Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue

Shigejiro Iwashima, Takenori Ozaki, Shoichi Maruyama, Yousuke Saka, Masato Kobori, Kaoru Omae, Hirotake Yamaguchi, Tomoaki Niimi, Kazuhiro Toriyama, Yuzuru Kamei, Shuhei Torii, Toyoaki Murohara, Yukio Yuzawa, Yasuo Kitagawa, Seiichi Matsuo

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Accumulating evidence suggests that the delivery of human adipose tissue-derived stromal cells (hASCs) has great potential as regenerative therapy. This was performed to develop a method for expanding hASCs by reducing the amount of serum required. We demonstrate that hASCs were able to expand efficiently in media containing 2% serum and fibroblast growth factor-2. These cells, or low serum cultured hASCs (hLASCs), expressed cell surface markers similar to those on bone marrow-derived mesenchymal stem cells, and could be differentiated into cells of mesenchymal lineage. Of interest, hLASCs secreted higher levels of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) than hASCs cultured in 20% serum (hHASCs). Moreover, hLASC-conditioned media significantly increased endothelial cell (EC) proliferation and decreased EC apoptosis compared to that obtained from hHASCs or control media only. Antibodies against VEGF and HGF virtually negated these effects. When hASCs were administered into the ischemic hindlimbs of nude rats, hLASCs improved blood flow, increased capillary density, and raised the levels of VEGF and HGF in the muscles as compared with hHASCs. In conclusion, we demonstrate a novel low serum culture system for hASCs, which may have great potential in regenerative cell therapy for damaged organs in the clinical setting.

Original languageEnglish
Pages (from-to)533-543
Number of pages11
JournalStem Cells and Development
Volume18
Issue number4
DOIs
Publication statusPublished - 01-05-2009

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Subcutaneous Fat
Mesenchymal Stromal Cells
Stromal Cells
Adipose Tissue
Serum
Vascular Endothelial Growth Factor A
Hepatocyte Growth Factor
Endothelial Cells
Nude Rats
Fibroblast Growth Factor 2
Cell Lineage
Hindlimb
Conditioned Culture Medium
Cell- and Tissue-Based Therapy
Bone Marrow
Cell Proliferation
Apoptosis
Muscles
Antibodies

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Iwashima, S., Ozaki, T., Maruyama, S., Saka, Y., Kobori, M., Omae, K., ... Matsuo, S. (2009). Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue. Stem Cells and Development, 18(4), 533-543. https://doi.org/10.1089/scd.2008.0358
Iwashima, Shigejiro ; Ozaki, Takenori ; Maruyama, Shoichi ; Saka, Yousuke ; Kobori, Masato ; Omae, Kaoru ; Yamaguchi, Hirotake ; Niimi, Tomoaki ; Toriyama, Kazuhiro ; Kamei, Yuzuru ; Torii, Shuhei ; Murohara, Toyoaki ; Yuzawa, Yukio ; Kitagawa, Yasuo ; Matsuo, Seiichi. / Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue. In: Stem Cells and Development. 2009 ; Vol. 18, No. 4. pp. 533-543.
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Iwashima, S, Ozaki, T, Maruyama, S, Saka, Y, Kobori, M, Omae, K, Yamaguchi, H, Niimi, T, Toriyama, K, Kamei, Y, Torii, S, Murohara, T, Yuzawa, Y, Kitagawa, Y & Matsuo, S 2009, 'Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue', Stem Cells and Development, vol. 18, no. 4, pp. 533-543. https://doi.org/10.1089/scd.2008.0358

Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue. / Iwashima, Shigejiro; Ozaki, Takenori; Maruyama, Shoichi; Saka, Yousuke; Kobori, Masato; Omae, Kaoru; Yamaguchi, Hirotake; Niimi, Tomoaki; Toriyama, Kazuhiro; Kamei, Yuzuru; Torii, Shuhei; Murohara, Toyoaki; Yuzawa, Yukio; Kitagawa, Yasuo; Matsuo, Seiichi.

In: Stem Cells and Development, Vol. 18, No. 4, 01.05.2009, p. 533-543.

Research output: Contribution to journalArticle

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T1 - Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue

AU - Iwashima, Shigejiro

AU - Ozaki, Takenori

AU - Maruyama, Shoichi

AU - Saka, Yousuke

AU - Kobori, Masato

AU - Omae, Kaoru

AU - Yamaguchi, Hirotake

AU - Niimi, Tomoaki

AU - Toriyama, Kazuhiro

AU - Kamei, Yuzuru

AU - Torii, Shuhei

AU - Murohara, Toyoaki

AU - Yuzawa, Yukio

AU - Kitagawa, Yasuo

AU - Matsuo, Seiichi

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Accumulating evidence suggests that the delivery of human adipose tissue-derived stromal cells (hASCs) has great potential as regenerative therapy. This was performed to develop a method for expanding hASCs by reducing the amount of serum required. We demonstrate that hASCs were able to expand efficiently in media containing 2% serum and fibroblast growth factor-2. These cells, or low serum cultured hASCs (hLASCs), expressed cell surface markers similar to those on bone marrow-derived mesenchymal stem cells, and could be differentiated into cells of mesenchymal lineage. Of interest, hLASCs secreted higher levels of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) than hASCs cultured in 20% serum (hHASCs). Moreover, hLASC-conditioned media significantly increased endothelial cell (EC) proliferation and decreased EC apoptosis compared to that obtained from hHASCs or control media only. Antibodies against VEGF and HGF virtually negated these effects. When hASCs were administered into the ischemic hindlimbs of nude rats, hLASCs improved blood flow, increased capillary density, and raised the levels of VEGF and HGF in the muscles as compared with hHASCs. In conclusion, we demonstrate a novel low serum culture system for hASCs, which may have great potential in regenerative cell therapy for damaged organs in the clinical setting.

AB - Accumulating evidence suggests that the delivery of human adipose tissue-derived stromal cells (hASCs) has great potential as regenerative therapy. This was performed to develop a method for expanding hASCs by reducing the amount of serum required. We demonstrate that hASCs were able to expand efficiently in media containing 2% serum and fibroblast growth factor-2. These cells, or low serum cultured hASCs (hLASCs), expressed cell surface markers similar to those on bone marrow-derived mesenchymal stem cells, and could be differentiated into cells of mesenchymal lineage. Of interest, hLASCs secreted higher levels of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) than hASCs cultured in 20% serum (hHASCs). Moreover, hLASC-conditioned media significantly increased endothelial cell (EC) proliferation and decreased EC apoptosis compared to that obtained from hHASCs or control media only. Antibodies against VEGF and HGF virtually negated these effects. When hASCs were administered into the ischemic hindlimbs of nude rats, hLASCs improved blood flow, increased capillary density, and raised the levels of VEGF and HGF in the muscles as compared with hHASCs. In conclusion, we demonstrate a novel low serum culture system for hASCs, which may have great potential in regenerative cell therapy for damaged organs in the clinical setting.

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