English",

volume = "19",

pages = "451--457",

journal = "Immunopharmacology and Immunotoxicology",

issn = "0892-3973",

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number = "4",

}

TY - JOUR

T1 - Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe

AU - Wada, Y.

AU - Yagihashi, A.

AU - Kameshima, H.

AU - Matsuura, A.

AU - Sato, N.

AU - Kikuchi, K.

AU - Yajima, T.

AU - Sasaki, K.

AU - Uehara, N.

AU - Watanabe, N.

AU - Hirata, K.

PY - 1997

Y1 - 1997

N2 - We have attempted to detect telomerase activity by Southern blot analysis using a digoxigenin labeled probe (5'-(CCCTTA)6CCCTAA-3'). Telomerase activity was detected in all cancer cell lines that were tested, and:the lower limit for detection was 103 cells. Although telomerase activity was detected in colon cancer tissues, it was not found in adenoma or normal tissues. Telomerase activity was completely abrogated by heat and RNase treatment of the CHAPS extract. Our results demonstrate that this nonradioisotopic assay is safe and reliable for detecting telomerase activity in cancer cell lines and biopsy samples.

AB - We have attempted to detect telomerase activity by Southern blot analysis using a digoxigenin labeled probe (5'-(CCCTTA)6CCCTAA-3'). Telomerase activity was detected in all cancer cell lines that were tested, and:the lower limit for detection was 103 cells. Although telomerase activity was detected in colon cancer tissues, it was not found in adenoma or normal tissues. Telomerase activity was completely abrogated by heat and RNase treatment of the CHAPS extract. Our results demonstrate that this nonradioisotopic assay is safe and reliable for detecting telomerase activity in cancer cell lines and biopsy samples.

UR - https://www.scopus.com/pages/publications/0031431465

UR - https://www.scopus.com/pages/publications/0031431465#tab=citedBy

U2 - 10.3109/08923979709007667

DO - 10.3109/08923979709007667

M3 - Article

C2 - 9436045

AN - SCOPUS:0031431465

SN - 0892-3973

VL - 19

SP - 451

EP - 457

JO - Immunopharmacology and Immunotoxicology

JF - Immunopharmacology and Immunotoxicology

IS - 4

ER -

Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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