Novel pulse duplicating bioreactor system for tissue-engineered vascular construct

Yuji Narita, Ken Ichiro Hata, Hideaki Kagami, Akihiko Usui, Minoru Ueda, Yuichi Ueda

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)


Cell culture in a biomimetic environment is known to improve the mechanical endurance of tissue-engineered cardiovascular components. Our goal was to generate a bioreactor that can reproduce a wide range of pulsatile flows with a completely physiological pressure profile. The morphology and biochemical properties of tissue-engineered products were also studied to test the usefulness of this novel bioreactor. The combination of an outflow valve, compliance chamber, and resistant clamps together with a balloon pumping system was able to successfully reproduce both physiological systolic and diastolic pressures. The compliance chamber was especially effective in transforming the original peaky pressure waveform into a physiological pressure profile. The tissues, cultured under a physiological pressure waveform with pulsatile flow, presented widely distributed cells in close contact with each other. They also showed significantly higher cell numbers, total protein content, and proteoglycan-glycosaminoglycan content than cultured tissues under a peaky pressure wave or under static conditions. This new bioreactor system is suitable for evaluating a favorable environment for tissue-engineered cardiovascular components.

Original languageEnglish
Pages (from-to)1224-1233
Number of pages10
JournalTissue Engineering
Issue number7-8
Publication statusPublished - 07-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Cell Biology


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