Novel therapy for hearing loss: Delivery of insulin-like growth factor 1 to the cochlea using gelatin hydrogel

Kyu Yup Lee, Takayuki Nakagawa, Takayuki Okano, Ryusuke Hori, Kazuya Ono, Yasuhiko Tabata, Sang Heun Lee, Juichi Ito

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)

Abstract

HYPOTHESIS: Local application of recombinant human insulin-like growth factor 1 (rhIGF-1) via a biodegradable hydrogel after onset of noise-induced hearing loss (NIHL) can attenuate functional and histologic damage. BACKGROUND: The biodegradable gelatin hydrogel makes a complex with drugs by static electric charges and releases drugs by degradation of gelatin polymers. We previously demonstrated the efficacy of local rhIGF-1 application via hydrogels before noise exposure for prevention of NIHL. METHODS: First, we used an enzyme-linked immunosorbent assay to measure human IGF-1 concentrations in the cochlear fluid after placing a hydrogel containing rhIGF-1 onto the round window membrane of guinea pigs. Second, the functionality and the histology of guinea pig cochleae treated with local rhIGF-1 application at different concentrations after noise exposure were examined. Control animals were treated with a hydrogel immersed in physiologic saline alone. RESULTS: The results revealed sustained delivery of rhIGF-1 into the cochlear fluid via the hydrogel. The measurement of auditory brainstem responses demonstrated that local rhIGF-1 treatment significantly reduced the threshold elevation from noise. Histologic analysis exhibited increased survival of outer hair cells by local rhIGF-1 application through the hydrogel. CONCLUSION: These findings indicate that local rhIGF-1 treatment via gelatin hydrogels is effective for treatment of NIHL.

Original languageEnglish
Pages (from-to)976-981
Number of pages6
JournalOtology and Neurotology
Volume28
Issue number7
DOIs
Publication statusPublished - 10-2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Sensory Systems
  • Clinical Neurology

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