TY - JOUR
T1 - Novel Blood Purification System for Regulating Excessive Immune Reactions in Severe Sepsis and Septic Shock
T2 - An Ex Vivo Pilot Study
AU - Hara, Yoshitaka
AU - Shimomura, Yasuyo
AU - Nakamura, Tomoyuki
AU - Kuriyama, Naohide
AU - Yamashita, Chizuru
AU - Kato, Yu
AU - Miyasho, Taku
AU - Sakai, Toshikazu
AU - Yamada, Shingo
AU - Moriyama, Kazuhiro
AU - Nishida, Osamu
N1 - Publisher Copyright:
© 2015 International Society for Apheresis, Japanese Society for Apheresis, and Japanese Society of Dialysis Therapy.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Promising results have been reported with blood purification as adjuvant treatment; however, the immunological mechanisms remain unclear. We have been developing a new blood purification system for regulating excessive immune reactions in severe sepsis and septic shock using a granulocyte adsorbing column (Adacolumn [Ada]), and a cytokine-adsorbing hemofilter (AN69ST hemofilter [AN69]). Fresh porcine blood was circulated for 6h in five experimental groups including Ada and AN69 to assess the effects of leukocyte adsorption, phagocytic activity and adhesiveness of granulocytes. In the present study, we found that Ada mainly adsorbed granulocytes and monocytes, but not lymphocytes. The phagocytic activity level of granulocytes decreased, and adhesiveness increased, but the number of CD11b-positive cells markedly decreased in the current system. Elevated cytokine levels (IL-1β, IL-8 and IL-10) at the outlet of Ada were significantly lower than at the outlet of AN69 due to cytokine adsorption. Further studies are needed to better understand cellular interactions.
AB - Promising results have been reported with blood purification as adjuvant treatment; however, the immunological mechanisms remain unclear. We have been developing a new blood purification system for regulating excessive immune reactions in severe sepsis and septic shock using a granulocyte adsorbing column (Adacolumn [Ada]), and a cytokine-adsorbing hemofilter (AN69ST hemofilter [AN69]). Fresh porcine blood was circulated for 6h in five experimental groups including Ada and AN69 to assess the effects of leukocyte adsorption, phagocytic activity and adhesiveness of granulocytes. In the present study, we found that Ada mainly adsorbed granulocytes and monocytes, but not lymphocytes. The phagocytic activity level of granulocytes decreased, and adhesiveness increased, but the number of CD11b-positive cells markedly decreased in the current system. Elevated cytokine levels (IL-1β, IL-8 and IL-10) at the outlet of Ada were significantly lower than at the outlet of AN69 due to cytokine adsorption. Further studies are needed to better understand cellular interactions.
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U2 - 10.1111/1744-9987.12338
DO - 10.1111/1744-9987.12338
M3 - Article
C2 - 26386217
AN - SCOPUS:84941907184
SN - 1744-9979
VL - 19
SP - 308
EP - 315
JO - Therapeutic Apheresis and Dialysis
JF - Therapeutic Apheresis and Dialysis
IS - 4
ER -