Novel NBS1 heterozygous germ line mutation causing MRE11-binding domain loss predisposes to common types of cancer

Hiromichi Ebi, Keitaro Matsuo, Nobuyoshi Sugito, Motoshi Suzuki, Hirotaka Osada, Kazuo Tajima, Ryuzo Ueda, Takashi Takahashi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

DNA damage response (DDR) pathways maintain genomic stability. A 657del5 mutation of NBS1, a key DDR component, causing the rare cancer-predisposing Nijmegen breakage syndrome has been reported nearly exclusively in Slavic populations. In this study, we describe the first identification in a Japanese population of an unprecedented type of heterozygous NBS1 mutant, termed IVS11+2insT, lacking the MRE11- and ATM-binding site at the COOH terminus. Profoundly defective in crucial binding to MRE11, MDC1, BRCA1, and wild-type NBS1, the mutant caused impaired ATM phosphorylation in response to low-dose irradiation in a heterozygous state. Importantly, whereas IVS11+2insT was found in only 2 (0.09%) of 2,348 control subjects, it was identified in 2% (2 of 96) of heterozygotes with gastric cancer, 0.8% (3 of 376) of those with colorectal cancer, and 0.4% (2 of 532) of those with lung cancer, which were comparable to frequencies reported for other DDR-related genes known to confer cancer susceptibility. The presence of the heterozygous IVS11+2insT mutation seemed to be associated with an increased riskfor gastrointestinal cancers, with an odds ratio of 12.6 and 95% confidence interval (95% CI) of 2.05 to 132.1 (P = 0.0001). The odds ratios separately calculated for gastric and colorectal cancers were 25.0 (95% CI, 1.78-346.0) and 9.43 (95% CI, 1.08-113.1), respectively. These findings suggest that IVS11+2insT is associated with an increased riskfor the development of certain types of common cancers, warranting future investigation including detailed phenotypic characterization of age of onset and penetrance in heterozygotes, as well as screening in other ethnic groups.

Original languageEnglish
Pages (from-to)11158-11165
Number of pages8
JournalCancer Research
Volume67
Issue number23
DOIs
Publication statusPublished - 01-12-2007

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Germ-Line Mutation
DNA Damage
Confidence Intervals
Heterozygote
Stomach Neoplasms
Colorectal Neoplasms
Nijmegen Breakage Syndrome
Odds Ratio
Neoplasms
Mutation
Gastrointestinal Neoplasms
Penetrance
Genomic Instability
Age of Onset
Ethnic Groups
Population
Lung Neoplasms
Binding Sites
Phosphorylation
Genes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Ebi, Hiromichi ; Matsuo, Keitaro ; Sugito, Nobuyoshi ; Suzuki, Motoshi ; Osada, Hirotaka ; Tajima, Kazuo ; Ueda, Ryuzo ; Takahashi, Takashi. / Novel NBS1 heterozygous germ line mutation causing MRE11-binding domain loss predisposes to common types of cancer. In: Cancer Research. 2007 ; Vol. 67, No. 23. pp. 11158-11165.
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abstract = "DNA damage response (DDR) pathways maintain genomic stability. A 657del5 mutation of NBS1, a key DDR component, causing the rare cancer-predisposing Nijmegen breakage syndrome has been reported nearly exclusively in Slavic populations. In this study, we describe the first identification in a Japanese population of an unprecedented type of heterozygous NBS1 mutant, termed IVS11+2insT, lacking the MRE11- and ATM-binding site at the COOH terminus. Profoundly defective in crucial binding to MRE11, MDC1, BRCA1, and wild-type NBS1, the mutant caused impaired ATM phosphorylation in response to low-dose irradiation in a heterozygous state. Importantly, whereas IVS11+2insT was found in only 2 (0.09{\%}) of 2,348 control subjects, it was identified in 2{\%} (2 of 96) of heterozygotes with gastric cancer, 0.8{\%} (3 of 376) of those with colorectal cancer, and 0.4{\%} (2 of 532) of those with lung cancer, which were comparable to frequencies reported for other DDR-related genes known to confer cancer susceptibility. The presence of the heterozygous IVS11+2insT mutation seemed to be associated with an increased riskfor gastrointestinal cancers, with an odds ratio of 12.6 and 95{\%} confidence interval (95{\%} CI) of 2.05 to 132.1 (P = 0.0001). The odds ratios separately calculated for gastric and colorectal cancers were 25.0 (95{\%} CI, 1.78-346.0) and 9.43 (95{\%} CI, 1.08-113.1), respectively. These findings suggest that IVS11+2insT is associated with an increased riskfor the development of certain types of common cancers, warranting future investigation including detailed phenotypic characterization of age of onset and penetrance in heterozygotes, as well as screening in other ethnic groups.",
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Novel NBS1 heterozygous germ line mutation causing MRE11-binding domain loss predisposes to common types of cancer. / Ebi, Hiromichi; Matsuo, Keitaro; Sugito, Nobuyoshi; Suzuki, Motoshi; Osada, Hirotaka; Tajima, Kazuo; Ueda, Ryuzo; Takahashi, Takashi.

In: Cancer Research, Vol. 67, No. 23, 01.12.2007, p. 11158-11165.

Research output: Contribution to journalArticle

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AU - Ebi, Hiromichi

AU - Matsuo, Keitaro

AU - Sugito, Nobuyoshi

AU - Suzuki, Motoshi

AU - Osada, Hirotaka

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AB - DNA damage response (DDR) pathways maintain genomic stability. A 657del5 mutation of NBS1, a key DDR component, causing the rare cancer-predisposing Nijmegen breakage syndrome has been reported nearly exclusively in Slavic populations. In this study, we describe the first identification in a Japanese population of an unprecedented type of heterozygous NBS1 mutant, termed IVS11+2insT, lacking the MRE11- and ATM-binding site at the COOH terminus. Profoundly defective in crucial binding to MRE11, MDC1, BRCA1, and wild-type NBS1, the mutant caused impaired ATM phosphorylation in response to low-dose irradiation in a heterozygous state. Importantly, whereas IVS11+2insT was found in only 2 (0.09%) of 2,348 control subjects, it was identified in 2% (2 of 96) of heterozygotes with gastric cancer, 0.8% (3 of 376) of those with colorectal cancer, and 0.4% (2 of 532) of those with lung cancer, which were comparable to frequencies reported for other DDR-related genes known to confer cancer susceptibility. The presence of the heterozygous IVS11+2insT mutation seemed to be associated with an increased riskfor gastrointestinal cancers, with an odds ratio of 12.6 and 95% confidence interval (95% CI) of 2.05 to 132.1 (P = 0.0001). The odds ratios separately calculated for gastric and colorectal cancers were 25.0 (95% CI, 1.78-346.0) and 9.43 (95% CI, 1.08-113.1), respectively. These findings suggest that IVS11+2insT is associated with an increased riskfor the development of certain types of common cancers, warranting future investigation including detailed phenotypic characterization of age of onset and penetrance in heterozygotes, as well as screening in other ethnic groups.

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